Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102991
• Name: oriT1_pRIVM1183
• Organism: Staphylococcus aureus strain RIVM1183
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP013627 ( 2865..2905 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT1_pRIVM1183
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_Pre_AUC55_RS00010_pRIVM1183

• ID: 2244
• GenBank: WP_032494831
• Name: Mob_Pre_AUC55_RS00010_pRIVM1183
• UniProt ID: _
• Length: 403 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 403 a.a.
• Molecular weight: 46964.92 Da
• Isoelectric Point: 9.2169

>WP_032494831.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSFVVARMQKMKSGNLVGVGNHNQRNTDNHSNKDIDVERSYLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNQFFKDKDDKEIKDFFEQSKDYFAEKFGDENIRYAQVHLDETTPHMHLGIVPFN
AEKKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEMTTTLEQRKS
EVLALSNDKTATIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIISKDKLNALIQGYE
DLYSSNERLKKYAETDLPKEITKVKGKYSELVKEYNDLVRKSNRNIETLEELEKENKSLKNEIKDIYKGF
KAYFKDSKQVTTEQVKTLVGGVVDKVKEFVKGGEFEKIHRNETRTRDRDELSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 3434
• GenBank: NZ_CP013627
• Plasmid name: pRIVM1183
• Incompatibility group: -
• Plasmid size: 6183 bp
• Coordinate of oriT [Strand]: 4137..4177 [-]; 2865..2905 [-]
• Host baterium: Staphylococcus aureus strain RIVM1183

Cargo genes

• Drug resistance gene: erm(T), tet(L)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -