Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103001 |
| Name | oriT_pT15G-1  |
| Organism | Staphylococcus lugdunensis strain Tlug15G-4 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KU882681 (39240..39275 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pT15G-1
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2252 | GenBank | WP_001252101 |
| Name | MobA_MobL_HTH00_RS00215_pT15G-1 |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 1943 | GenBank | WP_000209436 |
| Name | t4cp2_HTH00_RS00085_pT15G-1 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5713..16934
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTH00_RS00010 | 1079..1753 | + | 675 | WP_172687229 | IS6-like element IS257 family transposase | - |
| HTH00_RS00015 (pT15G-1_3) | 2082..2852 | - | 771 | WP_001795128 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | - |
| HTH00_RS00020 (pT15G-1_4) | 3012..3158 | - | 147 | WP_001817822 | hypothetical protein | - |
| HTH00_RS00025 (pT15G-1_5) | 3202..3876 | + | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| HTH00_RS00030 (pT15G-1_6) | 3985..4173 | - | 189 | WP_001063224 | hypothetical protein | - |
| HTH00_RS00035 (pT15G-1_7) | 4345..5319 | + | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| HTH00_RS00040 (pT15G-1_8) | 5336..5653 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| HTH00_RS00045 (pT15G-1_9) | 5713..6120 | + | 408 | WP_000110157 | hypothetical protein | trsC |
| HTH00_RS00050 (pT15G-1_10) | 6107..6790 | + | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| HTH00_RS00055 (pT15G-1_11) | 6805..8823 | + | 2019 | WP_001795400 | DUF87 domain-containing protein | virb4 |
| HTH00_RS00060 (pT15G-1_12) | 8835..10115 | + | 1281 | WP_000702361 | membrane protein | trsF |
| HTH00_RS00065 (pT15G-1_13) | 10133..11209 | + | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| HTH00_RS00070 (pT15G-1_14) | 11219..11704 | + | 486 | WP_000735569 | TrsH/TraH family protein | - |
| HTH00_RS00075 (pT15G-1_15) | 11720..13822 | + | 2103 | WP_001094109 | DNA topoisomerase III | - |
| HTH00_RS00080 (pT15G-1_16) | 13838..14302 | + | 465 | WP_000715273 | hypothetical protein | trsJ |
| HTH00_RS00085 (pT15G-1_17) | 14299..15939 | + | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HTH00_RS00090 (pT15G-1_18) | 16017..16934 | + | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| HTH00_RS00095 (pT15G-1_19) | 16951..17343 | + | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| HTH00_RS00100 (pT15G-1_20) | 17400..17567 | + | 168 | WP_000869301 | hypothetical protein | - |
| HTH00_RS00105 (pT15G-1_21) | 17605..18279 | + | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| HTH00_RS00110 (pT15G-1_22) | 18340..18714 | + | 375 | Protein_21 | IS256 family transposase | - |
| HTH00_RS00115 (pT15G-1_23) | 18844..20283 | - | 1440 | WP_001028144 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| HTH00_RS00120 (pT15G-1_24) | 20284..20676 | - | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| HTH00_RS00125 (pT15G-1_25) | 20733..21107 | - | 375 | Protein_24 | IS256 family transposase | - |
| HTH00_RS00130 (pT15G-1_26) | 21168..21842 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
Host bacterium
| ID | 3444 | GenBank | NZ_KU882681 |
| Plasmid name | pT15G-1 | Incompatibility group | - |
| Plasmid size | 42253 bp | Coordinate of oriT [Strand] | 39240..39275 [+] |
| Host baterium | Staphylococcus lugdunensis strain Tlug15G-4 |
Cargo genes
| Drug resistance gene | aadD, aac(6')-aph(2''), qacD, mupA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |