Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102902
Name   oriT_A_020709_82|unnamed2 in_silico
Organism   Enterococcus faecium strain A_020709_82
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP018130 (51436..51627 [-], 192 nt)
oriT length   192 nt
IRs (inverted repeats)      106..112, 122..128  (ATTTTTT..AAAAAAT)
 107..113, 120..126  (TTTTTTG..CAAAAAA)
 93..98, 105..110  (AAAATA..TATTTT)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..49, 52..60  (CACCTTCCT..AGGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 192 nt

>oriT_A_020709_82|unnamed2
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2180 GenBank   WP_002326870
Name   MobL_BSG28_RS15480_A_020709_82|unnamed2 insolico UniProt ID   B5U8V6
Length   506 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 506 a.a.        Molecular weight: 60597.01 Da        Isoelectric Point: 9.3485

>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(11-438)


  Protein structure


Source ID Structure
AlphaFold DB B5U8V6


T4CP

ID   1853 GenBank   WP_230458036
Name   t4cp2_BSG28_RS15405_A_020709_82|unnamed2 insolico UniProt ID   _
Length   605 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 605 a.a.        Molecular weight: 68148.50 Da        Isoelectric Point: 9.2509

>WP_230458036.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNSVTCLA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(199-443)

  Protein structure



No available structure.




Host bacterium

ID   3345 GenBank   NZ_CP018130
Plasmid name   A_020709_82|unnamed2 Incompatibility group   -
Plasmid size   71104 bp Coordinate of oriT [Strand]   51436..51627 [-]
Host baterium   Enterococcus faecium strain A_020709_82

Cargo genes

Drug resistance gene   VanHAX
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -