Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102942 |
| Name | oriT_p13C1065T-5  |
| Organism | Escherichia coli strain 13C1065T |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP019264 (28276..28328 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p13C1065T-5
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1888 | GenBank | WP_000338974 |
| Name | t4cp2_BWI86_RS27985_p13C1065T-5 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73347.89 Da Isoelectric Point: 9.1391
>WP_000338974.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 49871..73416
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BWI86_RS27850 (BWI86_27885) | 45895..46347 | + | 453 | WP_000101553 | CaiF/GrlA family transcriptional regulator | - |
| BWI86_RS27855 (BWI86_27890) | 46340..46534 | + | 195 | WP_000049865 | DUF1187 family protein | - |
| BWI86_RS27860 (BWI86_27895) | 46580..47533 | + | 954 | WP_072105959 | SPFH domain-containing protein | - |
| BWI86_RS27865 (BWI86_27900) | 47606..48049 | + | 444 | WP_000964840 | NfeD family protein | - |
| BWI86_RS29295 | 48053..48223 | + | 171 | WP_000550721 | hypothetical protein | - |
| BWI86_RS27870 (BWI86_27905) | 48234..48896 | + | 663 | WP_001419740 | hypothetical protein | - |
| BWI86_RS27880 (BWI86_27915) | 49080..49295 | + | 216 | WP_001360344 | hypothetical protein | - |
| BWI86_RS27885 (BWI86_27920) | 49311..49613 | + | 303 | WP_001360345 | hypothetical protein | - |
| BWI86_RS27890 (BWI86_27925) | 49610..49867 | + | 258 | WP_000739144 | hypothetical protein | - |
| BWI86_RS27895 (BWI86_27930) | 49871..50857 | + | 987 | WP_001419739 | type IV secretion system protein | virB6 |
| BWI86_RS27900 (BWI86_27935) | 50863..51510 | + | 648 | WP_001419738 | type IV secretion system protein | - |
| BWI86_RS27905 (BWI86_27940) | 51514..51750 | + | 237 | WP_000750964 | EexN family lipoprotein | - |
| BWI86_RS27910 (BWI86_27945) | 51797..52606 | - | 810 | WP_001419737 | DUF5710 domain-containing protein | - |
| BWI86_RS27915 (BWI86_27950) | 52654..53286 | + | 633 | WP_001419736 | hypothetical protein | - |
| BWI86_RS27920 (BWI86_27955) | 53353..53652 | + | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BWI86_RS27925 (BWI86_27960) | 53655..54890 | + | 1236 | WP_001419735 | TcpQ domain-containing protein | - |
| BWI86_RS27930 (BWI86_27965) | 54896..55333 | + | 438 | WP_015387358 | type IV pilus biogenesis protein PilM | - |
| BWI86_RS27935 (BWI86_27970) | 55673..56071 | + | 399 | WP_001708012 | hypothetical protein | - |
| BWI86_RS27940 (BWI86_27975) | 56092..56676 | + | 585 | WP_001401693 | lytic transglycosylase domain-containing protein | virB1 |
| BWI86_RS29785 | 56676..56966 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| BWI86_RS27950 (BWI86_27985) | 57037..57357 | + | 321 | WP_000362081 | VirB3 family type IV secretion system protein | virB3 |
| BWI86_RS27955 (BWI86_27990) | 57363..59720 | + | 2358 | WP_148116478 | VirB4 family type IV secretion system protein | virb4 |
| BWI86_RS27965 (BWI86_28000) | 59884..60618 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BWI86_RS27970 (BWI86_28005) | 60684..61385 | + | 702 | WP_053882504 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BWI86_RS27975 (BWI86_28010) | 61375..62514 | + | 1140 | WP_015387354 | TrbI/VirB10 family protein | virB10 |
| BWI86_RS27980 (BWI86_28015) | 62533..63588 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BWI86_RS27985 (BWI86_28020) | 63604..65562 | + | 1959 | WP_000338974 | type IV secretory system conjugative DNA transfer family protein | - |
| BWI86_RS27990 (BWI86_28025) | 65609..66127 | + | 519 | WP_015387353 | YfdA protein | virb4 |
| BWI86_RS27995 (BWI86_28030) | 66120..67763 | + | 1644 | WP_001035588 | PilN family type IVB pilus formation outer membrane protein | - |
| BWI86_RS28000 (BWI86_28035) | 67814..69124 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BWI86_RS28005 (BWI86_28040) | 69108..69602 | + | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BWI86_RS28010 (BWI86_28045) | 69627..71165 | + | 1539 | WP_000466227 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BWI86_RS28015 (BWI86_28050) | 71156..72265 | + | 1110 | WP_000974902 | type II secretion system F family protein | - |
| BWI86_RS28020 (BWI86_28055) | 72310..72867 | + | 558 | WP_000095049 | type 4 pilus major pilin | - |
| BWI86_RS28025 (BWI86_28060) | 72934..73416 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BWI86_RS28030 (BWI86_28065) | 73420..74055 | + | 636 | WP_000934978 | A24 family peptidase | - |
| BWI86_RS28035 (BWI86_28070) | 74068..75384 | + | 1317 | WP_074526577 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BWI86_RS28040 (BWI86_28075) | 75363..75716 | - | 354 | WP_157909760 | pilus assembly protein | - |
| BWI86_RS28050 (BWI86_28090) | 76293..76448 | + | 156 | WP_001358489 | hypothetical protein | - |
| BWI86_RS30175 (BWI86_28105) | 77069..77290 | - | 222 | Protein_93 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 3385 | GenBank | NZ_CP019264 |
| Plasmid name | p13C1065T-5 | Incompatibility group | IncI2 |
| Plasmid size | 82265 bp | Coordinate of oriT [Strand] | 28276..28328 [+] |
| Host baterium | Escherichia coli strain 13C1065T |
Cargo genes
| Drug resistance gene | mcr-1.1, blaCTX-M-55 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |