Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102876
Name   oriT_pKP148,PINH-4900 in_silico
Organism   Klebsiella pneumoniae strain 148
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX062091 (12738..12837 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pKP148,PINH-4900
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2155 GenBank   WP_012561142
Name   TrwC_A9Z49_RS00110_pKP148,PINH-4900 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   865 GenBank   WP_001749975
Name   WP_001749975_pKP148,PINH-4900 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   1824 GenBank   WP_000342688
Name   t4cp2_A9Z49_RS00105_pKP148,PINH-4900 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 30951..41280

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z49_RS00160 26879..27658 + 780 WP_004152394 IS21-like element ISKpn7 family helper ATPase IstB -
A9Z49_RS00170 28045..28926 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
A9Z49_RS00175 29176..30495 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
A9Z49_RS28775 30847..30951 - 105 Protein_32 phospholipase D family protein -
A9Z49_RS00180 30951..31946 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
A9Z49_RS00185 31988..33148 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
A9Z49_RS00190 33148..34032 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
A9Z49_RS00195 34043..34741 - 699 WP_000646594 type IV secretion system protein virB8
A9Z49_RS29780 34731..34889 - 159 WP_012561180 hypothetical protein -
A9Z49_RS00205 34960..36000 - 1041 WP_001749958 type IV secretion system protein virB6
A9Z49_RS00210 36016..36243 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
A9Z49_RS00215 36251..36964 - 714 WP_001749960 type IV secretion system protein virB5
A9Z49_RS00220 36982..39582 - 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
A9Z49_RS00225 39582..39899 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
A9Z49_RS00230 39950..40243 - 294 WP_001749962 hypothetical protein virB2
A9Z49_RS00235 40253..40534 - 282 WP_016338364 transcriptional repressor KorA -
A9Z49_RS00240 40543..41280 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
A9Z49_RS00245 41323..41694 + 372 WP_223174968 H-NS family nucleoid-associated regulatory protein -
A9Z49_RS00250 41710..42054 + 345 WP_012561155 hypothetical protein -
A9Z49_RS00255 42051..42365 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
A9Z49_RS00260 42401..42712 + 312 WP_013279382 hypothetical protein -
A9Z49_RS29420 42768..43028 + 261 WP_016359294 hypothetical protein -
A9Z49_RS00270 43070..44038 - 969 WP_016151349 IS5 family transposase -
A9Z49_RS00275 44122..44475 + 354 WP_225622145 restriction endonuclease -
A9Z49_RS00280 44480..44686 + 207 WP_001749967 hypothetical protein -
A9Z49_RS00285 44697..44968 - 272 Protein_54 IS1 family transposase -

Host bacterium

ID   3319 GenBank   NZ_KX062091
Plasmid name   pKP148,PINH-4900 Incompatibility group   IncN
Plasmid size   52536 bp Coordinate of oriT [Strand]   12738..12837 [-]
Host baterium   Klebsiella pneumoniae strain 148

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -