Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102934 |
| Name | oriT1_p13P477T-4  |
| Organism | Escherichia coli strain 13P477T |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP019277 ( 6051..6103 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT1_p13P477T-4
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1882 | GenBank | WP_000338976 |
| Name | t4cp2_BWI89_RS27595_p13P477T-4 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73346.95 Da Isoelectric Point: 9.3476
>WP_000338976.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 1883 | GenBank | WP_236919631 |
| Name | t4cp2_BWI89_RS27995_p13P477T-4 |
UniProt ID | _ |
| Length | 522 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 522 a.a. Molecular weight: 58860.52 Da Isoelectric Point: 8.2960
>WP_236919631.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MFKGRYKKQFIYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKC
FLFAPAGYAETIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLG
LYLLDKERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSA
PDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVN
ENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTT
FTENSAVVLYYPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSL
RDKKNKARNIAIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIY
LPKAGGKKVMVAGGNVITNPDLDNHDKTDVSE
MFKGRYKKQFIYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKC
FLFAPAGYAETIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLG
LYLLDKERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSA
PDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVN
ENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTT
FTENSAVVLYYPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSL
RDKKNKARNIAIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIY
LPKAGGKKVMVAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26969..49793
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BWI89_RS27520 (BWI89_27500) | 22011..23543 | - | 1533 | WP_000998025 | IS66-like element ISEc8 family transposase | - |
| BWI89_RS27525 (BWI89_27505) | 23593..23940 | - | 348 | WP_000612591 | IS66 family insertion sequence element accessory protein TnpB | - |
| BWI89_RS27530 (BWI89_27510) | 23937..24317 | - | 381 | WP_001171554 | transposase | - |
| BWI89_RS29445 (BWI89_27515) | 24439..24699 | - | 261 | WP_198965019 | pilus assembly protein | - |
| BWI89_RS27545 (BWI89_27525) | 25076..26317 | - | 1242 | Protein_28 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BWI89_RS27550 (BWI89_27530) | 26330..26965 | - | 636 | WP_000934979 | A24 family peptidase | - |
| BWI89_RS27555 (BWI89_27535) | 26969..27451 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BWI89_RS27560 (BWI89_27540) | 27517..28074 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BWI89_RS27565 (BWI89_27545) | 28119..29228 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BWI89_RS27570 (BWI89_27550) | 29219..30757 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BWI89_RS27575 (BWI89_27555) | 30782..31276 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BWI89_RS27580 (BWI89_27560) | 31260..32570 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BWI89_RS27585 (BWI89_27565) | 32621..34264 | - | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| BWI89_RS27590 (BWI89_27570) | 34257..34799 | - | 543 | WP_001220544 | sigma 54-interacting transcriptional regulator | virb4 |
| BWI89_RS27595 (BWI89_27575) | 34846..36804 | - | 1959 | WP_000338976 | type IV secretory system conjugative DNA transfer family protein | - |
| BWI89_RS27600 (BWI89_27580) | 36820..37875 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| BWI89_RS27605 (BWI89_27585) | 37894..39033 | - | 1140 | WP_001542008 | TrbI/VirB10 family protein | virB10 |
| BWI89_RS27610 (BWI89_27590) | 39023..39724 | - | 702 | WP_049824867 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BWI89_RS27615 (BWI89_27595) | 39790..40524 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BWI89_RS27625 (BWI89_27605) | 40690..43047 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| BWI89_RS27630 (BWI89_27610) | 43053..43373 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BWI89_RS30245 | 43444..43734 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| BWI89_RS27640 (BWI89_27620) | 43734..44318 | - | 585 | WP_065358197 | lytic transglycosylase domain-containing protein | virB1 |
| BWI89_RS27645 (BWI89_27625) | 44339..44737 | - | 399 | WP_001153669 | hypothetical protein | - |
| BWI89_RS27650 (BWI89_27630) | 44856..45293 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| BWI89_RS27655 (BWI89_27635) | 45299..46534 | - | 1236 | WP_053899060 | toxin co-regulated pilus biosynthesis Q family protein | - |
| BWI89_RS27660 (BWI89_27640) | 46537..46836 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BWI89_RS30590 (BWI89_27645) | 46884..47692 | + | 809 | Protein_51 | DUF5710 domain-containing protein | - |
| BWI89_RS27670 (BWI89_27650) | 47915..48139 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| BWI89_RS27675 (BWI89_27655) | 48148..48792 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| BWI89_RS27680 (BWI89_27660) | 48798..49793 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| BWI89_RS27685 (BWI89_27665) | 49797..50054 | - | 258 | WP_000739144 | hypothetical protein | - |
| BWI89_RS27690 (BWI89_27670) | 50051..50353 | - | 303 | WP_001360345 | hypothetical protein | - |
| BWI89_RS27695 (BWI89_27675) | 50334..50591 | - | 258 | WP_001542015 | hypothetical protein | - |
| BWI89_RS27700 (BWI89_27680) | 50624..51070 | - | 447 | WP_001243165 | hypothetical protein | - |
| BWI89_RS29740 | 51081..51251 | - | 171 | WP_000550720 | hypothetical protein | - |
| BWI89_RS27705 (BWI89_27685) | 51255..51698 | - | 444 | WP_000964330 | NfeD family protein | - |
| BWI89_RS27715 (BWI89_27695) | 52072..53025 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BWI89_RS29745 | 53052..53228 | - | 177 | WP_000753050 | hypothetical protein | - |
| BWI89_RS27720 (BWI89_27700) | 53221..53436 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| BWI89_RS27725 (BWI89_27705) | 53429..53881 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Region 2: 89999..103557
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BWI89_RS29460 (BWI89_27910) | 85348..85608 | + | 261 | WP_198965019 | pilus assembly protein | - |
| BWI89_RS27930 (BWI89_27915) | 85730..86110 | + | 381 | WP_001171554 | transposase | - |
| BWI89_RS27935 (BWI89_27920) | 86107..86454 | + | 348 | WP_000612591 | IS66 family insertion sequence element accessory protein TnpB | - |
| BWI89_RS27940 (BWI89_27925) | 86504..88036 | + | 1533 | WP_000998025 | IS66-like element ISEc8 family transposase | - |
| BWI89_RS27945 (BWI89_27930) | 88070..89347 | - | 1278 | WP_148119647 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BWI89_RS27950 (BWI89_27935) | 89360..89995 | - | 636 | WP_000934979 | A24 family peptidase | - |
| BWI89_RS27955 (BWI89_27940) | 89999..90481 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BWI89_RS27960 (BWI89_27945) | 90547..91104 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BWI89_RS27965 (BWI89_27950) | 91149..92258 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BWI89_RS27970 (BWI89_27955) | 92249..93787 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BWI89_RS27975 (BWI89_27960) | 93812..94306 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BWI89_RS27980 (BWI89_27965) | 94290..95600 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BWI89_RS27985 (BWI89_27970) | 95651..97294 | - | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| BWI89_RS27990 (BWI89_27975) | 97287..97829 | - | 543 | WP_001220544 | sigma 54-interacting transcriptional regulator | virb4 |
| BWI89_RS27995 (BWI89_27980) | 97876..99835 | - | 1960 | Protein_117 | type IV secretory system conjugative DNA transfer family protein | - |
| BWI89_RS28000 (BWI89_27985) | 99851..100907 | - | 1057 | Protein_118 | P-type DNA transfer ATPase VirB11 | - |
| BWI89_RS30280 | 100926..101162 | - | 237 | WP_236919633 | TrbI/VirB10 family protein | - |
| BWI89_RS30285 | 101132..101593 | - | 462 | WP_236919634 | TrbI/VirB10 family protein | virB10 |
| BWI89_RS30290 | 101605..102063 | - | 459 | WP_236919635 | hypothetical protein | - |
| BWI89_RS30380 | 102053..102301 | - | 249 | WP_020246051 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| BWI89_RS30385 | 102298..102615 | - | 318 | WP_335589284 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BWI89_RS28015 (BWI89_28000) | 102883..103557 | - | 675 | WP_236919636 | type IV secretion system protein | virB8 |
| BWI89_RS28025 (BWI89_27380) | 103723..105414 | - | 1692 | Protein_125 | conjugal transfer protein | - |
Host bacterium
| ID | 3377 | GenBank | NZ_CP019277 |
| Plasmid name | p13P477T-4 | Incompatibility group | IncI2 |
| Plasmid size | 105415 bp | Coordinate of oriT [Strand] | 69067..69119 [-]; 6051..6103 [-] |
| Host baterium | Escherichia coli strain 13P477T |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |