Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102820 |
| Name | oriT_pKp_Goe_304-2  |
| Organism | Klebsiella pneumoniae strain KP_Goe_828304 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018722 (71419..71569 [-], 151 nt) |
| oriT length | 151 nt |
| IRs (inverted repeats) | 93..98, 106..111 (TGGCCT..AGGCCA) 12..17, 24..29 (AACCCT..AGGGTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 151 nt
>oriT_pKp_Goe_304-2
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2089 | GenBank | WP_001326173 |
| Name | TraI_2_BB787_RS29930_pKp_Goe_304-2 |
UniProt ID | F5BPR1 |
| Length | 990 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | F5BPR1 |
T4CP
| ID | 1748 | GenBank | WP_000178857 |
| Name | traD_BB787_RS29925_pKp_Goe_304-2 |
UniProt ID | _ |
| Length | 621 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 621 a.a. Molecular weight: 69225.71 Da Isoelectric Point: 6.9403
>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 64395..73485
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB787_RS33515 | 60021..60218 | + | 198 | Protein_78 | mercuric resistance transcriptional repressor MerD | - |
| BB787_RS29825 (BB787_29825) | 60272..60976 | - | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| BB787_RS29830 (BB787_29830) | 61041..61214 | + | 174 | Protein_80 | transcriptional regulator MerD | - |
| BB787_RS29835 (BB787_29835) | 61211..61447 | + | 237 | WP_001087810 | broad-spectrum mercury transporter MerE | - |
| BB787_RS29840 (BB787_29840) | 61483..62118 | + | 636 | Protein_82 | EAL domain-containing protein | - |
| BB787_RS33520 | 62230..62433 | + | 204 | WP_024198696 | DUF3330 domain-containing protein | - |
| BB787_RS29845 (BB787_29845) | 62559..63095 | + | 537 | WP_029404405 | recombinase family protein | - |
| BB787_RS29860 (BB787_29860) | 63146..63843 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB787_RS29865 (BB787_29865) | 63999..64391 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| BB787_RS29870 (BB787_29870) | 64395..64973 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| BB787_RS29875 (BB787_29875) | 64970..66283 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| BB787_RS29880 (BB787_29880) | 66283..67200 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| BB787_RS29885 (BB787_29885) | 67184..67810 | - | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| BB787_RS29890 (BB787_29890) | 67807..68088 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| BB787_RS29895 (BB787_29895) | 68233..68598 | - | 366 | WP_001052530 | hypothetical protein | - |
| BB787_RS33220 | 68610..68753 | - | 144 | WP_001275801 | hypothetical protein | - |
| BB787_RS29900 (BB787_29900) | 68934..69596 | - | 663 | WP_001231464 | hypothetical protein | - |
| BB787_RS29905 (BB787_29905) | 69596..69973 | - | 378 | WP_000869297 | hypothetical protein | - |
| BB787_RS29910 (BB787_29910) | 69983..70429 | - | 447 | WP_000122507 | hypothetical protein | - |
| BB787_RS29915 (BB787_29915) | 70439..71068 | - | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| BB787_RS29920 (BB787_29920) | 71025..71570 | - | 546 | WP_000228720 | hypothetical protein | - |
| BB787_RS29925 (BB787_29925) | 71620..73485 | - | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| BB787_RS29930 (BB787_29930) | 73482..76454 | - | 2973 | WP_001326173 | MobH family relaxase | - |
| BB787_RS29935 (BB787_29935) | 76622..77239 | + | 618 | WP_001249395 | hypothetical protein | - |
| BB787_RS29940 (BB787_29940) | 77221..77454 | + | 234 | WP_001191890 | hypothetical protein | - |
Host bacterium
| ID | 3263 | GenBank | NZ_CP018722 |
| Plasmid name | pKp_Goe_304-2 | Incompatibility group | IncA/C2 |
| Plasmid size | 96073 bp | Coordinate of oriT [Strand] | 71419..71569 [-] |
| Host baterium | Klebsiella pneumoniae strain KP_Goe_828304 |
Cargo genes
| Drug resistance gene | floR, sul1, qacE, aac(6')-Ib, sul2, aph(3'')-Ib, aph(6)-Id, tet(A) |
| Virulence gene | - |
| Metal resistance gene | merR, merT, merP, merA, merB, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |