Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102988
• Name: oriT_pRIVM4390
• Organism: Staphylococcus aureus strain RIVM4390
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP013623 (2257..2354 [+], 98 nt)
• oriT length: 98 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 98 nt

>oriT_pRIVM4390
TCGGCAGAAACTTTGAATATGACAGTGCCTAATTTCGTTAAGAAAAAGGCACAAGGCAGTCGATTAGTGTCGCCAAAATTAGACAAAGAAACGCGACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_AUC51_RS00005_pRIVM4390

• ID: 2242
• GenBank: WP_031915289
• Name: Rep_trans_AUC51_RS00005_pRIVM4390
• UniProt ID: A0A0U2YLU7
• Length: 314 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 314 a.a.
• Molecular weight: 37865.14 Da
• Isoelectric Point: 7.4627

>WP_031915289.1 replication initiation factor domain-containing protein [Staphylococcus aureus]
MSKKAEEIQAKQSLEKENSNFSKTGYSNSRLGVHSVHPQNPKLSFDAMTIVGNLSRDNAQKLSSFMSIEP
QIRLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTR
LDLAFDFEDDLSDYYAMTDKAVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADVEVMSEHL
WRVEIELKRDMVDYWNDCFNDLHILKPDWTSPEKLNEQAMVYMLIHEEGKWGELNKRTKYKYKKIIKEIS
PIDLTEIMKLTLRENEKQLQKQIEFWQREFRFWE

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (138-230)

Protein structure

• AlphaFold DB: A0A0U2YLU7

Host bacterium

• ID: 3431
• GenBank: NZ_CP013623
• Plasmid name: pRIVM4390
• Incompatibility group: -
• Plasmid size: 4397 bp
• Coordinate of oriT [Strand]: 2257..2354 [+]
• Host baterium: Staphylococcus aureus strain RIVM4390

Cargo genes

• Drug resistance gene: str
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -