Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102989
• Name: oriT_pRIVM4256
• Organism: Staphylococcus aureus strain RIVM4256
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP013629 (1808..1848 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pRIVM4256
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_Pre_AUC57_RS00010_pRIVM4256

• ID: 2243
• GenBank: WP_023296837
• Name: Mob_Pre_AUC57_RS00010_pRIVM4256
• UniProt ID: A0A2A4H9S9
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49704.29 Da
• Isoelectric Point: 9.8815

>WP_023296837.1 MULTISPECIES: MobV family relaxase [Bacillales]
MSYAVCRMQKVKSPGLKGMQFHNQRERESRTNEDIDRERTHENYDLQNDKNINYNERVKDIIDSQKTGTR
KTRKDAVLVNELLVTSDRDFFERLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETSKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKNVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAVRDN
DKLKQHVSNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTTQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: A0A2A4H9S9

Host bacterium

• ID: 3432
• GenBank: NZ_CP013629
• Plasmid name: pRIVM4256
• Incompatibility group: -
• Plasmid size: 7320 bp
• Coordinate of oriT [Strand]: 1808..1848 [-]
• Host baterium: Staphylococcus aureus strain RIVM4256

Cargo genes

• Drug resistance gene: dfrK, tet(L), aadD
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -