Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102868
Name   oriT_pFAM21845_2 in_silico
Organism   Escherichia coli strain FAM21845
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP017222 (45501..45849 [-], 349 nt)
oriT length   349 nt
IRs (inverted repeats)      134..140, 154..160  (AAAATAT..ATATTTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 349 nt

>oriT_pFAM21845_2
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2145 GenBank   WP_000539538
Name   Relaxase_BHT24_RS27090_pFAM21845_2 insolico UniProt ID   A0A5J2A6K9
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44507.83 Da        Isoelectric Point: 10.5067

>WP_000539538.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKDHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(70-229)


  Protein structure


Source ID Structure
AlphaFold DB A0A5J2A6K9


T4CP

ID   1812 GenBank   WP_236922165
Name   t4cp2_BHT24_RS26805_pFAM21845_2 insolico UniProt ID   _
Length   664 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 664 a.a.        Molecular weight: 75509.82 Da        Isoelectric Point: 5.7310

>WP_236922165.1 conjugal transfer protein [Escherichia coli]
MNEICDRLNTYLSRFHSRRLGLYEDHGVVYSDQLSLFQYLLSGRWQKVRVSSSPFYTYLGGKDLFFGNDA
GQITASDHARYFRCIEIKDYFQETDAGILDALMYLPVEYVVTSSFTAMDKQSAIKALDDQIDKLEMTDDA
AKSLLADLKVGLDMVSSGYISFGKSHQTLVVFADSPERLVKDTNIVTSTLEDLGLIVTYSTLSLGAAYFA
QLPGNYTLRPRLSTLSSLNFAEMESFHNFFSGKEKGNTWGEKLITLRGSGNDIYHLNYHMTTEHQNFFGK
NPTLGHTEILGTSNVGKTVLLMTKAFAAQQFGTPESFPANRKLKKLTTVFFDKDRAGEVGIRAMGGSYYR
VKEGEPTGWNPAALPPTKRNIAFMKDLVRLLCTLNSEPLDDYQNSLISDAVERLMQRSDRSYPISKLRPL
IQEPDDTETKRHGLKARLKPWTQGEEFGWVFDNREDTFDVDNLDVFGIDGTEFLDNKVLASAASFYLIYR
VTMLADGRRLLIYMDEFWQWINNEAFRDFVYNKLKTGRKLDMVLVVATQSPDELIKSPIAAAVREQCATH
IYLANPKAKRSEYVDGLQVRELYFDKIKAIDPLSRQFLVVKNPQRKGESDDFAAFARLELGKAAYYLPVL
SASKPQLELFDDIWKEGMKPEEWLDTYLEQANLI

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(19-221)

  Protein structure



No available structure.



ID   1813 GenBank   WP_014106755
Name   t4cp2_BHT24_RS26845_pFAM21845_2 insolico UniProt ID   _
Length   611 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 611 a.a.        Molecular weight: 69401.28 Da        Isoelectric Point: 7.1491

>WP_014106755.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRIDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(102-571)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 869..10829

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT24_RS26785 176..694 + 519 WP_001446885 transcription termination/antitermination NusG family protein -
BHT24_RS26790 (BHT24_25545) 624..836 + 213 WP_000125172 hypothetical protein -
BHT24_RS26795 (BHT24_25550) 869..1516 + 648 WP_000953526 lytic transglycosylase domain-containing protein virB1
BHT24_RS26800 (BHT24_25555) 1494..1790 + 297 WP_000916182 TrbC/VirB2 family protein virB2
BHT24_RS26805 (BHT24_25560) 1809..4562 + 2754 WP_001352869 VirB3 family type IV secretion system protein -
BHT24_RS26810 (BHT24_25565) 4573..5331 + 759 WP_000744200 type IV secretion system protein -
BHT24_RS26815 (BHT24_25570) 5332..5559 + 228 WP_000835349 EexN family lipoprotein -
BHT24_RS26820 (BHT24_25575) 5569..6702 + 1134 WP_000513457 type IV secretion system protein virB6
BHT24_RS26825 (BHT24_25580) 6947..7660 + 714 WP_000394615 type IV secretion system protein virB8
BHT24_RS26830 (BHT24_25585) 7665..8594 + 930 WP_000783379 TrbG/VirB9 family P-type conjugative transfer protein virB9
BHT24_RS26835 (BHT24_25590) 8591..9796 + 1206 WP_000139139 VirB10/TraB/TrbI family type IV secretion system protein virB10
BHT24_RS26840 (BHT24_25595) 9798..10829 + 1032 WP_001058465 P-type DNA transfer ATPase VirB11 virB11
BHT24_RS26845 (BHT24_25600) 10832..12667 + 1836 WP_014106755 type IV secretory system conjugative DNA transfer family protein -
BHT24_RS26850 (BHT24_25605) 12664..13077 + 414 WP_000722603 cag pathogenicity island Cag12 family protein -
BHT24_RS26855 (BHT24_25610) 13074..13376 + 303 WP_000717624 TrbM/KikA/MpfK family conjugal transfer protein -
BHT24_RS26860 (BHT24_25615) 13491..14414 - 924 WP_000902513 SMEK domain-containing protein -

Host bacterium

ID   3311 GenBank   NZ_CP017222
Plasmid name   pFAM21845_2 Incompatibility group   IncX1
Plasmid size   54159 bp Coordinate of oriT [Strand]   45501..45849 [-]
Host baterium   Escherichia coli strain FAM21845

Cargo genes

Drug resistance gene   blaTEM-1A
Virulence gene   mrkA, mrkB, mrkC, mrkD, mrkF
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -