Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102895
Name   oriT_pSE81-1706 in_silico
Organism   Salmonella enterica subsp. enterica serovar Enteritidis strain 81-1706
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP018656 (192224..192508 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pSE81-1706
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2173 GenBank   WP_099828421
Name   TraI_2_BTN76_RS25255_pSE81-1706 insolico UniProt ID   _
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 113051.06 Da        Isoelectric Point: 4.5108

>WP_099828421.1 MobH family relaxase [Salmonella enterica]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAVIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure



No available structure.




T4CP

ID   1844 GenBank   WP_000167418
Name   traD_BTN76_RS25260_pSE81-1706 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 8920..18088

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTN76_RS24105 (BTN76_23970) 4369..4569 - 201 WP_000357614 hypothetical protein -
BTN76_RS24110 (BTN76_23975) 4587..5663 - 1077 WP_001015069 hypothetical protein -
BTN76_RS24115 (BTN76_23980) 6198..7073 + 876 WP_001282731 RepB family plasmid replication initiator protein -
BTN76_RS25730 7520..7864 - 345 WP_153253765 hypothetical protein -
BTN76_RS24125 (BTN76_23990) 8515..8868 + 354 WP_000424121 hypothetical protein -
BTN76_RS24130 (BTN76_23995) 8920..9237 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
BTN76_RS24135 (BTN76_24000) 9249..10034 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
BTN76_RS24140 (BTN76_24005) 10037..11269 + 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
BTN76_RS24145 (BTN76_24010) 11271..11711 + 441 WP_001224093 plasmid transfer protein HtdO -
BTN76_RS24150 (BTN76_24015) 11701..13059 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
BTN76_RS24155 (BTN76_24020) 13068..13580 + 513 WP_000429933 hypothetical protein -
BTN76_RS24160 (BTN76_24025) 13686..14438 + 753 WP_010892304 protein-disulfide isomerase HtdT -
BTN76_RS24165 (BTN76_24030) 14448..15398 + 951 WP_001022266 TraV family lipoprotein traV
BTN76_RS24170 (BTN76_24035) 15407..18088 + 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
BTN76_RS24180 (BTN76_24050) 19132..21129 + 1998 WP_001541890 choline BCCT transporter BetT -
BTN76_RS24185 (BTN76_24055) 21192..22487 + 1296 WP_000625666 DUF2254 domain-containing protein -

Host bacterium

ID   3338 GenBank   NZ_CP018656
Plasmid name   pSE81-1706 Incompatibility group   IncHI1A
Plasmid size   244210 bp Coordinate of oriT [Strand]   192224..192508 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Enteritidis strain 81-1706

Cargo genes

Drug resistance gene   catA1, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, tet(B)
Virulence gene   spvB, spvC, pefB, pefA, pefD, rck
Metal resistance gene   corA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -