Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102994
Name   oriT_p203740_35 in_silico
Organism   Escherichia coli strain 203740
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP025915 (23983..24082 [+], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      32..38, 42..48  (TTACGAT..ATCGTAA)
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_p203740_35
AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAATCGTAATTGCGTATGGTGTATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2246 GenBank   WP_052953523
Name   TrwC_CXW72_RS25090_p203740_35 insolico UniProt ID   _
Length   968 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 968 a.a.        Molecular weight: 108373.52 Da        Isoelectric Point: 9.8255

>WP_052953523.1 MULTISPECIES: MobF family relaxase [Escherichia]
MLNITTITRQRVDRVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVSLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHELKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTRKQWVTELVKAGRSKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKSVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDTREKGRDKAEPTQDKGNARPKDKGHEKNNDDRALRL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(14-287)

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)


  Protein structure



No available structure.




T4CP

ID   1933 GenBank   WP_047929022
Name   t4cp2_CXW72_RS25095_p203740_35 insolico UniProt ID   _
Length   525 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 525 a.a.        Molecular weight: 58988.82 Da        Isoelectric Point: 9.4812

>WP_047929022.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MNEADKGKICLGALLFTPLVTWFISAKALYGFTPKDARERLVLLIQQTFDLWPLWGALLVGEIIAIIGLI
IFFKFNHQIFKGAPFKKIYRGTRLVSQRALASITKEREPQITIADIPIPTKAEGTHISIAGATGVGKSTI
FAEMMKCCLMRGKKQRIASNKDRMIILDPDGDFLSRFYKKGDKILNPYDARTEGWVFFNEIKSDFDFERF
GVSIVPTAKDSQTEEWNGYGRLLFTEVSRKVFNTSRNPTMEEVFYWTNEVPLEELEEYVRGTKAQALFAG
SGRAVGSARFVLSNKLAAHLKMPAGNFSIREWLEDPKGGNLYITWTDEMREALKSLISCWTDSIFSIVLG
MSKSNSRKIWTFIDELESLDYLPSLRDALTKGRKKGLRVVTGYQSYSQVISIYGSDVAETLLSNHRTSVV
MAAGRLGERTLEFVSKSLGEIEGEREKTGISRRFGQLGTKNTNDDHKRERAVTPTEIATLDDLTGYIAFP
GNLPVAKFETHHVNYTRSNPVPGVVLRESTAFAGM

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(111-499)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 6848..23541

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW72_RS24960 2876..3121 + 246 WP_033544576 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CXW72_RS24965 3121..3663 + 543 WP_047929020 hypothetical protein -
CXW72_RS24970 3633..3929 + 297 WP_226426392 hypothetical protein -
CXW72_RS24975 3919..4245 + 327 WP_000817647 endoribonuclease MazF -
CXW72_RS26155 4359..4760 - 402 WP_306415735 Hha/YmoA family nucleoid-associated regulatory protein -
CXW72_RS24985 4761..5486 - 726 WP_226426391 restriction endonuclease -
CXW72_RS24990 5462..5776 - 315 WP_033544579 hypothetical protein -
CXW72_RS24995 5780..6001 - 222 WP_226426390 TrbM/KikA/MpfK family conjugal transfer protein -
CXW72_RS25000 6100..6537 - 438 WP_033544581 hypothetical protein -
CXW72_RS25005 6552..6785 - 234 WP_033544582 H-NS histone family protein -
CXW72_RS25010 6848..7588 + 741 WP_033544583 lytic transglycosylase domain-containing protein virB1
CXW72_RS25015 7611..7928 + 318 WP_050008675 hypothetical protein -
CXW72_RS25020 7912..8202 + 291 WP_033544584 TrbC/VirB2 family protein virB2
CXW72_RS25025 8254..8568 + 315 WP_033544585 VirB3 family type IV secretion system protein virB3
CXW72_RS25030 8868..11198 + 2331 WP_226426389 VirB4 family type IV secretion/conjugal transfer ATPase -
CXW72_RS25035 11208..11915 + 708 WP_047929021 type IV secretion system protein virB5
CXW72_RS25040 11919..12155 + 237 WP_033544588 EexN family lipoprotein -
CXW72_RS25045 12210..13217 + 1008 WP_170913282 type IV secretion system protein virB6
CXW72_RS25430 13312..13482 + 171 WP_021536809 hypothetical protein -
CXW72_RS25050 13485..14201 + 717 WP_000914645 type IV secretion system protein virB8
CXW72_RS25055 14204..15091 + 888 WP_033544590 TrbG/VirB9 family P-type conjugative transfer protein virB9
CXW72_RS25060 15088..16242 + 1155 WP_050008676 type IV secretion system protein VirB10 virB10
CXW72_RS25065 16235..17266 + 1032 WP_033544592 P-type DNA transfer ATPase VirB11 virB11
CXW72_RS25070 17229..17783 + 555 WP_042036424 phospholipase D family protein -
CXW72_RS25075 17796..18278 + 483 WP_033544604 GNAT family protein -
CXW72_RS25080 18290..18484 + 195 WP_000529710 hypothetical protein -
CXW72_RS25085 18758..19045 - 288 WP_033544594 hypothetical protein -
CXW72_RS25090 19055..21961 - 2907 WP_052953523 MobF family relaxase -
CXW72_RS25095 21964..23541 - 1578 WP_047929022 type IV secretion system DNA-binding domain-containing protein virb4
CXW72_RS25100 23534..23881 - 348 WP_047929023 hypothetical protein -
CXW72_RS25105 24366..24800 + 435 WP_053273076 hypothetical protein -
CXW72_RS25110 24828..25523 + 696 WP_265737297 StbB family protein -
CXW72_RS25115 25525..25893 + 369 WP_015345231 plasmid stabilization protein StbC -
CXW72_RS25120 26107..26877 + 771 WP_039026342 SprT-like domain-containing protein -
CXW72_RS25435 26997..27152 - 156 WP_021536821 hypothetical protein -
CXW72_RS25125 27307..27549 - 243 WP_042036420 hypothetical protein -
CXW72_RS25130 27585..27872 - 288 WP_001098466 hypothetical protein -
CXW72_RS25135 28023..28244 + 222 WP_180305487 hypothetical protein -

Host bacterium

ID   3437 GenBank   NZ_CP025915
Plasmid name   p203740_35 Incompatibility group   -
Plasmid size   35753 bp Coordinate of oriT [Strand]   23983..24082 [+]
Host baterium   Escherichia coli strain 203740

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -