Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102960
Name   oriT_pEh1A in_silico
Organism   Enterobacter hormaechei strain E0083033-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KR822246 (80029..80110 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pEh1A
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2218 GenBank   WP_015632446
Name   Relaxase_HTF65_RS00400_pEh1A insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   1906 GenBank   WP_015632442
Name   trbC_HTF65_RS00365_pEh1A insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50327..72168

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTF65_RS00230 (AA409_0040) 45880..46266 - 387 WP_015632417 acyl-CoA thioesterase -
HTF65_RS00235 (AA409_0041) 46266..46751 - 486 WP_015632418 hypothetical protein -
HTF65_RS00240 (AA409_0042) 46718..47296 - 579 WP_015632419 hypothetical protein -
HTF65_RS00245 (AA409_0043) 47564..48097 + 534 WP_015632420 transcription termination/antitermination NusG family protein -
HTF65_RS00250 (AA409_0044) 48115..48318 + 204 WP_032495743 hypothetical protein -
HTF65_RS00255 (AA409_0045) 48352..48663 + 312 WP_015632421 hypothetical protein -
HTF65_RS00260 (AA409_0047) 49361..49840 + 480 WP_015632423 lytic transglycosylase domain-containing protein -
HTF65_RS00265 (AA409_0048) 49863..50330 + 468 WP_015632424 DotD/TraH family lipoprotein -
HTF65_RS00270 (AA409_0049) 50327..51145 + 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
HTF65_RS00275 (AA409_0050) 51123..52310 + 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
HTF65_RS00280 (AA409_0051) 52307..52573 + 267 WP_032441926 IcmT/TraK family protein traK
HTF65_RS00285 (AA409_0052) 52591..55914 + 3324 WP_032495745 LPD7 domain-containing protein -
HTF65_RS00290 (AA409_0053) 55948..56301 + 354 WP_015632427 hypothetical protein traL
HTF65_RS00295 (AA409_0054) 56337..57041 + 705 WP_015632428 DotI/IcmL/TraM family protein traM
HTF65_RS00300 (AA409_0055) 57052..58155 + 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
HTF65_RS00305 (AA409_0056) 58159..59499 + 1341 WP_015632430 conjugal transfer protein TraO traO
HTF65_RS00310 (AA409_0057) 59505..60224 + 720 WP_015632431 conjugal transfer protein TraP traP
HTF65_RS00315 (AA409_0058) 60234..60767 + 534 WP_015632432 conjugal transfer protein TraQ traQ
HTF65_RS00320 (AA409_0059) 60821..61222 + 402 WP_015632433 DUF6750 family protein traR
HTF65_RS00325 (AA409_0060) 61266..61829 + 564 WP_032495746 hypothetical protein traT
HTF65_RS00330 (AA409_0061) 61848..64886 + 3039 WP_335904770 ATP-binding protein -
HTF65_RS00335 (AA409_0062) 64933..66093 + 1161 WP_032495747 conjugal transfer protein TraW traW
HTF65_RS00340 (AA409_0063) 66090..66671 + 582 WP_015632437 conjugal transfer protein TraX -
HTF65_RS00345 (AA409_0064) 66713..68857 + 2145 WP_015632438 DotA/TraY family protein traY
HTF65_RS00350 (AA409_0065) 68923..69546 + 624 WP_015632439 plasmid IncI1-type surface exclusion protein ExcA -
HTF65_RS00355 (AA409_0066) 69648..71066 + 1419 WP_015632440 hypothetical protein trbA
HTF65_RS00360 (AA409_0067) 71014..72168 + 1155 WP_015632441 thioredoxin fold domain-containing protein trbB
HTF65_RS00365 (AA409_0068) 72170..74362 + 2193 WP_015632442 F-type conjugative transfer protein TrbC -
HTF65_RS00370 74408..74713 + 306 WP_015632443 phospholipase D-like domain-containing protein -
HTF65_RS00375 (AA409_0069) 74736..76325 - 1590 WP_015632444 IS66 family transposase -
HTF65_RS00380 (AA409_0070) 76355..76704 - 350 Protein_76 IS66 family insertion sequence element accessory protein TnpB -
HTF65_RS00385 (AA409_0071) 76701..77108 - 408 WP_015632445 transposase -

Host bacterium

ID   3403 GenBank   NZ_KR822246
Plasmid name   pEh1A Incompatibility group   IncFII
Plasmid size   96124 bp Coordinate of oriT [Strand]   80029..80110 [-]
Host baterium   Enterobacter hormaechei strain E0083033-1

Cargo genes

Drug resistance gene   blaNDM-1
Virulence gene   -
Metal resistance gene   arsR, arsD, arsA, arsB, arsC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -