Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102885 |
| Name | oriT_p3-T1  |
| Organism | Acidovorax sp. T1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP021651 (23508..23618 [+], 111 nt) |
| oriT length | 111 nt |
| IRs (inverted repeats) | 71..78, 81..88 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 111 nt
>oriT_p3-T1
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2165 | GenBank | WP_061070721 |
| Name | Relaxase_CCX87_RS20430_p3-T1 |
UniProt ID | A0A0X8NUF3 |
| Length | 609 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 609 a.a. Molecular weight: 66772.53 Da Isoelectric Point: 10.5825
>WP_061070721.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICDGLGLGEHQRVSAVHNDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCTTLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKDAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQAAPARRNYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRRKDRAIEHAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQATAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHA
PVIDSITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRPGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPVQAP
TQATPTPPPAKVQQPARPAPSELEQRETRLRAQMEAKKAKRQEGKKRGR
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICDGLGLGEHQRVSAVHNDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCTTLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKDAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQAAPARRNYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRRKDRAIEHAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQATAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHA
PVIDSITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRPGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPVQAP
TQATPTPPPAKVQQPARPAPSELEQRETRLRAQMEAKKAKRQEGKKRGR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0X8NUF3 |
Auxiliary protein
| ID | 873 | GenBank | WP_054471493 |
| Name | WP_054471493_p3-T1 |
UniProt ID | A0A120LKZ5 |
| Length | 125 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 125 a.a. Molecular weight: 13520.32 Da Isoelectric Point: 7.9653
>WP_054471493.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Pseudomonadota]
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGQS
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGQS
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A120LKZ5 |
Host bacterium
| ID | 3328 | GenBank | NZ_CP021651 |
| Plasmid name | p3-T1 | Incompatibility group | - |
| Plasmid size | 56357 bp | Coordinate of oriT [Strand] | 23508..23618 [+] |
| Host baterium | Acidovorax sp. T1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | merR2, merT, merP, merF, merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |