Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102944
Name   oriT_pAsa4b in_silico
Organism   Aeromonas salmonicida subsp. salmonicida strain 01-B522
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KT033469 (57965..58094 [+], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_pAsa4b
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2209 GenBank   WP_043774463
Name   TraI_2_HTG10_RS00280_pAsa4b insolico UniProt ID   A0A189PG74
Length   1013 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1013 a.a.        Molecular weight: 110403.21 Da        Isoelectric Point: 4.8427

>WP_043774463.1 MobH family relaxase [Aeromonas salmonicida]
MLSVLNKLFGWGGSKGESPQSGRLLPLKDMDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGF
TIEDFNRLVLPVIHRYAAFVHLLPASEAHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNE
PRWRLASCFSGLLHDVGKPLADVSITDKNGVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSL
LAVDRIIPVATREYLSESGPLIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGDRAYYRYWEVLPEMIQEAAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIE
LIDPDEASDADDESASGMSESLLSAEQEAMNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPA
KGAGGRGGKNQAKKTAPAAPEQQQPEPSPQEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTS
MSDEPSQALAPEPKQDVANPDNPDTPNLGLMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGE
VGQPGNQFVSEELAGADAPDAKGELLTLMATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPE
GARALGAPSEILTALFHAQAIVPDPVMSGRKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFE
LVASPEVKKTQSKKNRGQRKTSRSESSGEDSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVDTTPPP
DKVVPRKESRGKEKVDKPIQPAPTTKPTEGTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEE
LSPRSIVKEETVDEALYLPPKMTPEKAIQMLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTG
ISKHVLGGTLRRAQRRPLLKQRQGKLYLEVDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A189PG74


T4CP

ID   1889 GenBank   WP_011899407
Name   traD_HTG10_RS00285_pAsa4b insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.



ID   1890 GenBank   WP_081374866
Name   traC_HTG10_RS00385_pAsa4b insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 91684.72 Da        Isoelectric Point: 5.7286

>WP_081374866.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLDGALIQEHMRASGVIPVLAYSEDEQVFLMDDQSAGFGFICEPMCGADEKVQERMNGFLNQE
FPSKTTLQFLLFRSPDINQEMYQMLGLRDGFRHGLLSPVIQERINYLQHHTTERIMARTNKGIYDNGLIQ
DLKLFVTCKVPIKSNTPTETELVELDQLKTKIESSLQTVGLRPRTMTAVNYVRIMSTLLNWGPDASWRHD
ASRWETDKPICEQLFDYGTDLEVSKNGIRLGGYHAKVLSAKKLPDIAYFGDAVAYTGDLTGGNSSIKENY
MVVTNVFFPEVESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTVYESLKEGAKPVKISYSLVIF
APTKERVESAATAARSIWRESRFELMEDRFVALPIFLNSLPFCTDRDAVRDLFRYKTMTSEQAAVLLPVF
GEWKGTGTFHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELICSYLSEGAQVWVIDAGKS
YQKMSEMLRGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASSKGLLVEWQISALKQVMSSIWE
EKGTTMKVDDIAERCLEGEDLRLKDVGQQLYAFTSEGSYGKYFSNKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRAGRLALSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVNQGLSVPDAIKAVMQARRQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-808)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56044..83771

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTG10_RS00270 52026..52241 - 216 WP_043774467 hypothetical protein -
HTG10_RS00275 52223..52837 - 615 WP_011899409 hypothetical protein -
HTG10_RS00280 53000..56041 + 3042 WP_043774463 MobH family relaxase -
HTG10_RS00285 56044..57909 + 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
HTG10_RS00290 57920..58504 + 585 WP_011899406 hypothetical protein -
HTG10_RS00295 58461..59090 + 630 WP_011899405 DUF4400 domain-containing protein tfc7
HTG10_RS00300 59254..59628 + 375 WP_172686281 hypothetical protein -
HTG10_RS00305 59764..60333 + 570 WP_236762728 hypothetical protein -
HTG10_RS00985 60771..61199 + 429 WP_011899394 hypothetical protein -
HTG10_RS00315 61211..61438 + 228 WP_011899395 hypothetical protein -
HTG10_RS00320 61454..62068 + 615 WP_011899396 hypothetical protein -
HTG10_RS00325 62117..62362 + 246 WP_011899397 hypothetical protein -
HTG10_RS00335 62903..63184 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
HTG10_RS00340 63181..63816 + 636 WP_011899399 TraE/TraK family type IV conjugative transfer system protein traE
HTG10_RS00345 63800..64705 + 906 WP_059119365 type-F conjugative transfer system secretin TraK traK
HTG10_RS00350 64708..66027 + 1320 WP_139027009 TraB/VirB10 family protein traB
HTG10_RS00355 66120..66590 + 471 WP_236762731 type IV conjugative transfer system lipoprotein TraV traV
HTG10_RS00360 66681..67007 + 327 WP_050804919 TraA family conjugative transfer protein -
HTG10_RS00365 67189..68988 + 1800 WP_139027006 DUF4165 domain-containing protein -
HTG10_RS00370 68993..70090 - 1098 WP_010676219 ISAs1-like element ISAs1 family transposase -
HTG10_RS00375 70201..73896 + 3696 Protein_75 Ig-like domain-containing protein -
HTG10_RS00380 74052..74777 + 726 WP_223936722 DsbC family protein trbB
HTG10_RS00385 74774..77218 + 2445 WP_081374866 type IV secretion system protein TraC virb4
HTG10_RS00390 77243..77560 + 318 WP_073536868 hypothetical protein -
HTG10_RS00395 77557..78087 + 531 WP_073545150 S26 family signal peptidase -
HTG10_RS00400 78050..79315 + 1266 WP_073545151 TrbC family F-type conjugative pilus assembly protein traW
HTG10_RS00405 79312..79983 + 672 WP_073545152 EAL domain-containing protein -
HTG10_RS00410 79980..80987 + 1008 WP_073545153 TraU family protein traU
HTG10_RS00415 80997..83771 + 2775 WP_073545154 conjugal transfer mating pair stabilization protein TraN traN
HTG10_RS00420 84853..87276 - 2424 WP_172686283 DUF4238 domain-containing protein -
HTG10_RS00425 87519..87818 + 300 WP_172686284 hypothetical protein -
HTG10_RS00430 87833..88135 + 303 WP_073545160 hypothetical protein -

Host bacterium

ID   3387 GenBank   NZ_KT033469
Plasmid name   pAsa4b Incompatibility group   -
Plasmid size   181933 bp Coordinate of oriT [Strand]   57965..58094 [+]
Host baterium   Aeromonas salmonicida subsp. salmonicida strain 01-B522

Cargo genes

Drug resistance gene   tet(E), qacE, sul1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -