Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103017 |
| Name | oriT_pBA77-MCR-1  |
| Organism | Escherichia coli strain BA77 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KX013539 (15907..15959 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pBA77-MCR-1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1950 | GenBank | WP_015059539 |
| Name | t4cp2_HTH74_RS00245_pBA77-MCR-1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35087..57929
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTH74_RS00180 | 30309..31433 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTH74_RS00405 | 31766..31984 | + | 219 | Protein_39 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH74_RS00190 | 32420..32575 | + | 156 | WP_001358489 | hypothetical protein | - |
| HTH74_RS00195 | 33149..34435 | - | 1287 | WP_104730792 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH74_RS00200 | 34448..35083 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTH74_RS00205 | 35087..35569 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTH74_RS00210 | 35635..36198 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| HTH74_RS00215 | 36248..37357 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTH74_RS00220 | 37348..38886 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTH74_RS00225 | 38911..39405 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTH74_RS00230 | 39389..40711 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTH74_RS00235 | 40750..42393 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTH74_RS00240 | 42386..42934 | - | 549 | WP_172687990 | sigma 54-interacting transcriptional regulator | virb4 |
| HTH74_RS00245 | 42981..44939 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTH74_RS00250 | 44955..46010 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTH74_RS00255 | 46029..47168 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| HTH74_RS00260 | 47158..47859 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTH74_RS00265 | 47925..48659 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTH74_RS00270 | 48825..51182 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| HTH74_RS00275 | 51188..51508 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTH74_RS00400 | 51579..51869 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTH74_RS00285 | 51869..52453 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| HTH74_RS00290 | 52474..52872 | - | 399 | WP_001153669 | hypothetical protein | - |
| HTH74_RS00295 | 52991..53428 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| HTH74_RS00300 | 53434..54669 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| HTH74_RS00305 | 54672..54971 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTH74_RS00310 | 55019..55828 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| HTH74_RS00315 | 56051..56275 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| HTH74_RS00320 | 56284..56928 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| HTH74_RS00325 | 56934..57929 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| HTH74_RS00330 | 57933..58190 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTH74_RS00335 | 58187..58540 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTH74_RS00340 | 58760..59206 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTH74_RS00345 | 59217..59387 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTH74_RS00350 | 59391..59834 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTH74_RS00355 | 60208..61161 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTH74_RS00360 | 61188..61364 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTH74_RS00365 | 61357..61572 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTH74_RS00370 | 61565..62071 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3460 | GenBank | NZ_KX013539 |
| Plasmid name | pBA77-MCR-1 | Incompatibility group | IncI2 |
| Plasmid size | 62661 bp | Coordinate of oriT [Strand] | 15907..15959 [-] |
| Host baterium | Escherichia coli strain BA77 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |