Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102945
Name   oriT_pAsa4c in_silico
Organism   Aeromonas salmonicida subsp. salmonicida strain JF2267
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KT033470 (41630..41759 [+], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_pAsa4c
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCAGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2210 GenBank   WP_232048270
Name   TraI_2_HTG22_RS00200_pAsa4c insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 107581.92 Da        Isoelectric Point: 4.7871

>WP_232048270.1 MULTISPECIES: MobH family relaxase [Aeromonas]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKNQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTK
PAEGTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEK
AIQMLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKL
YLEVDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(22-328)


  Protein structure



No available structure.




T4CP

ID   1891 GenBank   WP_011899407
Name   traD_HTG22_RS00205_pAsa4c insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.



ID   1892 GenBank   WP_081374711
Name   traC_HTG22_RS00270_pAsa4c insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 91612.68 Da        Isoelectric Point: 5.8656

>WP_081374711.1 MULTISPECIES: type IV secretion system protein TraC [Aeromonas]
MIVTIKKKLDGALIQEHMRASGVIPVLAYSEDEQVFLMDDQSAGFGFICEPMCGADEKVQERMNGFLNQE
FPAKTTLQFLLFRSPDINQEMYQMLGLRDGFRHGLLSPVIQERINYLQQHTTERIMARTNKGIYDNGLIQ
DLKLFVTCKVPIKSNTPTETELVELDQLKTKVESSLQTVGLRPRTMTAVNYVRIMSTLLNWGPDASWRHD
ASRWETDKPICEQLFDYGTDLEVSKNGIRLGGYHAKVLSAKKLPDIAYFGDAVAYTGDLTGGNSSIKENY
MVVTNVFFPEVESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTVYESLKEGAKPVKISYSLVIF
APTKERVESAATAARSIWRESRFELMEDRFVALPIFLNSLPFCTDRDAVRDLFRYKTMTSEQAAVVLPVF
GEWKGTGTFHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELICSYLSEGAQVWVIDAGKS
YQKMSEMLRGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASSKGLLVEWQISALKQVLSSIWE
EKGTTMKVDDIAERCLKGEDLRLKDVGQQLYAFTSEGSYGKYFSNKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRAGRLALSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVNQGLSVPDAIKAVMQARRQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-808)

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 39709..63462

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTG22_RS00190 35682..35897 - 216 WP_043774467 hypothetical protein -
HTG22_RS00195 35879..36493 - 615 WP_073536823 hypothetical protein -
HTG22_RS00200 36743..39706 + 2964 WP_232048270 MobH family relaxase -
HTG22_RS00205 39709..41574 + 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
HTG22_RS00210 41585..42169 + 585 WP_073536825 hypothetical protein -
HTG22_RS00215 42126..42755 + 630 WP_073536826 DUF4400 domain-containing protein tfc7
HTG22_RS00220 42892..43290 + 399 WP_236762775 hypothetical protein -
HTG22_RS00225 43476..43628 + 153 WP_172686310 hypothetical protein -
HTG22_RS00230 43819..44100 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
HTG22_RS00235 44097..44732 + 636 WP_073536827 TraE/TraK family type IV conjugative transfer system protein traE
HTG22_RS00240 44716..45621 + 906 WP_059119365 type-F conjugative transfer system secretin TraK traK
HTG22_RS00245 45624..46943 + 1320 WP_088822001 TraB/VirB10 family protein traB
HTG22_RS00250 46940..47506 + 567 WP_073536829 type IV conjugative transfer system lipoprotein TraV -
HTG22_RS00255 47597..47923 + 327 WP_050804919 TraA family conjugative transfer protein -
HTG22_RS00260 48105..53591 + 5487 WP_073536830 Ig-like domain-containing protein -
HTG22_RS00265 53748..54473 + 726 WP_232484092 DsbC family protein trbB
HTG22_RS00270 54470..56914 + 2445 WP_081374711 type IV secretion system protein TraC virb4
HTG22_RS00275 56934..57251 + 318 WP_073536868 hypothetical protein -
HTG22_RS00280 57248..57778 + 531 WP_073536831 S26 family signal peptidase -
HTG22_RS00285 57741..59006 + 1266 WP_073536832 TrbC family F-type conjugative pilus assembly protein traW
HTG22_RS00290 59003..59674 + 672 WP_073536833 EAL domain-containing protein -
HTG22_RS00295 59671..60678 + 1008 WP_073536834 TraU family protein traU
HTG22_RS00300 60688..63462 + 2775 WP_073536835 conjugal transfer mating pair stabilization protein TraN traN
HTG22_RS00305 64523..65386 - 864 WP_073536836 hypothetical protein -
HTG22_RS00310 65531..66199 - 669 WP_081375035 hypothetical protein -
HTG22_RS00315 66488..66850 + 363 WP_073536838 hypothetical protein -
HTG22_RS00320 67142..67321 + 180 WP_073536839 hypothetical protein -

Host bacterium

ID   3388 GenBank   NZ_KT033470
Plasmid name   pAsa4c Incompatibility group   -
Plasmid size   163022 bp Coordinate of oriT [Strand]   41630..41759 [+]
Host baterium   Aeromonas salmonicida subsp. salmonicida strain JF2267

Cargo genes

Drug resistance gene   cat, ant(3'')-Ia, qacE, sul1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -