Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102900 |
| Name | oriT_pSE69-3861-1  |
| Organism | Salmonella enterica subsp. enterica serovar Enteritidis strain 69-3861 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018638 (34251..34339 [-], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_pSE69-3861-1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2178 | GenBank | WP_001545749 |
| Name | nikB_BTN68_RS24280_pSE69-3861-1 |
UniProt ID | A7ZGZ9 |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 103891.35 Da Isoelectric Point: 7.3473
>WP_001545749.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A7ZGZ9 |
Auxiliary protein
| ID | 887 | GenBank | WP_001283947 |
| Name | WP_001283947_pSE69-3861-1 |
UniProt ID | A0A142CMC2 |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12613.57 Da Isoelectric Point: 10.7463
>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMC2 |
T4CP
| ID | 1850 | GenBank | WP_001545750 |
| Name | trbC_BTN68_RS24285_pSE69-3861-1 |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86890.12 Da Isoelectric Point: 6.7712
>WP_001545750.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVIMESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTAKTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGISLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVIMESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTAKTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGISLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 39717..79208
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BTN68_RS24285 (BTN68_24185) | 37433..39724 | - | 2292 | WP_001545750 | F-type conjugative transfer protein TrbC | - |
| BTN68_RS24290 (BTN68_24190) | 39717..40787 | - | 1071 | WP_064767856 | IncI1-type conjugal transfer protein TrbB | trbB |
| BTN68_RS24295 (BTN68_24195) | 40806..42014 | - | 1209 | WP_000121263 | IncI1-type conjugal transfer protein TrbA | trbA |
| BTN68_RS24300 (BTN68_24200) | 42321..43100 | - | 780 | WP_275450201 | protein FinQ | - |
| BTN68_RS24305 (BTN68_24205) | 43727..43879 | + | 153 | WP_001387489 | Hok/Gef family protein | - |
| BTN68_RS24310 (BTN68_24210) | 43951..44202 | - | 252 | WP_001291964 | hypothetical protein | - |
| BTN68_RS25790 | 44703..44798 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| BTN68_RS25370 | 44863..45039 | - | 177 | WP_001054898 | hypothetical protein | - |
| BTN68_RS24320 (BTN68_24220) | 45222..45431 | - | 210 | WP_001140543 | hemolysin expression modulator Hha | - |
| BTN68_RS24325 (BTN68_24225) | 45529..46143 | - | 615 | WP_000578648 | plasmid IncI1-type surface exclusion protein ExcA | - |
| BTN68_RS24330 (BTN68_24230) | 46219..48387 | - | 2169 | WP_000698360 | IncI1-type conjugal transfer membrane protein TraY | traY |
| BTN68_RS24335 (BTN68_24235) | 48484..49068 | - | 585 | WP_001037983 | IncI1-type conjugal transfer protein TraX | - |
| BTN68_RS24340 (BTN68_24240) | 49097..50299 | - | 1203 | WP_001189160 | IncI1-type conjugal transfer protein TraW | traW |
| BTN68_RS24345 (BTN68_24245) | 50266..50880 | - | 615 | WP_000337398 | IncI1-type conjugal transfer protein TraV | traV |
| BTN68_RS24350 (BTN68_24250) | 50880..53924 | - | 3045 | WP_099917990 | IncI1-type conjugal transfer protein TraU | traU |
| BTN68_RS24355 (BTN68_24255) | 54014..54814 | - | 801 | WP_001164783 | IncI1-type conjugal transfer protein TraT | traT |
| BTN68_RS24360 (BTN68_24260) | 54798..54986 | - | 189 | WP_001277253 | putative conjugal transfer protein TraS | - |
| BTN68_RS24365 (BTN68_24265) | 55050..55454 | - | 405 | WP_000086965 | IncI1-type conjugal transfer protein TraR | traR |
| BTN68_RS24370 (BTN68_24270) | 55505..56032 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| BTN68_RS24375 (BTN68_24275) | 56032..56736 | - | 705 | WP_000801918 | IncI1-type conjugal transfer protein TraP | traP |
| BTN68_RS24380 (BTN68_24280) | 56736..58025 | - | 1290 | WP_001271994 | conjugal transfer protein TraO | traO |
| BTN68_RS24385 (BTN68_24285) | 58028..59011 | - | 984 | WP_001191879 | IncI1-type conjugal transfer protein TraN | traN |
| BTN68_RS24390 (BTN68_24290) | 59022..59714 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| BTN68_RS24395 (BTN68_24295) | 59711..60058 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| BTN68_RS24400 (BTN68_24300) | 60076..63840 | - | 3765 | WP_099917991 | LPD7 domain-containing protein | - |
| BTN68_RS24405 (BTN68_24305) | 63930..64481 | - | 552 | WP_000014586 | phospholipase D family protein | - |
| BTN68_RS24410 (BTN68_24310) | 64496..64786 | - | 291 | WP_001314267 | hypothetical protein | traK |
| BTN68_RS24415 (BTN68_24315) | 64783..65931 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| BTN68_RS24420 (BTN68_24320) | 65928..66746 | - | 819 | WP_052962236 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| BTN68_RS24425 (BTN68_24325) | 66743..67201 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| BTN68_RS24430 (BTN68_24330) | 67596..68180 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| BTN68_RS24435 (BTN68_24335) | 68240..69442 | - | 1203 | WP_064767858 | conjugal transfer protein TraF | - |
| BTN68_RS24440 (BTN68_24340) | 69528..70352 | - | 825 | WP_001238932 | conjugal transfer protein TraE | traE |
| BTN68_RS24445 (BTN68_24345) | 70503..71657 | - | 1155 | WP_001139958 | site-specific integrase | - |
| BTN68_RS25690 | 71656..72009 | + | 354 | WP_001393368 | hypothetical protein | - |
| BTN68_RS25855 (BTN68_24380) | 72890..73114 | - | 225 | Protein_85 | hypothetical protein | - |
| BTN68_RS24495 (BTN68_24390) | 73387..74715 | - | 1329 | WP_069057437 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BTN68_RS24500 (BTN68_24395) | 74715..75371 | - | 657 | WP_001193549 | A24 family peptidase | - |
| BTN68_RS24505 (BTN68_24400) | 75356..75916 | - | 561 | WP_000014005 | lytic transglycosylase domain-containing protein | virB1 |
| BTN68_RS24510 (BTN68_24405) | 75926..76540 | - | 615 | WP_000908227 | type 4 pilus major pilin | - |
| BTN68_RS24515 (BTN68_24410) | 76557..77642 | - | 1086 | WP_001208802 | type II secretion system F family protein | - |
| BTN68_RS24520 (BTN68_24415) | 77655..79208 | - | 1554 | WP_001545756 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BTN68_RS24525 (BTN68_24420) | 79219..79671 | - | 453 | WP_064767859 | type IV pilus biogenesis protein PilP | - |
| BTN68_RS24530 (BTN68_24425) | 79658..80953 | - | 1296 | WP_001545757 | type 4b pilus protein PilO2 | - |
| BTN68_RS24535 (BTN68_24430) | 80946..82628 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| BTN68_RS24540 (BTN68_24435) | 82642..83079 | - | 438 | WP_001545758 | type IV pilus biogenesis protein PilM | - |
| BTN68_RS24545 (BTN68_24440) | 83079..84146 | - | 1068 | WP_016245573 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 3343 | GenBank | NZ_CP018638 |
| Plasmid name | pSE69-3861-1 | Incompatibility group | IncI1 |
| Plasmid size | 95773 bp | Coordinate of oriT [Strand] | 34251..34339 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Enteritidis strain 69-3861 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |