Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102931
Name   oriT_p13P484A-4 in_silico
Organism   Escherichia coli strain 13P484A
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP019284 (49816..49916 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_p13P484A-4
TATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2201 GenBank   WP_000884352
Name   TrwC_BWI90_RS27085_p13P484A-4 insolico UniProt ID   A8R732
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120163.41 Da        Isoelectric Point: 6.5766

>WP_000884352.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A8R732


Auxiliary protein

ID   902 GenBank   WP_000706094
Name   WP_000706094_p13P484A-4 insolico UniProt ID   B6UZ32
Length   96 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 96 a.a.        Molecular weight: 10829.54 Da        Isoelectric Point: 8.5040

>WP_000706094.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MKIVADRLSDVNRKLDYLFDRASDADFGPLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIA
EPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB B6UZ32


T4CP

ID   1879 GenBank   WP_071067464
Name   t4cp2_BWI90_RS27090_p13P484A-4 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_071067464.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacteriaceae]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFLGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2860..11789

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI90_RS26755 (BWI90_26720) 119..307 - 189 WP_000957857 hypothetical protein -
BWI90_RS26760 (BWI90_26725) 429..744 - 316 Protein_1 TrbM/KikA/MpfK family conjugal transfer protein -
BWI90_RS26765 (BWI90_26730) 707..1081 - 375 WP_236920024 hypothetical protein -
BWI90_RS28505 1104..1179 - 76 Protein_3 KorB -
BWI90_RS28510 1506..1691 + 186 WP_236920025 hypothetical protein -
BWI90_RS28645 (BWI90_26745) 2231..2512 + 282 Protein_5 transcriptional repressor KorA -
BWI90_RS26790 (BWI90_26755) 2860..3168 + 309 WP_148119859 VirB3 family type IV secretion system protein virB3
BWI90_RS28515 3301..3537 + 237 WP_236920026 hypothetical protein virb4
BWI90_RS28520 3639..3980 + 342 WP_236920027 hypothetical protein -
BWI90_RS28840 4041..4148 + 108 WP_335589268 hypothetical protein -
BWI90_RS28525 4189..4838 + 650 Protein_10 hypothetical protein -
BWI90_RS28530 4924..5217 + 294 WP_236920029 hypothetical protein virb4
BWI90_RS28535 5214..5762 + 549 WP_227543278 hypothetical protein virb4
BWI90_RS28540 5909..6364 + 456 Protein_13 type IV secretion system protein -
BWI90_RS26805 (BWI90_26770) 6500..6727 + 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
BWI90_RS26810 (BWI90_26775) 6743..7783 + 1041 WP_148119860 type IV secretion system protein virB6
BWI90_RS26815 (BWI90_26780) 7866..8012 + 147 WP_001257173 conjugal transfer protein TraN -
BWI90_RS28545 (BWI90_26785) 8002..8220 + 219 WP_148119861 hypothetical protein virB8
BWI90_RS28550 (BWI90_26790) 8259..8624 + 366 WP_250645195 type IV secretion system protein virB8
BWI90_RS28610 8594..8698 + 105 WP_250645194 VirB8/TrbF family protein virB8
BWI90_RS26830 (BWI90_26795) 8709..9593 + 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
BWI90_RS26835 (BWI90_26800) 9593..10752 + 1160 Protein_21 type IV secretion system protein VirB10 -
BWI90_RS26840 (BWI90_26805) 10794..11789 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BWI90_RS26845 (BWI90_26810) 11789..12322 + 534 WP_000731968 phospholipase D family protein -
BWI90_RS28555 (BWI90_26815) 12496..12990 - 495 WP_306415314 DUF6710 family protein -
BWI90_RS26855 (BWI90_26820) 13062..16055 - 2994 WP_104750711 Tn3-like element Tn3 family transposase -
BWI90_RS26860 (BWI90_26825) 16061..16765 - 705 WP_148119862 IS6-like element IS26 family transposase -

Region 2: 69795..80120

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI90_RS27215 (BWI90_27180) 64911..65714 + 804 WP_001082320 aminoglycoside O-phosphotransferase APH(3'')-Ib -
BWI90_RS27220 (BWI90_27185) 65714..66550 + 837 WP_001495812 APH(6)-I family aminoglycoside O-phosphotransferase -
BWI90_RS27225 (BWI90_27195) 66676..66987 - 312 WP_001452736 hypothetical protein -
BWI90_RS27230 (BWI90_27200) 67192..68391 - 1200 WP_000948429 IS91 family transposase -
BWI90_RS27235 (BWI90_27205) 68401..68589 - 189 WP_000957857 hypothetical protein -
BWI90_RS27240 (BWI90_27210) 68711..69025 - 315 WP_000734776 TrbM/KikA/MpfK family conjugal transfer protein -
BWI90_RS27245 (BWI90_27215) 68988..69365 - 378 WP_000504251 hypothetical protein -
BWI90_RS27250 (BWI90_27220) 69381..69686 - 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
BWI90_RS27255 (BWI90_27225) 69795..70529 + 735 WP_000033783 lytic transglycosylase domain-containing protein virB1
BWI90_RS27260 (BWI90_27230) 70538..70819 + 282 WP_000440698 transcriptional repressor KorA -
BWI90_RS27265 (BWI90_27235) 70829..71122 + 294 WP_000209137 TrbC/VirB2 family protein virB2
BWI90_RS27270 (BWI90_27240) 71172..71489 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BWI90_RS27275 (BWI90_27245) 71489..74089 + 2601 WP_001200711 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BWI90_RS27280 (BWI90_27250) 74107..74820 + 714 WP_000749362 type IV secretion system protein virB5
BWI90_RS27285 (BWI90_27255) 74828..75055 + 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
BWI90_RS27290 (BWI90_27260) 75071..76111 + 1041 WP_148119860 type IV secretion system protein virB6
BWI90_RS27295 (BWI90_27265) 76194..76340 + 147 WP_001257173 conjugal transfer protein TraN -
BWI90_RS27300 (BWI90_27270) 76330..77028 + 699 WP_000646594 type IV secretion system protein virB8
BWI90_RS27305 (BWI90_27275) 77039..77923 + 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
BWI90_RS27310 (BWI90_27280) 77923..79083 + 1161 WP_000101711 type IV secretion system protein VirB10 virB10
BWI90_RS27315 (BWI90_27285) 79125..80120 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BWI90_RS27320 (BWI90_27290) 80120..80653 + 534 WP_000731968 phospholipase D family protein -
BWI90_RS28575 (BWI90_27295) 80827..81321 - 495 WP_306415314 DUF6710 family protein -
BWI90_RS27330 (BWI90_27300) 81393..84386 - 2994 WP_104750711 Tn3-like element Tn3 family transposase -
BWI90_RS27335 (BWI90_27305) 84392..85096 - 705 WP_148119862 IS6-like element IS26 family transposase -

Host bacterium

ID   3374 GenBank   NZ_CP019284
Plasmid name   p13P484A-4 Incompatibility group   IncX1
Plasmid size   93578 bp Coordinate of oriT [Strand]   49816..49916 [+]
Host baterium   Escherichia coli strain 13P484A

Cargo genes

Drug resistance gene   catA2, aac(3)-IId, blaTEM-1B, aph(3'')-Ib, aph(6)-Id
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -