Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102998 |
| Name | oriT_pGO400  |
| Organism | Staphylococcus aureus subsp. aureus RN4220 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KT780705 (31214..31249 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pGO400
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2249 | GenBank | WP_001252101 |
| Name | MobA_MobL_HTG01_RS00150_pGO400 |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 1938 | GenBank | WP_000209436 |
| Name | t4cp2_HTG01_RS00070_pGO400 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3630..14851
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG01_RS00005 (pGO400_p35) | 41..1000 | + | 960 | WP_000043161 | replication initiator protein A | - |
| HTG01_RS00010 (pGO400_p01) | 1119..1793 | + | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| HTG01_RS00015 (pGO400_p02) | 1902..2090 | - | 189 | WP_001063224 | hypothetical protein | - |
| HTG01_RS00020 (pGO400_p03) | 2262..3236 | + | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| HTG01_RS00025 (pGO400_p04) | 3253..3570 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| HTG01_RS00030 (pGO400_p06) | 3630..4037 | + | 408 | WP_000110157 | hypothetical protein | trsC |
| HTG01_RS00035 (pGO400_p07) | 4024..4707 | + | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| HTG01_RS00040 (pGO400_p08) | 4722..6740 | + | 2019 | WP_001795400 | DUF87 domain-containing protein | virb4 |
| HTG01_RS00045 (pGO400_p09) | 6752..8032 | + | 1281 | WP_000702361 | membrane protein | trsF |
| HTG01_RS00050 (pGO400_p10) | 8050..9126 | + | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| HTG01_RS00055 (pGO400_p11) | 9136..9621 | + | 486 | WP_000735569 | TrsH/TraH family protein | - |
| HTG01_RS00060 (pGO400_p12) | 9637..11739 | + | 2103 | WP_001094109 | DNA topoisomerase III | - |
| HTG01_RS00065 (pGO400_p13) | 11755..12219 | + | 465 | WP_000715273 | hypothetical protein | trsJ |
| HTG01_RS00070 (pGO400_p14) | 12216..13856 | + | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HTG01_RS00075 (pGO400_p15) | 13934..14851 | + | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| HTG01_RS00080 (pGO400_p16) | 14868..15260 | + | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| HTG01_RS00085 (pGO400_p17) | 15317..15484 | + | 168 | WP_000869301 | hypothetical protein | - |
| HTG01_RS00090 (pGO400_p18) | 15522..16196 | + | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| HTG01_RS00095 (pGO400_p19) | 16414..19488 | + | 3075 | WP_047211916 | mupirocin-resistant isoleucine--tRNA ligase MupA | - |
Host bacterium
| ID | 3441 | GenBank | NZ_KT780705 |
| Plasmid name | pGO400 | Incompatibility group | - |
| Plasmid size | 34186 bp | Coordinate of oriT [Strand] | 31214..31249 [+] |
| Host baterium | Staphylococcus aureus subsp. aureus RN4220 |
Cargo genes
| Drug resistance gene | mupA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |