Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102925
Name   oriT_pRACO34Aa in_silico
Organism   Rhizobium sp. ACO-34A
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP021375 (163149..163190 [+], 42 nt)
oriT length   42 nt
IRs (inverted repeats)      22..27, 31..36  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 42 nt

>oriT_pRACO34Aa
GGCTCCAAGGGCGCAATTATACGTCGCGATGCGACGCGTTGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2195 GenBank   WP_099061559
Name   traA_ACO34A_RS29300_pRACO34Aa insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123309.94 Da        Isoelectric Point: 9.5838

>WP_099061559.1 Ti-type conjugative transfer relaxase TraA [Rhizobium sp. ACO-34A]
MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEFEREARTIDYTRKQGLLHEEFMLPPDAPTWVRALIADR
SVSGAVEAFWNKVETFEKRADAQLARDLTIALPVELSAEQNIALVRDFVEQHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLSEDGFGSKKVAIIGEDGQPVRTKSGKILYELWAGSTEDFNVLRDGWFERLNHHLALGG
VDLRVDGRSYEKQGIDLQPTIHLGVRTKAIERKAESQGVRPELERIELNEQRRSENARRVIHNPAIVLDL
ITREKSVFDERDIAKVLYRYVDDPAVFQQLMLKIILSPDVLRLQRDTIDFATGEKLPARYSTRAMIRLEA
TMARHATWLSNREGRGVSHSALDATFKRHERLSDEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIESRTLASWELRWNRGRDVLDEKTIFVMDEAGMVASKQMAGFVD
AVGRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWMRKASLDLARGNVEKALSAYG
ANVRITGERLKADAVERLIADWNHDYDQTKTTLILAHLRRDVRMLNVMAREKLVERGMVGEGHLFRTVDG
ERRFDAGDQIVFLKNEGSLGVKNGMIGNVMEAAANRIVATVGEGDQRRQVVVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGTRSFAYNGGLAAVLSRKNAKETTLDYEQGQLY
REALRFAEARGLHIVQVARTLVRDRLDWTLRQKAKLVDLGQRLAAFAARLGIIQNPDTQTMKEAAPMVAG
IKTISGSVADAVDDKLGADPALKRQWEEVSARFAYVFADAETAFRAMNFDAVLVDKEMAKQVLRKLEMEP
ASIGSLNGKTGILATRSEREARRVAEVNVPALKRELEQYLRMCETVTQRLQMDEQAMRQRVSIDIPALSP
AARVVLERVRDAIDRNDLPAAMAYALSNREAKLEIDGFNQAVTERFGERVLLSNAAREPSGKLYEKLSEG
MKPEQKEQLKKAWPVMRTAQQLAAHERTVQSLKLAEEQRLTQRQTPVLKQ

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

AAA domain (AAA_30)

(382-566)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(690-736)


  Protein structure



No available structure.




T4CP

ID   1871 GenBank   WP_099061503
Name   traG_ACO34A_RS29285_pRACO34Aa insolico UniProt ID   _
Length   650 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 650 a.a.        Molecular weight: 69597.44 Da        Isoelectric Point: 9.7216

>WP_099061503.1 Ti-type conjugative transfer system protein TraG [Rhizobium sp. ACO-34A]
MTVNRLLLATLPAAIMLAAMILSSGMEHRMAALGTSAQAKLMLGRAGLALPYIAAAVIGVIALLATNGST
NIKAAGLSVLAGSAAVITVAIARETIRLNSISSHVPAGQSVLAYVDPATMTGAGIAFIGGVFALRVAMRG
NAAFAASGPRRIGGKRAVHGEAVWMTLPEAAKLFPEPGGIVIGERYSVDRDSVAATPFRADDRQSWGAGG
KSPLLCFDGTFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVSEHRRQAGRKVIVLD
PTAGGVGFNALDWIGRHGNTKEEDIVAVATWIMTDNPRSASARDDFFRASAMQLLTALIADVCLSGHTGE
KDQTLRQVRANLSEPEPKLRARLTKIYEGSESDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYPNYA
GLVSGDSFSTDDLADGGTDIFIALDLKVLEAHPGLARVVIGSLLNAIYNRNGNVKDRTLFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSLGQMREAYGGRDATSKWFESASWISFSAINDPDTADYISKRCGDTT
VEVDQTNRSSGIKGSSRSRSKQLSRRPLILPHEVLRMRSDEQIVFTSGNPPLRCGRAIWFRREDMQTRVA
ANRFHQQSANWPTGNAPARP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-639)

  Protein structure



No available structure.



ID   1872 GenBank   WP_099061535
Name   t4cp2_ACO34A_RS29495_pRACO34Aa insolico UniProt ID   _
Length   820 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 820 a.a.        Molecular weight: 91674.58 Da        Isoelectric Point: 5.9486

>WP_099061535.1 conjugal transfer protein TrbE [Rhizobium sp. ACO-34A]
MVALKSFRHSGPSFADLVPYAGLVDNGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAVRVPTGDYPTEEASHFPDPVTRAIDAERRAHFEKERGHYESRHALVLTWRPPEPRRSGLTR
YVYSDVGSRSATYADKALETFLTSIREVEQYLANVVSIRRMMTRETPERGGHRVARYDELFQFIRFCITG
ENHPVRLPEIPMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFVF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSLDQDAMLMVAETEDAIAEATSQLVAYGYYTPVIVL
FNEDQPSLQEKCEGVRRLIQAEGFGARIETINATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSIWS
GSPVAPCPYYPPASPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYGDAQIFA
FDKGGSMLPLTLGIDGDHYQIGGDAPELGEGRALSFCPLADLSSDADRAWASEWIEMLVALQGVTITPDY
RNAISRQIGLMAESRGRSLSDFVSGVQMREIKHALHHYSVDGPMGQLLDAEEDGLALGAFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFDMGL
GPVALSFVGASGKEDLKRIRALHLEHGAAWPLHWLQQRGIAHAQTLFPIR

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(193-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 197135..208338

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACO34A_RS30655 192185..192321 - 137 Protein_185 fumarylacetoacetate hydrolase family protein -
ACO34A_RS29425 (ACO34A_29345) 192444..193079 + 636 WP_237352026 TetR/AcrR family transcriptional regulator -
ACO34A_RS29430 (ACO34A_29350) 193350..193751 - 402 WP_099061527 tautomerase family protein -
ACO34A_RS29435 (ACO34A_29355) 193978..195081 - 1104 WP_099061528 epoxide hydrolase family protein -
ACO34A_RS29440 (ACO34A_29360) 195129..195962 - 834 WP_099061563 oxidoreductase -
ACO34A_RS29445 (ACO34A_29365) 196206..197120 + 915 WP_099061529 AraC family transcriptional regulator -
ACO34A_RS29450 (ACO34A_29370) 197135..198442 - 1308 WP_099061530 IncP-type conjugal transfer protein TrbI virB10
ACO34A_RS29455 (ACO34A_29375) 198455..198934 - 480 WP_099061531 conjugal transfer protein TrbH -
ACO34A_RS29460 (ACO34A_29380) 198934..199752 - 819 WP_237352027 P-type conjugative transfer protein TrbG virB9
ACO34A_RS29465 (ACO34A_29385) 199803..200465 - 663 WP_099061532 conjugal transfer protein TrbF virB8
ACO34A_RS29470 (ACO34A_29390) 200481..201110 - 630 Protein_195 type IV secretion system protein -
ACO34A_RS29480 (ACO34A_29400) 202624..203196 - 573 Protein_197 P-type conjugative transfer protein TrbL -
ACO34A_RS29485 (ACO34A_29405) 203190..203423 - 234 WP_099061533 entry exclusion protein TrbK -
ACO34A_RS29490 (ACO34A_29410) 203420..204229 - 810 WP_099061534 P-type conjugative transfer protein TrbJ virB5
ACO34A_RS29495 (ACO34A_29415) 204201..206663 - 2463 WP_099061535 conjugal transfer protein TrbE virb4
ACO34A_RS29500 (ACO34A_29420) 206675..206974 - 300 WP_035264674 conjugal transfer protein TrbD virB3
ACO34A_RS29505 (ACO34A_29425) 206967..207377 - 411 WP_099061536 conjugal transfer pilin TrbC virB2
ACO34A_RS29510 (ACO34A_29430) 207367..208338 - 972 WP_099061537 P-type conjugative transfer ATPase TrbB virB11
ACO34A_RS29515 (ACO34A_29435) 208335..208970 - 636 WP_099061538 acyl-homoserine-lactone synthase TraI -
ACO34A_RS29520 (ACO34A_29440) 209346..210548 + 1203 WP_186363650 plasmid partitioning protein RepA -
ACO34A_RS29525 (ACO34A_29445) 210736..211743 + 1008 WP_099061539 plasmid partitioning protein RepB -
ACO34A_RS29530 (ACO34A_29450) 211957..213273 + 1317 WP_099061540 plasmid replication protein RepC -

Host bacterium

ID   3368 GenBank   NZ_CP021375
Plasmid name   pRACO34Aa Incompatibility group   -
Plasmid size   213273 bp Coordinate of oriT [Strand]   163149..163190 [+]
Host baterium   Rhizobium sp. ACO-34A

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   LinJ
Symbiosis gene   nifB, nifZ, nifT, nifA, fixX, fixC, fixB, fixA, nifW, nifH, nifD, nifK, nifE, nifX, mLTONO_5203
Anti-CRISPR   -