Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102899
Name   oriT_pSE79-2359 in_silico
Organism   Salmonella enterica subsp. enterica serovar Enteritidis strain 79-2359
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP018646 (49814..49914 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pSE79-2359
TATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2177 GenBank   WP_000884352
Name   TrwC_BTN70_RS23895_pSE79-2359 insolico UniProt ID   A8R732
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120163.41 Da        Isoelectric Point: 6.5766

>WP_000884352.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A8R732


Auxiliary protein

ID   886 GenBank   WP_000706094
Name   WP_000706094_pSE79-2359 insolico UniProt ID   B6UZ32
Length   96 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 96 a.a.        Molecular weight: 10829.54 Da        Isoelectric Point: 8.5040

>WP_000706094.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MKIVADRLSDVNRKLDYLFDRASDADFGPLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIA
EPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB B6UZ32


T4CP

ID   1849 GenBank   WP_000342688
Name   t4cp2_BTN70_RS24185_pSE79-2359 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12383..22708

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTN70_RS23925 (BTN70_23870) 7640..8200 + 561 WP_001161490 recombinase family protein -
BTN70_RS23930 (BTN70_23875) 8204..11170 + 2967 WP_001138014 Tn3-like element TnAs1 family transposase -
BTN70_RS24830 11281..11676 + 396 WP_306766596 DUF6710 family protein -
BTN70_RS23940 (BTN70_23885) 11850..12383 - 534 WP_000731968 phospholipase D family protein -
BTN70_RS23945 (BTN70_23890) 12383..13378 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BTN70_RS23950 (BTN70_23895) 13420..14580 - 1161 WP_000101711 type IV secretion system protein VirB10 virB10
BTN70_RS23955 (BTN70_23900) 14580..15464 - 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
BTN70_RS23960 (BTN70_23905) 15475..16173 - 699 WP_000646594 type IV secretion system protein virB8
BTN70_RS24835 (BTN70_23910) 16163..16321 - 159 WP_012561180 hypothetical protein -
BTN70_RS23970 (BTN70_23915) 16392..17432 - 1041 WP_000886022 type IV secretion system protein virB6
BTN70_RS23975 (BTN70_23920) 17448..17675 - 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
BTN70_RS23980 (BTN70_23925) 17683..18396 - 714 WP_000749362 type IV secretion system protein virB5
BTN70_RS23985 (BTN70_23930) 18414..21014 - 2601 WP_001200711 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BTN70_RS23990 (BTN70_23935) 21014..21331 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BTN70_RS23995 (BTN70_23940) 21381..21674 - 294 WP_000209137 TrbC/VirB2 family protein virB2
BTN70_RS24000 (BTN70_23945) 21684..21965 - 282 WP_000440698 transcriptional repressor KorA -
BTN70_RS24005 (BTN70_23950) 21974..22708 - 735 WP_000033783 lytic transglycosylase domain-containing protein virB1
BTN70_RS24010 (BTN70_23955) 22817..23122 + 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
BTN70_RS24710 23138..23314 + 177 WP_232516986 hypothetical protein -
BTN70_RS24020 (BTN70_23965) 23477..23791 + 315 WP_000734776 TrbM/KikA/MpfK family conjugal transfer protein -
BTN70_RS24025 (BTN70_23970) 23827..24138 + 312 WP_001452736 hypothetical protein -
BTN70_RS24030 (BTN70_23980) 24264..25100 - 837 WP_000480968 aminoglycoside O-phosphotransferase APH(6)-Id -
BTN70_RS24035 (BTN70_23985) 25100..25903 - 804 WP_001082319 aminoglycoside O-phosphotransferase APH(3'')-Ib -

Host bacterium

ID   3342 GenBank   NZ_CP018646
Plasmid name   pSE79-2359 Incompatibility group   IncN
Plasmid size   54796 bp Coordinate of oriT [Strand]   49814..49914 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Enteritidis strain 79-2359

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -