Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102880
Name   oriT_pKp145/11a in_silico
Organism   Klebsiella pneumoniae strain 145
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX154765 (40364..40463 [+], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pKp145/11a
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure



Relaxase

ID   2159 GenBank   WP_012561142
Name   TrwC_BA903_RS00660_pKp145/11a insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   868 GenBank   WP_001749975
Name   WP_001749975_pKp145/11a insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   1829 GenBank   WP_000342688
Name   t4cp2_BA903_RS00665_pKp145/11a insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11921..22250

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA903_RS00485 6973..8406 - 1434 WP_016338383 DNA cytosine methyltransferase -
BA903_RS00490 8515..8721 - 207 WP_001749967 hypothetical protein -
BA903_RS00495 8726..9112 - 387 WP_045325216 restriction endonuclease -
BA903_RS00500 9163..10131 + 969 WP_016151349 IS5 family transposase -
BA903_RS34015 10173..10433 - 261 WP_016359294 hypothetical protein -
BA903_RS00510 10489..10800 - 312 WP_013279382 hypothetical protein -
BA903_RS00515 10836..11150 - 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
BA903_RS00520 11147..11491 - 345 WP_012561155 hypothetical protein -
BA903_RS00525 11507..11884 - 378 WP_193567606 H-NS family nucleoid-associated regulatory protein -
BA903_RS00530 11921..12658 + 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
BA903_RS00535 12667..12948 + 282 WP_016338364 transcriptional repressor KorA -
BA903_RS00540 12958..13251 + 294 WP_001749962 hypothetical protein virB2
BA903_RS00545 13302..13619 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BA903_RS00550 13619..16219 + 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BA903_RS00555 16237..16950 + 714 WP_001749960 type IV secretion system protein virB5
BA903_RS00560 16958..17185 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
BA903_RS00565 17201..18241 + 1041 WP_001749958 type IV secretion system protein virB6
BA903_RS34625 18342..18470 + 129 WP_071882546 conjugal transfer protein TraN -
BA903_RS00575 18460..19158 + 699 WP_000646594 type IV secretion system protein virB8
BA903_RS00580 19169..20053 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
BA903_RS00585 20053..21213 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
BA903_RS00590 21255..22250 + 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
BA903_RS33225 22250..22354 + 105 Protein_26 phospholipase D family protein -
BA903_RS00595 22706..24025 + 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
BA903_RS00600 24275..25156 - 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
BA903_RS00610 25543..26322 - 780 WP_004152394 IS21-like element ISKpn7 family helper ATPase IstB -

Host bacterium

ID   3323 GenBank   NZ_KX154765
Plasmid name   pKp145/11a Incompatibility group   IncN
Plasmid size   50800 bp Coordinate of oriT [Strand]   40364..40463 [+]
Host baterium   Klebsiella pneumoniae strain 145

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -