Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102918
• Name: oriT_pSymB
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP019586 (695803..695831 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSymB
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_SMB554_RS28980_pSymB

• ID: 2190
• GenBank: WP_088896481
• Name: traA_SMB554_RS28980_pSymB
• UniProt ID: _
• Length: 1539 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1539 a.a.
• Molecular weight: 169654.39 Da
• Isoelectric Point: 9.6129

>WP_088896481.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium meliloti]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRTRMIDEQAGTSFSYRGGASELVHEELALPDDIPAWLKAAIDG
RSVAKASEALWNAVEAHETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTEQGFGPKKVPVLGEDGEPLRVVTPDRPNGKIVYKLWAGDKETIKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGVAMYFAPADLARRQEMADRLLAEPELLLKQ
LGNERSTFDERDIARALHRYVDDPVDFANIRARLMASDDLVLLKPQQIDAETGKAKQPAVFTTREMLRLE
YAMAQSAEVLSRRKGFGVSNARAAAAARSIETADTEKPFRLDPEQVDAVRHVTRDNAIAAVVGLAGAGKS
TLLAAARVAWEGEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGREQLQRGDVLVIDEAGMVS
SQQMARVLKAVEDAGAKAVLVGDAMQLQPIEAGAAFRAITERIGFAELAGVRRQRDAWARDASRLFARGK
VEEGLDAYAQQGRIVETETRAEIVDRIVSDWANARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLN
TSLRNVMIGEGALAGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTVVSTGDRR
GDTLLSVRLDSGRDVVISEDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADL
YAAKEDFEAKPEWGRKPRVDHAAGVTGELVKEGMAKFRPNDEDADESPYADIRTDDGTVQRLWGVSLPKA
LKDAGAAEGDTITLRKDGVERVKVQVPIVDAQTGEKRFEERQVDRNVWSASQLETAAARRERIERESHRP
QLFAVLVERLSRSGAKTTTLDFEGEAGYQAQARDFARRRGLYHLSLVAAGMEAEVLRRWAGIAEKREQVA
KLWERASVALGFAIERERRVSYNEERTETLSTGIPSDGKYLVPPTTTFSRSVAEDARLAQLSSQRWKERE
AIVHPVLAKIYRDPDGALSALNALASDAAIEPRKLAEDLGLAPDRLGRLRGSELVVDGRAARDERTAATV
ALSELLPLARAHATEFRRNAERFGIREQQRRAHMALSVPALAKTAMARLVEIEAVRKAGGEDAYRAALTF
AVEDRLLVQEVKAVNEALTARFGWSAFTAKADVIAERNIAERMPEDLAPERREKLTRLFAVIRRFAEEQH
LAERQDRSKIVAGASVELGKGTFAVLPMLAAVTEFKTTVDEEARERALAAPHYAHHRAALVETATRVWRD
PADAIGKIEDLIVKGFAAERIAAAVTNDPAAYGALRGSDRIMDKLLAVGRERKGALQAVPEAASRVRSLG
ASYASALDAETRSITEERRRMAVTIPGLSPAAEDALKRLAAQIKNKDGKLDVAAGSLDPHIAREFAKVSR
ALDERFGRNAILRGETDVINRVSPAQRRAFEAMRDRLTILQQAVRIQSSQKIVSERRQRAINQSRGIRM

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1125)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1309-1400)

• AAA domain (AAA_30) (391-578)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

T4CP

tcpA_SMB554_RS33505_pSymB

• ID: 1863
• GenBank: WP_089413699
• Name: tcpA_SMB554_RS33505_pSymB
• UniProt ID: _
• Length: 946 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 946 a.a.
• Molecular weight: 101690.47 Da
• Isoelectric Point: 4.6104

>WP_089413699.1 FtsK/SpoIIIE domain-containing protein [Sinorhizobium meliloti]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
AARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEES
RIEADEAAAEASDAETVMDVVPAEPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQAPEASVTKPAI
TESVAVAEEGPATSETTALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQADARLAYLSDFAFFEFMPL
EVAAVPPTVTEPVKEAARIPAPIAAAPKVSPRKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEPV
TAAPQDIAAEPAAAEAAALPAHQFVETPAPALAEPAIVPASEPAPEAPVTRAAITMPAVIQRSSPSLPPI
GAIEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEF
EPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLAL
CLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIP
HLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTGE
PLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKA
NFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRPE
YLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVE
RMEKDGLVGPANHVGKREIIYGNRDNPPKPESDDLD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (543-761)

• Ftsk gamma domain (FtsK_gamma) (871-930)

• FtsK alpha domain (FtsK_alpha) (434-534)

Protein structure

No available structure.

Host bacterium

• ID: 3361
• GenBank: NZ_CP019586
• Plasmid name: pSymB
• Incompatibility group: -
• Plasmid size: 1639840 bp
• Coordinate of oriT [Strand]: 695803..695831 [+]
• Host baterium: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Cargo genes

• Drug resistance gene: -
• Virulence gene: htpB
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA7