Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102854 |
| Name | oriT_pATCC10717  |
| Organism | Salmonella enterica subsp. enterica serovar Rubislaw str. ATCC 10717 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP019193 (167346..167427 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pATCC10717
GGGACAGGATATGTTTTGTTGAACCGCCTGCACGCAGTCGGCTTCAAAAAACGTCCTGGTCAGGGCGAAGCCCCGACACCCC
GGGACAGGATATGTTTTGTTGAACCGCCTGCACGCAGTCGGCTTCAAAAAACGTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2126 | GenBank | WP_023208061 |
| Name | Relaxase_SEERU717_RS23560_pATCC10717 |
UniProt ID | A0A6W0P431 |
| Length | 663 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 663 a.a. Molecular weight: 75998.24 Da Isoelectric Point: 6.1901
>WP_023208061.1 TraI/MobA(P) family conjugative relaxase [Salmonella enterica]
MIVIVPEKRRDGKSSFLTLVSYLTLREDVRLAEPVSPDAPVMRMSRSKEAIFDRLVDYIDRSQNAVTQTP
VKEFADGRSQVLSGNVACETNCFSLDTASAEMNVVAAQNTRCVDPVYHFILSWPAQDNPVDADIFDCARS
AIQALGMKEHQFVTAIHRDTDHVHCHVAVNRINPISYRAANLYNDIDTLHKVCRLLELKYDYTPENGAWV
NENGHVVRNTNDVKSIPRKARQLEYHSDKESLYSYAVNECRERIGEILGSGEASWEKLHAELIRAGLDMR
EKGEGLAIYSRHDENVTPIKASTLHPDLTRQCLEKWLGDFEPSPEVVNNFDEDGVYLGSNYPQEFVYDAR
AHVRDQGARSERRLARAVAREDLDARYKAYKNAWVRPRMPDTDSGVQFRALSNRYAWKKEQVRYVFDDPL
IRKLIYRTLELERQKEFEMLRDKVKAEKTQFYSRPENRRLSYWQWVERQAAAQDQAAISRLRGRAYKLKQ
KQRTPGLSENAIVCAVADDIAMVNIDGFESRVNRDGVIQYLQDGHVVIQDSGERIEIADPYREDGIHISD
AMLIAEEKSGEKLVFSGDGRFVNSACDVVQWFNEGGSNPLPLTDPQQRVRACYERPESRTEQPVFTHRIM
DEETYLASLKREDVAASDDRHKPEPGKNTYRPG
MIVIVPEKRRDGKSSFLTLVSYLTLREDVRLAEPVSPDAPVMRMSRSKEAIFDRLVDYIDRSQNAVTQTP
VKEFADGRSQVLSGNVACETNCFSLDTASAEMNVVAAQNTRCVDPVYHFILSWPAQDNPVDADIFDCARS
AIQALGMKEHQFVTAIHRDTDHVHCHVAVNRINPISYRAANLYNDIDTLHKVCRLLELKYDYTPENGAWV
NENGHVVRNTNDVKSIPRKARQLEYHSDKESLYSYAVNECRERIGEILGSGEASWEKLHAELIRAGLDMR
EKGEGLAIYSRHDENVTPIKASTLHPDLTRQCLEKWLGDFEPSPEVVNNFDEDGVYLGSNYPQEFVYDAR
AHVRDQGARSERRLARAVAREDLDARYKAYKNAWVRPRMPDTDSGVQFRALSNRYAWKKEQVRYVFDDPL
IRKLIYRTLELERQKEFEMLRDKVKAEKTQFYSRPENRRLSYWQWVERQAAAQDQAAISRLRGRAYKLKQ
KQRTPGLSENAIVCAVADDIAMVNIDGFESRVNRDGVIQYLQDGHVVIQDSGERIEIADPYREDGIHISD
AMLIAEEKSGEKLVFSGDGRFVNSACDVVQWFNEGGSNPLPLTDPQQRVRACYERPESRTEQPVFTHRIM
DEETYLASLKREDVAASDDRHKPEPGKNTYRPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6W0P431 |
T4CP
| ID | 1789 | GenBank | WP_023208038 |
| Name | trbC_SEERU717_RS23435_pATCC10717 |
UniProt ID | _ |
| Length | 731 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 731 a.a. Molecular weight: 82968.43 Da Isoelectric Point: 7.7677
>WP_023208038.1 F-type conjugative transfer protein TrbC [Salmonella enterica]
MTTGNTQPVDKQRVRQITQSTWIDDYLLAPATVQAGLVVILVLTFLRTWILLPGVFATSIWLLTFFGQLF
RMPLRMPKDIGGFDMTTERENALEFKGPMGLFRFTRRRLTWEKSAGIMCLGHARRRFLGRELWLTRDDCL
RHMQLLATTGSGKTEALLSLQLNSLCMGRGMMFSDGKAETKLAYAIWSLMRRYGQEDNYYVLNFLNGGRD
RFDELLGNDRTRPQSNSINFFGDATATFIIQLMESLLPQVGSNEAGWQDKAKPMLYGLVYALYYKCRKEN
IRLSQSMIQKHLPLKEMADLYIEAKQNGWHDEAVSALEAYLSNLAGFRMELVSKSSEWDQGVYDQHGFLT
QQFSRMLAMFNDVYGHIFSSDAGDIDLSDVLHNDRTLVVLIPALELSKSESANLGKLYISAKRMVIARDL
GYQLEGKSRDVLTSAKFYNPFPFPDVHDELGSYFAPGMDDLAAQMRSLGVMLVISAQDIQRFVAQFKGEY
QTVNANTLVKWFMAMQDEKDTFELAKATAGKDYYAELGEMKRTPGTVSSSYEEANTTYIREKNRITLDEL
KDLNPGEGFISFKSALVPGSAIYIPDSEKISSKLSLRINRFIDTGNPTEADLVAQNPHLRRLLPPSELEV
SMLLQRTDEIIAGSETHTLPPLMDPILARLMKVAMDLDNRCDISYTPTQRGILLFEAARDVLKKRGKKWF
TVKTQPNQLRVSDAMAHRLARKSTAFMPLEQ
MTTGNTQPVDKQRVRQITQSTWIDDYLLAPATVQAGLVVILVLTFLRTWILLPGVFATSIWLLTFFGQLF
RMPLRMPKDIGGFDMTTERENALEFKGPMGLFRFTRRRLTWEKSAGIMCLGHARRRFLGRELWLTRDDCL
RHMQLLATTGSGKTEALLSLQLNSLCMGRGMMFSDGKAETKLAYAIWSLMRRYGQEDNYYVLNFLNGGRD
RFDELLGNDRTRPQSNSINFFGDATATFIIQLMESLLPQVGSNEAGWQDKAKPMLYGLVYALYYKCRKEN
IRLSQSMIQKHLPLKEMADLYIEAKQNGWHDEAVSALEAYLSNLAGFRMELVSKSSEWDQGVYDQHGFLT
QQFSRMLAMFNDVYGHIFSSDAGDIDLSDVLHNDRTLVVLIPALELSKSESANLGKLYISAKRMVIARDL
GYQLEGKSRDVLTSAKFYNPFPFPDVHDELGSYFAPGMDDLAAQMRSLGVMLVISAQDIQRFVAQFKGEY
QTVNANTLVKWFMAMQDEKDTFELAKATAGKDYYAELGEMKRTPGTVSSSYEEANTTYIREKNRITLDEL
KDLNPGEGFISFKSALVPGSAIYIPDSEKISSKLSLRINRFIDTGNPTEADLVAQNPHLRRLLPPSELEV
SMLLQRTDEIIAGSETHTLPPLMDPILARLMKVAMDLDNRCDISYTPTQRGILLFEAARDVLKKRGKKWF
TVKTQPNQLRVSDAMAHRLARKSTAFMPLEQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 106323..144719
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SEERU717_RS23215 (SEERU717_23230) | 101420..102412 | + | 993 | WP_023207997 | toxin co-regulated pilus biosynthesis Q family protein | - |
| SEERU717_RS23220 (SEERU717_23235) | 102412..102843 | + | 432 | WP_023207998 | type IV pilus biogenesis protein PilM | - |
| SEERU717_RS23225 (SEERU717_23240) | 102858..104480 | + | 1623 | WP_023207999 | PilN family type IVB pilus formation outer membrane protein | - |
| SEERU717_RS23230 (SEERU717_23245) | 104485..105768 | + | 1284 | WP_023208000 | type 4b pilus protein PilO2 | - |
| SEERU717_RS23235 (SEERU717_23250) | 105911..106291 | + | 381 | WP_256386068 | type IV pilus biogenesis protein PilP | - |
| SEERU717_RS23240 (SEERU717_23255) | 106323..107822 | + | 1500 | WP_023208002 | ATPase, T2SS/T4P/T4SS family | virB11 |
| SEERU717_RS23245 (SEERU717_23260) | 107815..108930 | + | 1116 | WP_023208003 | type II secretion system F family protein | - |
| SEERU717_RS23250 (SEERU717_23265) | 108957..109550 | + | 594 | WP_023208004 | type 4 pilus major pilin | - |
| SEERU717_RS23255 (SEERU717_23270) | 109564..110034 | + | 471 | WP_023208005 | lytic transglycosylase domain-containing protein | virB1 |
| SEERU717_RS23260 (SEERU717_23275) | 110034..110645 | + | 612 | WP_023208006 | prepilin peptidase | - |
| SEERU717_RS23265 (SEERU717_23280) | 110744..112078 | + | 1335 | WP_023208007 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| SEERU717_RS23270 (SEERU717_23285) | 112134..112640 | + | 507 | WP_050582076 | DapH/DapD/GlmU-related protein | - |
| SEERU717_RS25155 (SEERU717_23290) | 113042..113296 | + | 255 | Protein_82 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| SEERU717_RS24210 (SEERU717_23295) | 113380..113766 | - | 387 | Protein_83 | phage tail protein | - |
| SEERU717_RS24705 | 114040..114354 | - | 315 | WP_138021619 | hypothetical protein | - |
| SEERU717_RS23290 (SEERU717_23305) | 115066..116220 | + | 1155 | WP_023208010 | site-specific integrase | - |
| SEERU717_RS23295 (SEERU717_23310) | 116339..116794 | + | 456 | WP_023208011 | DotD/TraH family lipoprotein | - |
| SEERU717_RS23300 (SEERU717_23315) | 116791..117597 | + | 807 | WP_033904336 | type IV secretory system conjugative DNA transfer family protein | traI |
| SEERU717_RS23305 (SEERU717_23320) | 117594..118763 | + | 1170 | WP_023208013 | plasmid transfer ATPase TraJ | virB11 |
| SEERU717_RS23310 (SEERU717_23325) | 118753..119019 | + | 267 | WP_023208014 | IcmT/TraK family protein | traK |
| SEERU717_RS23315 (SEERU717_23330) | 119031..122504 | + | 3474 | WP_023208015 | DUF5710 domain-containing protein | - |
| SEERU717_RS23320 (SEERU717_23335) | 122520..122990 | + | 471 | WP_023208016 | hypothetical protein | traL |
| SEERU717_RS23325 (SEERU717_23340) | 123054..123731 | + | 678 | WP_023208017 | DotI/IcmL/TraM family protein | traM |
| SEERU717_RS23330 (SEERU717_23345) | 123728..124747 | + | 1020 | WP_023208018 | DotH/IcmK family type IV secretion protein | traN |
| SEERU717_RS23335 (SEERU717_23350) | 124747..125946 | + | 1200 | WP_023208019 | conjugal transfer protein TraO | traO |
| SEERU717_RS23340 (SEERU717_23355) | 126042..126695 | + | 654 | WP_138021618 | hypothetical protein | traP |
| SEERU717_RS23345 (SEERU717_23360) | 126705..127235 | + | 531 | WP_023208021 | conjugal transfer protein TraQ | traQ |
| SEERU717_RS23350 (SEERU717_23365) | 127251..127649 | + | 399 | WP_023208022 | DUF6750 family protein | traR |
| SEERU717_RS23355 (SEERU717_23370) | 127682..128257 | - | 576 | WP_023208023 | hypothetical protein | - |
| SEERU717_RS23360 (SEERU717_23375) | 128515..128949 | + | 435 | WP_023208024 | hypothetical protein | - |
| SEERU717_RS23365 (SEERU717_23380) | 129036..129644 | + | 609 | WP_023208025 | hypothetical protein | traT |
| SEERU717_RS23370 (SEERU717_23385) | 129641..132703 | + | 3063 | WP_023208026 | hypothetical protein | traU |
| SEERU717_RS23375 (SEERU717_23390) | 132722..133948 | + | 1227 | WP_024146665 | conjugal transfer protein TraW | traW |
| SEERU717_RS23380 (SEERU717_23395) | 133945..134463 | + | 519 | WP_023208028 | conjugal transfer protein TraX | - |
| SEERU717_RS23385 (SEERU717_23400) | 134515..136662 | + | 2148 | WP_023208029 | DotA/TraY family protein | traY |
| SEERU717_RS23390 (SEERU717_23405) | 136670..137164 | + | 495 | WP_023208030 | hypothetical protein | - |
| SEERU717_RS23395 (SEERU717_23410) | 137264..137779 | - | 516 | WP_023208031 | GNAT family N-acetyltransferase | - |
| SEERU717_RS23400 (SEERU717_23415) | 137763..138056 | - | 294 | WP_000987497 | DUF1778 domain-containing protein | - |
| SEERU717_RS24845 | 138145..138303 | - | 159 | WP_001644829 | hypothetical protein | - |
| SEERU717_RS23410 (SEERU717_23425) | 138527..139180 | + | 654 | WP_023208032 | DUF5710 domain-containing protein | - |
| SEERU717_RS25225 | 139563..139658 | + | 96 | WP_000247681 | DinQ-like type I toxin DqlB | - |
| SEERU717_RS23415 (SEERU717_23430) | 139755..139919 | - | 165 | WP_023208033 | DUF1127 domain-containing protein | - |
| SEERU717_RS23420 (SEERU717_23435) | 140098..141510 | + | 1413 | WP_023208034 | PLP-dependent aminotransferase family protein | - |
| SEERU717_RS25230 | 142089..142184 | + | 96 | WP_023208035 | DinQ-like type I toxin DqlB | - |
| SEERU717_RS23425 (SEERU717_23440) | 142328..143644 | + | 1317 | WP_024146667 | hypothetical protein | trbA |
| SEERU717_RS23430 (SEERU717_23445) | 143697..144719 | + | 1023 | WP_023208037 | thioredoxin fold domain-containing protein | trbB |
| SEERU717_RS23435 (SEERU717_23450) | 144706..146901 | + | 2196 | WP_023208038 | F-type conjugative transfer protein TrbC | - |
| SEERU717_RS23440 (SEERU717_23455) | 146962..147501 | + | 540 | WP_033904337 | phospholipase D family protein | - |
| SEERU717_RS23445 (SEERU717_23460) | 147596..148171 | + | 576 | WP_023208040 | DUF2913 family protein | - |
| SEERU717_RS23450 (SEERU717_23465) | 148357..148611 | + | 255 | WP_023208041 | TfoX/Sxy family protein | - |
| SEERU717_RS23455 (SEERU717_23470) | 148712..149512 | + | 801 | WP_024146669 | hypothetical protein | - |
Host bacterium
| ID | 3297 | GenBank | NZ_CP019193 |
| Plasmid name | pATCC10717 | Incompatibility group | IncFIB |
| Plasmid size | 196866 bp | Coordinate of oriT [Strand] | 167346..167427 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Rubislaw str. ATCC 10717 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |