Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102922 |
| Name | oriT_p1540-1  |
| Organism | Escherichia coli strain CRE1540 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP019052 (18937..18989 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p1540-1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1869 | GenBank | WP_015059539 |
| Name | t4cp2_BVL39_RS25820_p1540-1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 37344..60801
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BVL39_RS25755 (BVL39_26085) | 32674..33327 | - | 654 | WP_170852113 | hypothetical protein | - |
| BVL39_RS25760 (BVL39_26090) | 33339..34463 | - | 1125 | WP_000486716 | site-specific integrase | - |
| BVL39_RS29035 | 35091..35426 | + | 336 | WP_158638052 | hypothetical protein | - |
| BVL39_RS25770 (BVL39_26100) | 35436..36692 | - | 1257 | WP_236918904 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BVL39_RS25775 (BVL39_26105) | 36705..37340 | - | 636 | WP_000934977 | A24 family peptidase | - |
| BVL39_RS25780 (BVL39_26110) | 37344..37826 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| BVL39_RS25785 (BVL39_26115) | 37892..38449 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BVL39_RS25790 (BVL39_26120) | 38494..39603 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BVL39_RS25795 (BVL39_26125) | 39594..41132 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BVL39_RS25800 (BVL39_26130) | 41157..41651 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BVL39_RS25805 (BVL39_26135) | 41635..42945 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BVL39_RS25810 (BVL39_26140) | 42996..44639 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| BVL39_RS25815 (BVL39_26145) | 44632..45168 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| BVL39_RS25820 (BVL39_26150) | 45215..47173 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| BVL39_RS25825 (BVL39_26155) | 47189..48244 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BVL39_RS25830 (BVL39_26160) | 48263..49402 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| BVL39_RS25835 (BVL39_26165) | 49392..50093 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BVL39_RS25840 (BVL39_26170) | 50159..50893 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| BVL39_RS25850 (BVL39_26180) | 51059..53416 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| BVL39_RS25855 (BVL39_26185) | 53422..53742 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BVL39_RS29720 (BVL39_26190) | 53813..54103 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| BVL39_RS25865 (BVL39_26195) | 54103..54687 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| BVL39_RS25870 (BVL39_26200) | 54708..55106 | - | 399 | WP_072643816 | hypothetical protein | - |
| BVL39_RS25875 (BVL39_26205) | 55225..55662 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BVL39_RS25880 (BVL39_26210) | 55668..56903 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| BVL39_RS25885 (BVL39_26215) | 56906..57205 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BVL39_RS25890 (BVL39_26220) | 57273..57554 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| BVL39_RS25895 (BVL39_26225) | 57544..57795 | - | 252 | WP_000121741 | hypothetical protein | - |
| BVL39_RS25900 (BVL39_26230) | 57895..58530 | - | 636 | WP_015059536 | hypothetical protein | - |
| BVL39_RS25905 (BVL39_26235) | 58603..58890 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| BVL39_RS25910 (BVL39_26240) | 58903..59157 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| BVL39_RS25915 (BVL39_26245) | 59159..59800 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| BVL39_RS25920 (BVL39_26250) | 59806..60801 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| BVL39_RS25925 (BVL39_26255) | 60805..61062 | - | 258 | WP_000739144 | hypothetical protein | - |
| BVL39_RS25930 (BVL39_26260) | 61059..61361 | - | 303 | WP_001360345 | hypothetical protein | - |
| BVL39_RS25935 (BVL39_26265) | 61342..61599 | - | 258 | WP_001542015 | hypothetical protein | - |
| BVL39_RS25940 (BVL39_26270) | 61632..62078 | - | 447 | WP_001243165 | hypothetical protein | - |
| BVL39_RS29290 | 62089..62259 | - | 171 | WP_000550720 | hypothetical protein | - |
| BVL39_RS25945 (BVL39_26275) | 62263..62706 | - | 444 | WP_000964330 | NfeD family protein | - |
| BVL39_RS25955 (BVL39_26285) | 63080..64033 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BVL39_RS29295 | 64060..64236 | - | 177 | WP_000753050 | hypothetical protein | - |
| BVL39_RS25960 (BVL39_26290) | 64229..64444 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| BVL39_RS25965 (BVL39_26295) | 64437..64889 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3365 | GenBank | NZ_CP019052 |
| Plasmid name | p1540-1 | Incompatibility group | IncI2 |
| Plasmid size | 65533 bp | Coordinate of oriT [Strand] | 18937..18989 [-] |
| Host baterium | Escherichia coli strain CRE1540 |
Cargo genes
| Drug resistance gene | blaCTX-M-55, mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |