Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102985 |
| Name | oriT_PU25001  |
| Organism | Klebsiella pneumoniae strain U25 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KT203286 (104243..104292 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_PU25001
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2239 | GenBank | WP_043907038 |
| Name | traI_HTF68_RS00310_PU25001 |
UniProt ID | A0A0S1TKY9 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190458.19 Da Isoelectric Point: 6.0263
>WP_043907038.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSLSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTELTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSLSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTELTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1930 | GenBank | WP_043907039 |
| Name | traD_HTF68_RS00315_PU25001 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85906.85 Da Isoelectric Point: 5.0577
>WP_043907039.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 75686..104850
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTF68_RS00315 | 75686..77998 | - | 2313 | WP_043907039 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HTF68_RS00320 | 78127..78816 | - | 690 | WP_004198206 | hypothetical protein | - |
| HTF68_RS00325 | 79008..79739 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| HTF68_RS00330 | 79923..80471 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| HTF68_RS00335 | 80484..83333 | - | 2850 | WP_004152624 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HTF68_RS00340 | 83333..84712 | - | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| HTF68_RS00345 | 84690..85133 | - | 444 | WP_030003485 | F-type conjugal transfer protein TrbF | - |
| HTF68_RS00350 | 85179..85736 | - | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HTF68_RS00355 | 85708..85947 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HTF68_RS00360 | 85958..86710 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HTF68_RS00365 | 86731..87057 | - | 327 | WP_013214030 | hypothetical protein | - |
| HTF68_RS00370 | 87070..87318 | - | 249 | WP_013214029 | hypothetical protein | - |
| HTF68_RS00375 | 87296..87550 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| HTF68_RS00380 | 87582..89537 | - | 1956 | WP_040145764 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HTF68_RS00385 | 89585..90223 | - | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HTF68_RS00390 | 90236..91195 | - | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| HTF68_RS00395 | 91239..91865 | - | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| HTF68_RS00400 | 91865..92243 | - | 379 | Protein_81 | type-F conjugative transfer system protein TrbI | - |
| HTF68_RS00405 | 92243..94882 | - | 2640 | WP_004152592 | type IV secretion system protein TraC | virb4 |
| HTF68_RS00410 | 94954..95352 | - | 399 | WP_004153071 | hypothetical protein | - |
| HTF68_RS00415 | 95648..96052 | - | 405 | WP_004152594 | hypothetical protein | - |
| HTF68_RS00420 | 96119..96436 | - | 318 | WP_004152595 | hypothetical protein | - |
| HTF68_RS00425 | 96437..96655 | - | 219 | WP_004171484 | hypothetical protein | - |
| HTF68_RS00430 | 96679..96969 | - | 291 | WP_004152596 | hypothetical protein | - |
| HTF68_RS00435 | 96974..97384 | - | 411 | WP_004152597 | lipase chaperone | - |
| HTF68_RS00440 | 97516..98085 | - | 570 | WP_004152598 | type IV conjugative transfer system lipoprotein TraV | traV |
| HTF68_RS00445 | 98108..98704 | - | 597 | WP_004152599 | conjugal transfer pilus-stabilizing protein TraP | - |
| HTF68_RS00450 | 98697..100121 | - | 1425 | WP_004152600 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HTF68_RS00455 | 100121..100861 | - | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| HTF68_RS00460 | 100848..101414 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| HTF68_RS00465 | 101434..101739 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| HTF68_RS00470 | 101753..102121 | - | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| HTF68_RS00475 | 102175..102546 | - | 372 | WP_004208838 | TraY domain-containing protein | - |
| HTF68_RS00480 | 102630..103316 | - | 687 | WP_011977735 | transcriptional regulator TraJ family protein | - |
| HTF68_RS00485 | 103539..103931 | - | 393 | WP_004178062 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HTF68_RS00490 | 104365..104850 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| HTF68_RS00495 | 104883..105212 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| HTF68_RS00500 | 105245..106066 | - | 822 | WP_004178064 | DUF932 domain-containing protein | - |
| HTF68_RS00505 | 106900..107313 | - | 414 | WP_004152722 | helix-turn-helix domain-containing protein | - |
| HTF68_RS00510 | 107314..107592 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| HTF68_RS00515 | 107582..107902 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTF68_RS00520 | 107983..108198 | - | 216 | WP_077268719 | hypothetical protein | - |
| HTF68_RS00525 | 108218..108430 | - | 213 | WP_004152718 | hypothetical protein | - |
| HTF68_RS00530 | 108491..108847 | - | 357 | WP_004152717 | hypothetical protein | - |
| HTF68_RS00535 | 109483..109833 | - | 351 | WP_004152758 | hypothetical protein | - |
Host bacterium
| ID | 3428 | GenBank | NZ_KT203286 |
| Plasmid name | PU25001 | Incompatibility group | IncFII |
| Plasmid size | 211813 bp | Coordinate of oriT [Strand] | 104243..104292 [+] |
| Host baterium | Klebsiella pneumoniae strain U25 |
Cargo genes
| Drug resistance gene | catA1 |
| Virulence gene | - |
| Metal resistance gene | fecD, fecE, silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |