Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102881
Name   oriT_MN05|unnamed5 in_silico
Organism   Enterococcus wangshanyuanii strain MN05
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP021878 (43193..43242 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      34..35
Conserved sequence flanking the
  nic site    		  GCTTGCAAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_MN05|unnamed5
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGTCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2160 GenBank   WP_088272015
Name   Relaxase_CC204_RS20865_MN05|unnamed5 insolico UniProt ID   _
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65239.42 Da        Isoelectric Point: 6.0784

>WP_088272015.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus wangshanyuanii]
MVYTKHFVIHTFDKLNNACGYIENAEKTIVTNEDTQDHLAHLFQYIANDEKTNMKQLVSGHGIIDPTNAY
EEFKFTKLRTAFQQKKGYTLDLKTNKLVPPSLTELERKNTVLAHHLIQSFSPDDNLSPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHIHNHILFNSTNLKTGKAFRWQKGTKRVYEQISDKIAAKEGAKIIEKSPRQT
HTKYTKWQTESLFKQKIKSRIDFLLEHSSSIDDFLHKASALNLSVDFSKKWSTYKLLDEPQLRNTRGRSL
SKKNPDRYNYEAIVERLKENTTIFSIEDVVTRYEEVETKNENDFDYQLTIEPWQISHKTERGYYLNIDYG
FGNHGKLFIGGYKVDPLEDGNFNIYVKRNDYFYFMNDKSSSDSKYLTGTSLIRQLRLYNGTTPLKKEPVM
RTIDQLVDAINFLAEHKIEGTRQLNTLEEKLIAAFSEAEETLDHLDEKIIELNNLGKLLLEKETQGDTEE
IQKQLHEILTDATLSEFTFEDVQGEIESITLSQSLLESKLENTRNEIDKLHEIQAIQSQKEEQEKNLKPK
I

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(36-296)


  Protein structure



No available structure.




Auxiliary protein

ID   869 GenBank   WP_088272017
Name   WP_088272017_MN05|unnamed5 insolico UniProt ID   _
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13965.12 Da        Isoelectric Point: 10.2867

>WP_088272017.1 plasmid mobilization relaxosome protein MobC [Enterococcus wangshanyuanii]
MENKNKLRRPIQRIIRFSKEENRLINKKVEESFFPNFQNFALHMLIQGEVRHIDYSELKQLTMEIHKIGI
NINQVTRLANQFNEISSTDIQQLKNELTALTETVQSNLATLIKLKERT

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(63-105)


  Protein structure



No available structure.




T4CP

ID   1830 GenBank   WP_088272023
Name   t4cp2_CC204_RS20890_MN05|unnamed5 insolico UniProt ID   _
Length   603 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 603 a.a.        Molecular weight: 69991.81 Da        Isoelectric Point: 5.6131

>WP_088272023.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus wangshanyuanii]
MQIYKKKLKPYLILACVLFVLGFLIGNFFWLLPGYDYSSKLNYLFTRELLPYVESSFFSFFITPNFLAFG
VGFLSFFSGLLMYVYDNDRGVYRNGEEYGSARYATKEEMKKYEDEIPQNNKILSKNAKVSLFNSRLDIKL
QKNKNDLVIGDSGAAKTLSYIKTNIMQRNCSFLLTDPDGGVVHEVGHLLKEDNYKIKILDLNTLSNSDKF
NVFKYMKTELDVDRVLEAVTEATKKGDQEDIDFWPKAEGLLIRSFIAYLWFDGQDNDYLPHLGMIADMLR
FVKRQDPKVPSPVEEWFEEQNERHPNNYAYKQWSLFNELFEAETRASVLGIAAARYSVFDHEQVVDLIRE
DTMDIDSWTEEKTAIFIAIPETNDAYNFISALFMATVAEQLRVKSDAVRLGKLKLEKGKKLLHFRFLMDE
FANIGKIPHFEKLLTTFRKREMSFAIILQSLNQLQAMYKNSWENIVNGCASLIFLGGDEEKTAKYLSQRI
GKQTLSIRKHSINKSRNGGASENRDKYGRDLLDTSEVTKIDGDECLVFITKEHVFRDKKFYAFDHELADE
LGTNPDDPKWFTYKRYRNEEEELLDKVRKEDIVDHGTIMDDAA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-556)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2954..9729

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CC204_RS20580 348..602 - 255 WP_088271964 hypothetical protein -
CC204_RS20585 826..1098 - 273 WP_088271965 hypothetical protein -
CC204_RS20590 1146..1736 - 591 WP_088271966 hypothetical protein -
CC204_RS20595 1872..2072 - 201 WP_088271967 hypothetical protein -
CC204_RS21305 2534..2686 - 153 WP_157894350 hypothetical protein -
CC204_RS21645 2954..3349 - 396 WP_227011296 hypothetical protein prgL
CC204_RS21780 3372..3539 - 168 WP_335673682 CHAP domain-containing protein -
CC204_RS21650 3481..4935 - 1455 WP_227011297 phage tail tip lysozyme prgK
CC204_RS21655 4964..5986 - 1023 WP_227011298 hypothetical protein prgK
CC204_RS20610 5998..8380 - 2383 Protein_9 TraC-F-type conjugal transfer protein -
CC204_RS20615 8334..8687 - 354 WP_088271968 PrgI family mobile element protein prgIc
CC204_RS20620 8689..9489 - 801 WP_088271969 type III secretion system protein PrgH prgHb
CC204_RS20625 9502..9729 - 228 WP_069653744 type III secretion system protein PrgF prgF
CC204_RS20630 9761..10216 - 456 WP_088271970 type III secretion system protein PrgE -
CC204_RS20635 10309..10620 - 312 WP_088271971 hypothetical protein -
CC204_RS20640 10896..11474 + 579 WP_088271972 hypothetical protein -
CC204_RS20645 11612..11845 - 234 WP_088271973 hypothetical protein -
CC204_RS20650 11894..12232 - 339 WP_088271974 hypothetical protein -

Region 2: 49783..60240

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CC204_RS20890 47972..49783 - 1812 WP_088272023 VirD4-like conjugal transfer protein, CD1115 family -
CC204_RS20895 49783..50268 - 486 WP_088272024 DUF3801 domain-containing protein gbs1365
CC204_RS20900 50306..51229 - 924 WP_088272025 hypothetical protein -
CC204_RS20905 51308..51616 - 309 WP_088272026 hypothetical protein -
CC204_RS20910 51634..52938 - 1305 WP_088272027 hypothetical protein -
CC204_RS20915 52931..53200 - 270 WP_088272029 type III secretion system protein PrgM -
CC204_RS20920 53193..53864 - 672 WP_088272031 hypothetical protein prgL
CC204_RS20925 53887..56499 - 2613 WP_088272032 phage tail tip lysozyme prgK
CC204_RS20930 56511..58892 - 2382 WP_088272034 VirB4-like conjugal transfer ATPase, CD1110 family virb4
CC204_RS21660 58986..59198 - 213 WP_227011299 PrgI family protein prgIc
CC204_RS20940 59200..60000 - 801 WP_088271969 type III secretion system protein PrgH prgHb
CC204_RS20945 60013..60240 - 228 WP_069653744 type III secretion system protein PrgF prgF
CC204_RS20950 60272..60727 - 456 WP_088271970 type III secretion system protein PrgE -
CC204_RS20955 60821..61132 - 312 WP_088271971 hypothetical protein -
CC204_RS20960 61407..61706 + 300 WP_088272035 hypothetical protein -
CC204_RS21755 62224..62352 - 129 WP_257790127 hypothetical protein -
CC204_RS20965 62378..62737 - 360 WP_088272037 hypothetical protein -
CC204_RS20970 62920..63099 - 180 WP_088272038 hypothetical protein -
CC204_RS20975 63113..63604 - 492 WP_088272040 SspB-related isopeptide-forming adhesin -
CC204_RS20980 63739..63960 - 222 WP_088272041 LPXTG cell wall anchor domain-containing protein -
CC204_RS21435 63954..64252 - 299 WP_193434227 hypothetical protein -

Host bacterium

ID   3324 GenBank   NZ_CP021878
Plasmid name   MN05|unnamed5 Incompatibility group   -
Plasmid size   64252 bp Coordinate of oriT [Strand]   43193..43242 [-]
Host baterium   Enterococcus wangshanyuanii strain MN05

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA2