Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103020 |
| Name | oriT_pA31-12  |
| Organism | Escherichia coli strain A31-12 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX034083 (18155..18207 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pA31-12
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1952 | GenBank | WP_058153281 |
| Name | t4cp2_HTH94_RS00295_pA31-12 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73333.86 Da Isoelectric Point: 9.1391
>WP_058153281.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 38969..62514
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTH94_RS00225 (pA31-12_050) | 34294..34947 | - | 654 | WP_015387348 | hypothetical protein | - |
| HTH94_RS00230 (pA31-12_051) | 34959..36083 | - | 1125 | WP_000486719 | site-specific integrase | - |
| HTH94_RS00235 | 36418..36747 | - | 330 | WP_096217466 | pilus assembly protein | - |
| HTH94_RS00465 | 36723..36944 | + | 222 | Protein_48 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH94_RS00245 (pA31-12_053) | 36941..38317 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH94_RS00250 (pA31-12_054) | 38330..38965 | - | 636 | WP_000934978 | A24 family peptidase | - |
| HTH94_RS00255 (pA31-12_055) | 38969..39397 | - | 429 | WP_001326801 | lytic transglycosylase domain-containing protein | virB1 |
| HTH94_RS00260 (pA31-12_056) | 39518..40075 | - | 558 | WP_000095051 | type 4 pilus major pilin | - |
| HTH94_RS00265 (pA31-12_057) | 40120..41229 | - | 1110 | WP_000974900 | type II secretion system F family protein | - |
| HTH94_RS00270 (pA31-12_058) | 41220..42758 | - | 1539 | WP_001420475 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTH94_RS00275 (pA31-12_059) | 42783..43277 | - | 495 | WP_000912551 | type IV pilus biogenesis protein PilP | - |
| HTH94_RS00280 (pA31-12_060) | 43261..44583 | - | 1323 | WP_000454143 | type 4b pilus protein PilO2 | - |
| HTH94_RS00285 (pA31-12_061) | 44622..46265 | - | 1644 | WP_001035590 | PilN family type IVB pilus formation outer membrane protein | - |
| HTH94_RS00290 (pA31-12_062) | 46258..46776 | - | 519 | WP_015387353 | YfdA protein | virb4 |
| HTH94_RS00295 (pA31-12_063) | 46823..48781 | - | 1959 | WP_058153281 | type IV secretory system conjugative DNA transfer family protein | - |
| HTH94_RS00300 (pA31-12_064) | 48797..49852 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTH94_RS00305 (pA31-12_065) | 49871..51010 | - | 1140 | WP_015387354 | TrbI/VirB10 family protein | virB10 |
| HTH94_RS00310 (pA31-12_066) | 51000..51701 | - | 702 | WP_053882504 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTH94_RS00315 (pA31-12_067) | 51767..52501 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTH94_RS00320 (pA31-12_068) | 52665..55022 | - | 2358 | WP_015387356 | VirB4 family type IV secretion system protein | virb4 |
| HTH94_RS00325 (pA31-12_069) | 55028..55348 | - | 321 | WP_000362081 | VirB3 family type IV secretion system protein | virB3 |
| HTH94_RS00460 (pA31-12_070) | 55419..55709 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTH94_RS00335 (pA31-12_071) | 55709..56293 | - | 585 | WP_001401693 | lytic transglycosylase domain-containing protein | virB1 |
| HTH94_RS00340 (pA31-12_072) | 56314..56712 | - | 399 | WP_001708012 | hypothetical protein | - |
| HTH94_RS00345 (pA31-12_074) | 57052..57489 | - | 438 | WP_015387358 | type IV pilus biogenesis protein PilM | - |
| HTH94_RS00350 (pA31-12_075) | 57495..58730 | - | 1236 | WP_001419735 | TcpQ domain-containing protein | - |
| HTH94_RS00355 (pA31-12_076) | 58733..59032 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTH94_RS00360 (pA31-12_077) | 59099..59731 | - | 633 | WP_001419736 | hypothetical protein | - |
| HTH94_RS00365 (pA31-12_078) | 59779..60588 | + | 810 | WP_001419737 | DUF5710 domain-containing protein | - |
| HTH94_RS00370 (pA31-12_079) | 60635..60871 | - | 237 | WP_000750964 | EexN family lipoprotein | - |
| HTH94_RS00375 (pA31-12_080) | 60875..61522 | - | 648 | WP_001419738 | type IV secretion system protein | - |
| HTH94_RS00380 (pA31-12_0081) | 61528..62514 | - | 987 | WP_001419739 | type IV secretion system protein | virB6 |
| HTH94_RS00385 (pA31-12_0082) | 62518..62775 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTH94_RS00390 (pA31-12_083) | 62772..63074 | - | 303 | WP_001360345 | hypothetical protein | - |
| HTH94_RS00395 (pA31-12_084) | 63090..63341 | - | 252 | WP_015387362 | hypothetical protein | - |
| HTH94_RS00400 (pA31-12_085) | 63338..63520 | - | 183 | WP_022540746 | hypothetical protein | - |
| HTH94_RS00405 (pA31-12_086) | 63489..64151 | - | 663 | WP_001419740 | hypothetical protein | - |
| HTH94_RS00410 (pA31-12_087) | 64162..64332 | - | 171 | WP_000550721 | hypothetical protein | - |
| HTH94_RS00415 (pA31-12_088) | 64336..64779 | - | 444 | WP_000964840 | NfeD family protein | - |
| HTH94_RS00420 (pA31-12_089) | 64852..65805 | - | 954 | WP_072105959 | SPFH domain-containing protein | - |
| HTH94_RS00425 (pA31-12_090) | 65851..66045 | - | 195 | WP_000049865 | DUF1187 family protein | - |
| HTH94_RS00430 (pA31_12_091) | 66038..66544 | - | 507 | WP_001358485 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3463 | GenBank | NZ_KX034083 |
| Plasmid name | pA31-12 | Incompatibility group | IncI2 |
| Plasmid size | 67134 bp | Coordinate of oriT [Strand] | 18155..18207 [-] |
| Host baterium | Escherichia coli strain A31-12 |
Cargo genes
| Drug resistance gene | blaCTX-M-55, mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |