Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102928
• Name: oriT_pUO-STmRV1
• Organism: Salmonella enterica subsp. enterica strain LSP 389/97
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP018220 (75038..75188 [-], 151 nt)
• oriT length: 151 nt
• IRs (inverted repeats): 93..98, 106..111 (TGGCCT..AGGCCA) 12..17, 24..29 (AACCCT..AGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 151 nt

>oriT_pUO-STmRV1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

TraI_2_A5895_RS25865_pUO-STmRV1

• ID: 2198
• GenBank: WP_025989290
• Name: TraI_2_A5895_RS25865_pUO-STmRV1
• UniProt ID: A0A345JNN1
• Length: 992 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 992 a.a.
• Molecular weight: 109630.88 Da
• Isoelectric Point: 4.8979

>WP_025989290.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPSKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETASTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
EAFELVSPPGLDASKNKSAPKQQSRKKAQQQKSEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKQVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (51-357)

Protein structure

• AlphaFold DB: A0A345JNN1

T4CP

traD_A5895_RS25860_pUO-STmRV1

• ID: 1874
• GenBank: WP_025989289
• Name: traD_A5895_RS25860_pUO-STmRV1
• UniProt ID: _
• Length: 621 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 621 a.a.
• Molecular weight: 69223.69 Da
• Isoelectric Point: 6.8944

>WP_025989289.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEYPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (471-598)

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (170-297)

Protein structure

No available structure.

Host bacterium

• ID: 3371
• GenBank: NZ_CP018220
• Plasmid name: pUO-STmRV1
• Incompatibility group: IncN
• Plasmid size: 197365 bp
• Coordinate of oriT [Strand]: 75038..75188 [-]
• Host baterium: Salmonella enterica subsp. enterica strain LSP 389/97

Cargo genes

• Drug resistance gene: -
• Virulence gene: spvB, spvC
• Metal resistance gene: silE, silS, silR, silC, silF, silB, silA, silP, merE, merD, merA, merP, merT, merR, arsH
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -