Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102867
Name   oriT_pFAM21845_1 in_silico
Organism   Escherichia coli strain FAM21845
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP017221 (98473..98824 [-], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_pFAM21845_1
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGAGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGCTACAACAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCATTATGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTCAAAAAAGGTAATCTCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2144 GenBank   WP_000986969
Name   traI_BHT24_RS26255_pFAM21845_1 insolico UniProt ID   A0A731Y7R8
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191917.73 Da        Isoelectric Point: 5.9718

>WP_000986969.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
APVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVQIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

AAA domain (AAA_30)

(969-1156)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




Auxiliary protein

ID   863 GenBank   WP_223195105
Name   WP_223195105_pFAM21845_1 insolico UniProt ID   _
Length   133 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 133 a.a.        Molecular weight: 15423.73 Da        Isoelectric Point: 9.9240

>WP_223195105.1 MULTISPECIES: conjugal transfer relaxosome DNA-bindin protein TraY [Enterobacteriaceae]
MLLKRFGTRSATGKMVKLKLPLDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFNTL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(18-61)

TraY domain (TraY)

(72-119)


  Protein structure



No available structure.



ID   864 GenBank   WP_001151528
Name   WP_001151528_pFAM21845_1 insolico UniProt ID   A0A731UEV6
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14577.51 Da        Isoelectric Point: 5.0517

>WP_001151528.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LEYVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A731UEV6


T4CP

ID   1810 GenBank   WP_000009324
Name   traD_BHT24_RS26270_pFAM21845_1 insolico UniProt ID   _
Length   717 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 717 a.a.        Molecular weight: 81528.14 Da        Isoelectric Point: 5.1126

>WP_000009324.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEITGGRQLTDNPKEVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQKMQRREEVN
INVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   1811 GenBank   WP_001064265
Name   traC_BHT24_RS26360_pFAM21845_1 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99296.07 Da        Isoelectric Point: 6.3032

>WP_001064265.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKNRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
AGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

Family of unknown function (DUF5934) (DUF5934)

(289-446)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 72937..99395

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT24_RS26260 (BHT24_25070) 72303..72530 + 228 WP_000450520 toxin-antitoxin system antitoxin VapB -
BHT24_RS26265 (BHT24_25075) 72530..72928 + 399 WP_000911313 type II toxin-antitoxin system VapC family toxin -
BHT24_RS26270 (BHT24_25080) 72937..75090 - 2154 WP_000009324 type IV conjugative transfer system coupling protein TraD virb4
BHT24_RS26275 (BHT24_25085) 75343..76074 - 732 WP_023565183 conjugal transfer complement resistance protein TraT -
BHT24_RS26280 (BHT24_25090) 76106..76603 - 498 WP_000605859 entry exclusion protein -
BHT24_RS26285 (BHT24_25095) 76619..79441 - 2823 WP_001007016 conjugal transfer mating-pair stabilization protein TraG traG
BHT24_RS26290 (BHT24_25100) 79438..80811 - 1374 WP_001517790 conjugal transfer pilus assembly protein TraH traH
BHT24_RS26295 (BHT24_25105) 80811..81191 - 381 WP_001366201 F-type conjugal transfer protein TrbF -
BHT24_RS26300 (BHT24_25110) 81178..81519 - 342 WP_001443031 P-type conjugative transfer protein TrbJ -
BHT24_RS26305 (BHT24_25115) 81449..81994 - 546 WP_000059851 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
BHT24_RS26310 (BHT24_25120) 81981..82265 - 285 WP_000624109 type-F conjugative transfer system pilin chaperone TraQ -
BHT24_RS26315 (BHT24_25125) 82346..82660 + 315 WP_000415566 protein ArtA -
BHT24_RS26320 (BHT24_25130) 82662..83009 - 348 WP_001287910 conjugal transfer protein TrbA -
BHT24_RS26325 (BHT24_25135) 83025..83768 - 744 WP_001030378 type-F conjugative transfer system pilin assembly protein TraF traF
BHT24_RS26330 (BHT24_25140) 83761..84018 - 258 WP_000864318 conjugal transfer protein TrbE -
BHT24_RS26335 (BHT24_25145) 84045..85853 - 1809 WP_000821840 type-F conjugative transfer system mating-pair stabilization protein TraN traN
BHT24_RS26340 (BHT24_25150) 85850..86488 - 639 WP_000777690 type-F conjugative transfer system pilin assembly protein TrbC trbC
BHT24_RS26345 (BHT24_25155) 86497..87489 - 993 WP_000830835 conjugal transfer pilus assembly protein TraU traU
BHT24_RS26350 (BHT24_25160) 87486..88118 - 633 WP_001203750 type-F conjugative transfer system protein TraW traW
BHT24_RS26355 (BHT24_25165) 88115..88501 - 387 WP_000099693 type-F conjugative transfer system protein TrbI -
BHT24_RS26360 (BHT24_25170) 88498..91125 - 2628 WP_001064265 type IV secretion system protein TraC virb4
BHT24_RS26365 91285..91506 - 222 WP_001278686 conjugal transfer protein TraR -
BHT24_RS26370 (BHT24_25175) 91641..92156 - 516 WP_000809885 type IV conjugative transfer system lipoprotein TraV traV
BHT24_RS26375 (BHT24_25180) 92153..92404 - 252 WP_001038331 conjugal transfer protein TrbG -
BHT24_RS26380 (BHT24_25185) 92397..92717 - 321 WP_001057304 conjugal transfer protein TrbD virb4
BHT24_RS26385 (BHT24_25190) 92704..93264 - 561 WP_000896605 conjugal transfer pilus-stabilizing protein TraP -
BHT24_RS26390 (BHT24_25195) 93254..94705 - 1452 WP_000146659 F-type conjugal transfer pilus assembly protein TraB traB
BHT24_RS26395 (BHT24_25200) 94705..95433 - 729 WP_001230805 type-F conjugative transfer system secretin TraK traK
BHT24_RS26400 (BHT24_25205) 95420..95986 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
BHT24_RS26405 (BHT24_25210) 96008..96319 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
BHT24_RS26410 (BHT24_25215) 96324..96686 - 363 WP_000994784 type IV conjugative transfer system pilin TraA -
BHT24_RS26415 (BHT24_25220) 96719..97114 - 396 WP_001366159 conjugal transfer relaxosome DNA-bindin protein TraY -
BHT24_RS26420 (BHT24_25225) 97213..97902 - 690 WP_000283383 conjugal transfer transcriptional regulator TraJ -
BHT24_RS26425 (BHT24_25230) 98089..98472 - 384 WP_001151528 conjugal transfer relaxosome DNA-binding protein TraM -
BHT24_RS26430 (BHT24_25235) 98793..99395 + 603 WP_000243710 transglycosylase SLT domain-containing protein virB1
BHT24_RS26435 (BHT24_25240) 99692..100513 - 822 WP_001234423 DUF932 domain-containing protein -
BHT24_RS26440 (BHT24_25245) 100633..100919 - 287 Protein_117 hypothetical protein -
BHT24_RS28460 (BHT24_25250) 100944..101150 - 207 WP_015059635 hypothetical protein -
BHT24_RS28465 101220..101393 + 174 Protein_119 hypothetical protein -
BHT24_RS28685 101391..101620 - 230 Protein_120 hypothetical protein -
BHT24_RS26465 (BHT24_25260) 101840..101965 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
BHT24_RS28470 101907..102056 - 150 Protein_122 plasmid maintenance protein Mok -
BHT24_RS27940 (BHT24_25275) 103524..103712 + 189 WP_001299721 hypothetical protein -

Host bacterium

ID   3310 GenBank   NZ_CP017221
Plasmid name   pFAM21845_1 Incompatibility group   IncFIB
Plasmid size   147225 bp Coordinate of oriT [Strand]   98473..98824 [-]
Host baterium   Escherichia coli strain FAM21845

Cargo genes

Drug resistance gene   tet(B), aph(3'')-Ib, aph(6)-Id, aph(3')-Ia, aph(4)-Ia, aac(3)-IVa, dfrA1, ant(3'')-Ia, qacE, sul1, blaTEM-1A, sitABCD
Virulence gene   iutA, iucC, iucB, iucA
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -