Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102830
Name   oriT_MKU-A9-MCR|unnamed2 in_silico
Organism   Escherichia coli strain MKU-A9-MCR
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QBRW01000025 (49771..49894 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      101..106, 119..124  (TTTAAT..ATTAAA)
 91..99, 113..121  (AATAATGTA..TACATTATT)
 90..95, 107..112  (AAATAA..TTATTT)
 88..93, 105..110  (ATAAAT..ATTTAT)
 41..48, 61..68  (AAAAACAA..TTGTTTTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_MKU-A9-MCR|unnamed2
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGTTTTTTGAATCATTAGCTTATGTTATAAATAATGTATTTTAATTTATTTTACATTATTAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2098 GenBank   WP_011251384
Name   traI_DB282_RS21575_MKU-A9-MCR|unnamed2 insolico UniProt ID   Q5JBP8
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191818.65 Da        Isoelectric Point: 6.0139

>WP_011251384.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGINVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




Auxiliary protein

ID   846 GenBank   WP_001254385
Name   WP_001254385_MKU-A9-MCR|unnamed2 insolico UniProt ID   A0A1Q9L2K4
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 8975.08 Da        Isoelectric Point: 10.1422

>WP_001254385.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Enterobacteriaceae]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVAEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A1Q9L2K4

ID   847 GenBank   WP_000124979
Name   WP_000124979_MKU-A9-MCR|unnamed2 insolico UniProt ID   Q5JBL6
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14456.50 Da        Isoelectric Point: 5.2973

>WP_000124979.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECAVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDGE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-125)


  Protein structure


Source ID Structure
AlphaFold DB Q5JBL6


T4CP

ID   1759 GenBank   WP_021518883
Name   traD_DB282_RS21580_MKU-A9-MCR|unnamed2 insolico UniProt ID   _
Length   726 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 726 a.a.        Molecular weight: 82493.06 Da        Isoelectric Point: 5.0504

>WP_021518883.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ
PQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQ
QMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   1760 GenBank   WP_011251375
Name   traC_DB282_RS21660_MKU-A9-MCR|unnamed2 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99370.02 Da        Isoelectric Point: 5.9655

>WP_011251375.1 type IV secretion system protein TraC [Escherichia coli]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAA
ATQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQY
TAGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-447)

TraG P-loop domain (P-loop_TraG)

(468-772)

TraC protein (TraC_F_IV)

(39-277)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 23709..50466

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DB282_RS21580 (DB282_21580) 23709..25889 - 2181 WP_021518883 type IV conjugative transfer system coupling protein TraD virb4
DB282_RS21585 (DB282_21585) 26142..26873 - 732 WP_000850424 conjugal transfer complement resistance protein TraT -
DB282_RS21590 (DB282_21590) 26905..27408 - 504 WP_000605510 hypothetical protein -
DB282_RS21595 (DB282_21595) 27423..30245 - 2823 WP_011251382 conjugal transfer mating-pair stabilization protein TraG traG
DB282_RS21600 (DB282_21600) 30242..31615 - 1374 WP_001137358 conjugal transfer pilus assembly protein TraH traH
DB282_RS21605 (DB282_21605) 31615..31977 - 363 WP_011251381 P-type conjugative transfer protein TrbJ -
DB282_RS21610 (DB282_21610) 31907..32452 - 546 WP_000052618 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
DB282_RS21615 (DB282_21615) 32439..32723 - 285 WP_000624110 type-F conjugative transfer system pilin chaperone TraQ -
DB282_RS21620 (DB282_21620) 32842..33189 - 348 WP_011251380 conjugal transfer protein TrbA -
DB282_RS21625 (DB282_21625) 33205..33948 - 744 WP_001030390 type-F conjugative transfer system pilin assembly protein TraF traF
DB282_RS21630 (DB282_21630) 33941..34198 - 258 WP_000864328 conjugal transfer protein TrbE -
DB282_RS21635 (DB282_21635) 34225..36033 - 1809 WP_011251379 type-F conjugative transfer system mating-pair stabilization protein TraN traN
DB282_RS21640 (DB282_21640) 36030..36668 - 639 WP_000777692 type-F conjugative transfer system pilin assembly protein TrbC trbC
DB282_RS21645 (DB282_21645) 36677..37669 - 993 WP_001712363 conjugal transfer pilus assembly protein TraU traU
DB282_RS21650 (DB282_21650) 37666..38298 - 633 WP_011251377 type-F conjugative transfer system protein TraW traW
DB282_RS21655 (DB282_21655) 38295..38681 - 387 WP_011251376 type-F conjugative transfer system protein TrbI -
DB282_RS21660 (DB282_21660) 38678..41308 - 2631 WP_011251375 type IV secretion system protein TraC virb4
DB282_RS27710 41434..41796 - 363 WP_000836686 hypothetical protein -
DB282_RS21670 (DB282_21670) 41824..42039 - 216 WP_000556749 hypothetical protein -
DB282_RS21675 (DB282_21675) 42119..42592 - 474 WP_000549595 hypothetical protein -
DB282_RS21680 (DB282_21680) 42585..42758 - 174 WP_024262213 conjugal transfer protein TraR -
DB282_RS21685 (DB282_21685) 42876..43391 - 516 WP_000809838 type IV conjugative transfer system lipoprotein TraV traV
DB282_RS21690 (DB282_21690) 43388..43639 - 252 WP_001038341 conjugal transfer protein TrbG -
DB282_RS21695 (DB282_21695) 43636..43953 - 318 WP_001057292 conjugal transfer protein TrbD virb4
DB282_RS21700 (DB282_21700) 43950..44522 - 573 WP_000002794 conjugal transfer pilus-stabilizing protein TraP -
DB282_RS21705 (DB282_21705) 44512..45939 - 1428 WP_000146685 F-type conjugal transfer pilus assembly protein TraB traB
DB282_RS21710 (DB282_21710) 45939..46667 - 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
DB282_RS21715 (DB282_21715) 46654..47220 - 567 WP_011251373 type IV conjugative transfer system protein TraE traE
DB282_RS21720 (DB282_21720) 47242..47553 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
DB282_RS21725 (DB282_21725) 47568..47927 - 360 WP_000340272 type IV conjugative transfer system pilin TraA -
DB282_RS21730 (DB282_21730) 47961..48188 - 228 WP_001254385 conjugal transfer relaxosome protein TraY -
DB282_RS21735 (DB282_21735) 48282..48968 - 687 WP_000332493 PAS domain-containing protein -
DB282_RS21740 (DB282_21740) 49159..49542 - 384 WP_000124979 conjugal transfer relaxosome DNA-binding protein TraM -
DB282_RS21750 (DB282_21750) 49819..50466 + 648 WP_000615259 transglycosylase SLT domain-containing protein virB1
DB282_RS21755 (DB282_21755) 50763..51584 - 822 WP_001234469 DUF932 domain-containing protein -
DB282_RS21760 (DB282_21760) 51707..51994 - 288 WP_000107535 hypothetical protein -
DB282_RS21765 (DB282_21765) 52019..52225 - 207 WP_000547971 hypothetical protein -
DB282_RS28500 52295..52468 + 174 Protein_69 hypothetical protein -
DB282_RS28505 (DB282_21775) 52466..52696 - 231 WP_001426396 hypothetical protein -
DB282_RS21780 (DB282_21780) 52915..53040 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
DB282_RS28510 52982..53131 - 150 Protein_72 plasmid maintenance protein Mok -
DB282_RS28040 53153..53341 + 189 WP_001299721 hypothetical protein -
DB282_RS21785 (DB282_21785) 53310..54072 - 763 Protein_74 plasmid SOS inhibition protein A -
DB282_RS21790 (DB282_21790) 54069..54503 - 435 WP_000845873 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   3273 GenBank   NZ_QBRW01000025
Plasmid name   MKU-A9-MCR|unnamed2 Incompatibility group   IncFII
Plasmid size   64150 bp Coordinate of oriT [Strand]   49771..49894 [-]
Host baterium   Escherichia coli strain MKU-A9-MCR

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -