Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102884
Name   oriT_pFORC44_1 in_silico
Organism   Escherichia coli strain FORC_044
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP018625 (71310..71396 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pFORC44_1
GGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2163 GenBank   WP_001354015
Name   nikB_FORC44_RS29910_pFORC44_1 insolico UniProt ID   E8XLT1
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104018.47 Da        Isoelectric Point: 7.3526

>WP_001354015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB E8XLT1


Auxiliary protein

ID   872 GenBank   WP_001281051
Name   WP_001281051_pFORC44_1 insolico UniProt ID   A0A3U8I0C6
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12646.44 Da        Isoelectric Point: 10.4030

>WP_001281051.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A3U8I0C6


T4CP

ID   1833 GenBank   WP_001289276
Name   trbC_FORC44_RS29915_pFORC44_1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 76775..111671

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC44_RS29915 (FORC44_p112) 74491..76782 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
FORC44_RS29920 (FORC44_p113) 76775..77845 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
FORC44_RS29925 (FORC44_p114) 77864..79072 - 1209 WP_000121274 IncI1-type conjugal transfer protein TrbA trbA
FORC44_RS29930 (FORC44_p115) 79364..79516 + 153 WP_001331364 Hok/Gef family protein -
FORC44_RS31695 (FORC44_p116) 79588..79779 - 192 WP_174715448 hypothetical protein -
FORC44_RS32545 80340..80435 + 96 WP_000609148 DinQ-like type I toxin DqlB -
FORC44_RS31700 (FORC44_p118) 80500..80676 - 177 WP_001054904 hypothetical protein -
FORC44_RS29940 (FORC44_p120) 81068..81277 + 210 WP_000062603 HEAT repeat domain-containing protein -
FORC44_RS29945 (FORC44_p121) 81349..82011 - 663 WP_000653334 plasmid IncI1-type surface exclusion protein ExcA -
FORC44_RS29950 (FORC44_p122) 82076..84238 - 2163 WP_000698351 DotA/TraY family protein traY
FORC44_RS29955 (FORC44_p123) 84335..84919 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
FORC44_RS29960 (FORC44_p124) 84948..86150 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
FORC44_RS29965 (FORC44_p125) 86117..86731 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
FORC44_RS29970 (FORC44_p126) 86731..89775 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
FORC44_RS29975 (FORC44_p127) 89865..90665 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
FORC44_RS29980 (FORC44_p128) 90649..90837 - 189 WP_001277255 putative conjugal transfer protein TraS -
FORC44_RS29985 (FORC44_p129) 90901..91305 - 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
FORC44_RS29990 (FORC44_p130) 91356..91883 - 528 WP_001055569 conjugal transfer protein TraQ traQ
FORC44_RS29995 (FORC44_p131) 91883..92587 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
FORC44_RS30000 (FORC44_p132) 92587..93876 - 1290 WP_001272005 conjugal transfer protein TraO traO
FORC44_RS30005 (FORC44_p133) 93879..94862 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
FORC44_RS30010 (FORC44_p134) 94873..95565 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
FORC44_RS30015 (FORC44_p135) 95562..95909 - 348 WP_001055900 conjugal transfer protein traL
FORC44_RS30020 (FORC44_p136) 95927..99694 - 3768 WP_001141529 LPD7 domain-containing protein -
FORC44_RS30025 (FORC44_p137) 99784..100335 - 552 WP_000014583 phospholipase D family protein -
FORC44_RS30030 (FORC44_p138) 100350..100640 - 291 WP_001299214 hypothetical protein traK
FORC44_RS30035 (FORC44_p139) 100637..101785 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
FORC44_RS30040 (FORC44_p140) 101782..102600 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
FORC44_RS30045 (FORC44_p141) 102597..103055 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
FORC44_RS30050 (FORC44_p142) 103450..104034 - 585 WP_000977522 histidine phosphatase family protein -
FORC44_RS30055 (FORC44_p143) 104094..105296 - 1203 WP_000976351 conjugal transfer protein TraF -
FORC44_RS30060 (FORC44_p144) 105381..106205 - 825 WP_001238939 conjugal transfer protein TraE traE
FORC44_RS30065 (FORC44_p145) 106356..107510 - 1155 WP_001139957 site-specific integrase -
FORC44_RS30095 (FORC44_p148) 109373..110461 - 1089 Protein_126 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
FORC44_RS30100 (FORC44_p149) 110461..111117 - 657 WP_001193553 prepilin peptidase -
FORC44_RS30105 (FORC44_p150) 111102..111671 - 570 WP_236918765 lytic transglycosylase domain-containing protein virB1

Host bacterium

ID   3327 GenBank   NZ_CP018625
Plasmid name   pFORC44_1 Incompatibility group   IncI1
Plasmid size   111755 bp Coordinate of oriT [Strand]   71310..71396 [-]
Host baterium   Escherichia coli strain FORC_044

Cargo genes

Drug resistance gene   aph(3')-Ia, aph(6)-Id, aph(3'')-Ib, sul2, blaTEM-1B
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -