Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102939
Name   oriT_p13C1065T-2 in_silico
Organism   Escherichia coli strain 13C1065T
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP019261 (62523..62874 [-], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      255..262, 271..278  (ACCGCTAG..CTAGCGGT)
 192..198, 206..212  (TATAAAA..TTTTATA)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_p13C1065T-2
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGAGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGCTACAACAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCTAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCATTATGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTCAAAAAAGGTAATCTCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2206 GenBank   WP_054377139
Name   traI_BWI86_RS25805_p13C1065T-2 insolico UniProt ID   A0A5W1N632
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191884.75 Da        Isoelectric Point: 6.1058

>WP_054377139.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHTVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNSYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGEILLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTDLGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESGRPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSGRQQTRVIRPAQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETVRRADEIVRKMAENKPDLP
DSKTEQAVREIAGQERDRSAISEREAALPESVLRESQREREAVREIARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




Auxiliary protein

ID   907 GenBank   WP_001369361
Name   WP_001369361_p13C1065T-2 insolico UniProt ID   B7LI64
Length   133 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 133 a.a.        Molecular weight: 15409.70 Da        Isoelectric Point: 9.9240

>WP_001369361.1 MULTISPECIES: conjugal transfer relaxosome DNA-bindin protein TraY [Gammaproteobacteria]
MLLKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFNTL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(18-61)

TraY domain (TraY)

(72-119)


  Protein structure


Source ID Structure
AlphaFold DB B7LI64

ID   908 GenBank   WP_001151524
Name   WP_001151524_p13C1065T-2 insolico UniProt ID   A0A5W2DT22
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14517.48 Da        Isoelectric Point: 5.0516

>WP_001151524.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A5W2DT22


T4CP

ID   1886 GenBank   WP_063078088
Name   traD_BWI86_RS25810_p13C1065T-2 insolico UniProt ID   _
Length   726 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 726 a.a.        Molecular weight: 82541.06 Da        Isoelectric Point: 5.0504

>WP_063078088.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ
PQQPVSSAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQ
QMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   1887 GenBank   WP_001064265
Name   traC_BWI86_RS25885_p13C1065T-2 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99296.07 Da        Isoelectric Point: 6.3032

>WP_001064265.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKNRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
AGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

Family of unknown function (DUF5934) (DUF5934)

(289-446)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 38186..63445

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS25810 (BWI86_25830) 38186..40366 - 2181 WP_063078088 type IV conjugative transfer system coupling protein TraD virb4
BWI86_RS25815 (BWI86_25835) 40619..41350 - 732 WP_000850424 conjugal transfer complement resistance protein TraT -
BWI86_RS25820 (BWI86_25840) 41382..41885 - 504 WP_000605510 hypothetical protein -
BWI86_RS25825 (BWI86_25845) 41900..44722 - 2823 WP_001007048 conjugal transfer mating-pair stabilization protein TraG traG
BWI86_RS25830 (BWI86_25850) 44719..46092 - 1374 WP_170926237 conjugal transfer pilus assembly protein TraH traH
BWI86_RS25835 (BWI86_25855) 46092..46454 - 363 WP_001443292 P-type conjugative transfer protein TrbJ -
BWI86_RS25840 (BWI86_25860) 46384..46929 - 546 WP_000052613 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
BWI86_RS25845 (BWI86_25865) 46916..47152 - 237 WP_054377141 type-F conjugative transfer system pilin chaperone TraQ -
BWI86_RS25850 (BWI86_25870) 47313..48056 - 744 WP_039002205 type-F conjugative transfer system pilin assembly protein TraF traF
BWI86_RS25855 (BWI86_25875) 48049..48309 - 261 WP_000864348 conjugal transfer protein TrbE -
BWI86_RS25860 (BWI86_25880) 48336..50144 - 1809 WP_000821844 type-F conjugative transfer system mating-pair stabilization protein TraN traN
BWI86_RS25865 (BWI86_25885) 50141..50779 - 639 WP_000777691 type-F conjugative transfer system pilin assembly protein TrbC trbC
BWI86_RS25870 (BWI86_25890) 50788..51780 - 993 WP_023148235 conjugal transfer pilus assembly protein TraU traU
BWI86_RS25875 (BWI86_25895) 51777..52409 - 633 WP_001749440 type-F conjugative transfer system protein TraW traW
BWI86_RS25880 (BWI86_25900) 52406..52792 - 387 WP_039002198 type-F conjugative transfer system protein TrbI -
BWI86_RS25885 (BWI86_25905) 52789..55416 - 2628 WP_001064265 type IV secretion system protein TraC virb4
BWI86_RS25890 (BWI86_25910) 55576..55797 - 222 WP_001278692 conjugal transfer protein TraR -
BWI86_RS25895 (BWI86_25915) 55932..56447 - 516 WP_000724019 type IV conjugative transfer system lipoprotein TraV traV
BWI86_RS25900 (BWI86_25920) 56444..56764 - 321 WP_063078087 conjugal transfer protein TrbD -
BWI86_RS25905 (BWI86_25925) 56751..57338 - 588 WP_000002772 conjugal transfer pilus-stabilizing protein TraP -
BWI86_RS25910 (BWI86_25930) 57328..58755 - 1428 WP_000146690 F-type conjugal transfer pilus assembly protein TraB traB
BWI86_RS25915 (BWI86_25935) 58755..59483 - 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
BWI86_RS25920 (BWI86_25940) 59470..60036 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
BWI86_RS25925 (BWI86_25945) 60058..60369 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
BWI86_RS25930 (BWI86_25950) 60374..60736 - 363 WP_000994784 type IV conjugative transfer system pilin TraA -
BWI86_RS25935 (BWI86_25955) 60769..61164 - 396 WP_001309237 conjugal transfer relaxosome DNA-bindin protein TraY -
BWI86_RS25940 (BWI86_25960) 61263..61952 - 690 WP_039002187 conjugal transfer transcriptional regulator TraJ -
BWI86_RS25945 (BWI86_25965) 62139..62522 - 384 WP_001151524 conjugal transfer relaxosome DNA-binding protein TraM -
BWI86_RS25950 (BWI86_25970) 62855..63445 + 591 WP_001376243 transglycosylase SLT domain-containing protein -
BWI86_RS25955 (BWI86_25975) 63742..64563 - 822 WP_072795862 DUF932 domain-containing protein -
BWI86_RS25960 (BWI86_25980) 64683..64970 - 288 WP_000107535 hypothetical protein -
BWI86_RS29650 64995..65201 - 207 WP_000547968 hypothetical protein -
BWI86_RS29655 (BWI86_25990) 65271..65444 + 174 Protein_76 hypothetical protein -
BWI86_RS29660 65442..65672 - 231 WP_001426396 hypothetical protein -
BWI86_RS25980 (BWI86_25995) 65892..66017 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
BWI86_RS29665 65959..66108 - 150 Protein_79 plasmid maintenance protein Mok -
BWI86_RS28840 66130..66318 + 189 WP_001299721 hypothetical protein -
BWI86_RS25985 (BWI86_26000) 66287..67049 - 763 Protein_81 plasmid SOS inhibition protein A -
BWI86_RS25990 (BWI86_26005) 67046..67480 - 435 WP_000845953 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   3382 GenBank   NZ_CP019261
Plasmid name   p13C1065T-2 Incompatibility group   IncFIC
Plasmid size   138610 bp Coordinate of oriT [Strand]   62523..62874 [-]
Host baterium   Escherichia coli strain 13C1065T

Cargo genes

Drug resistance gene   floR, sul2, aph(3')-Ia, sul3, blaTEM-1B, lnu(F), ant(3'')-Ia, aac(3)-IId, tet(M), cmlA1, aadA2, dfrA12
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -