Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102956
Name   oriT_pIMP-HB623 in_silico
Organism   Enterobacter cloacae strain CRE623
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KM877517 (110447..110552 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_pIMP-HB623
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2215 GenBank   WP_050868819
Name   Relaxase_HTE41_RS00705_pIMP-HB623 insolico UniProt ID   A0A142BMF1
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75234.98 Da        Isoelectric Point: 10.0285

>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A142BMF1


T4CP

ID   1901 GenBank   WP_011091092
Name   t4cp2_HTE41_RS00435_pIMP-HB623 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79491.81 Da        Isoelectric Point: 4.8270

>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 113348..132496

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTE41_RS00685 108432..108743 + 312 WP_011091065 hypothetical protein -
HTE41_RS00690 108876..109412 + 537 WP_011091066 hypothetical protein -
HTE41_RS00695 109913..110278 - 366 WP_011091067 hypothetical protein -
HTE41_RS00830 110293..110871 + 579 WP_223811519 plasmid mobilization protein MobA -
HTE41_RS00705 110858..112837 + 1980 WP_050868819 TraI/MobA(P) family conjugative relaxase -
HTE41_RS00710 112851..113351 + 501 WP_004187320 DotD/TraH family lipoprotein -
HTE41_RS00715 113348..114127 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HTE41_RS00720 114138..115301 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
HTE41_RS00725 115291..115551 + 261 WP_004187310 IcmT/TraK family protein traK
HTE41_RS00835 115576..118833 + 3258 WP_222859430 LPD7 domain-containing protein -
HTE41_RS00735 118799..119311 + 513 WP_011091071 hypothetical protein traL
HTE41_RS00740 119312..119524 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
HTE41_RS00745 119496..119906 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTE41_RS00750 119974..120687 + 714 WP_109914255 DotI/IcmL family type IV secretion protein traM
HTE41_RS00755 120696..121847 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
HTE41_RS00760 121859..123208 + 1350 WP_011091075 conjugal transfer protein TraO traO
HTE41_RS00765 123220..123924 + 705 WP_172686522 conjugal transfer protein TraP traP
HTE41_RS00770 123948..124478 + 531 WP_011091077 conjugal transfer protein TraQ traQ
HTE41_RS00775 124495..124884 + 390 WP_011091078 DUF6750 family protein traR
HTE41_RS00780 124930..125424 + 495 WP_011091080 hypothetical protein -
HTE41_RS00785 125421..128471 + 3051 WP_011091081 hypothetical protein traU
HTE41_RS00790 128468..129676 + 1209 WP_011091082 conjugal transfer protein TraW traW
HTE41_RS00795 129673..130323 + 651 WP_011091083 hypothetical protein -
HTE41_RS00800 130316..132496 + 2181 WP_004187492 DotA/TraY family protein traY
HTE41_RS00805 132499..133152 + 654 WP_011091084 hypothetical protein -

Host bacterium

ID   3399 GenBank   NZ_KM877517
Plasmid name   pIMP-HB623 Incompatibility group   IncL/M
Plasmid size   133208 bp Coordinate of oriT [Strand]   110447..110552 [+]
Host baterium   Enterobacter cloacae strain CRE623

Cargo genes

Drug resistance gene   fosC2, blaIMP-34, aac(3)-IId, aph(3'')-Ib, aph(6)-Id, dfrA14, mph(A), blaTEM-1B
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -