Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102824
Name   oriT_pKp_Goe_414-4 in_silico
Organism   Klebsiella pneumoniae isolate Kp_Goe_154414
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP018341 (14847..14928 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pKp_Goe_414-4
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2092 GenBank   WP_032440569
Name   Relaxase_BB788_RS29795_pKp_Goe_414-4 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   1751 GenBank   WP_032440593
Name   trbC_BB788_RS30135_pKp_Goe_414-4 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 65446..81243

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB788_RS30125 (BB788_29840) 61209..61904 - 696 WP_254910806 RepA family replication protein -
BB788_RS32120 62410..62661 - 252 WP_113394424 hypothetical protein -
BB788_RS30130 (BB788_29845) 62673..63206 - 534 WP_032440594 phospholipase D family protein -
BB788_RS30135 (BB788_29850) 63252..65444 - 2193 WP_032440593 F-type conjugative transfer protein TrbC -
BB788_RS30140 (BB788_29855) 65446..66537 - 1092 WP_050597336 thioredoxin fold domain-containing protein trbB
BB788_RS30145 (BB788_29860) 66548..67966 - 1419 WP_063666705 hypothetical protein trbA
BB788_RS30150 (BB788_29865) 68068..68691 - 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
BB788_RS30155 (BB788_29870) 68757..70901 - 2145 WP_032440591 DotA/TraY family protein traY
BB788_RS30160 (BB788_29875) 70943..71524 - 582 WP_050597335 conjugal transfer protein TraX -
BB788_RS30165 (BB788_29880) 71521..72717 - 1197 WP_032440590 conjugal transfer protein TraW traW
BB788_RS30170 (BB788_29885) 72727..75774 - 3048 WP_032440589 conjugal transfer protein traU
BB788_RS32125 75784..76347 - 564 WP_045325070 hypothetical protein traT
BB788_RS30180 (BB788_29895) 76391..76792 - 402 WP_032440588 DUF6750 family protein traR
BB788_RS30185 (BB788_29900) 76848..77381 - 534 WP_032440587 conjugal transfer protein TraQ traQ
BB788_RS30190 (BB788_29905) 77391..78110 - 720 WP_050597333 conjugal transfer protein TraP traP
BB788_RS30195 (BB788_29910) 78117..79457 - 1341 WP_032440586 conjugal transfer protein TraO traO
BB788_RS30200 (BB788_29915) 79461..80564 - 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
BB788_RS30205 (BB788_29920) 80575..81243 - 669 WP_032440585 DotI/IcmL/TraM family protein traM

Host bacterium

ID   3267 GenBank   NZ_CP018341
Plasmid name   pKp_Goe_414-4 Incompatibility group   IncFII
Plasmid size   81641 bp Coordinate of oriT [Strand]   14847..14928 [-]
Host baterium   Klebsiella pneumoniae isolate Kp_Goe_154414

Cargo genes

Drug resistance gene   qnrS1, blaLAP-2, tet(A), catA2, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -