Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102816
Name   oriT_FWSEC0375|unnamed1 in_silico
Organism   Escherichia coli strain FWSEC0375
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRMZ01000118 (51888..51973 [+], 86 nt)
oriT length   86 nt
IRs (inverted repeats)      61..68, 73..80  (TTGGTGGT..ACCACCAA)
 27..34, 37..44  (GCAAAAAC..GTTTTTGC)
 8..13, 21..26  (TGATTT..AAATCA)
Location of nic site      53..54
Conserved sequence flanking the
  nic site    		  TGTGTGGTGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 86 nt

>oriT_FWSEC0375|unnamed1
AATTGCATGATTTAAAACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2085 GenBank   WP_136767366
Name   traI_C9151_RS25345_FWSEC0375|unnamed1 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191427.05 Da        Isoelectric Point: 5.8823

>WP_136767366.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
AGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAILTQAIRSELKTQDVLGHQEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGEMQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGEASLESAISHQKSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLHVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSLSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQQTRVIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVL
EPGADREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(968-1156)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-627)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1434-1557)


  Protein structure



No available structure.




Auxiliary protein

ID   840 GenBank   WP_000089265
Name   WP_000089265_FWSEC0375|unnamed1 insolico UniProt ID   A0A1E5M428
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 8472.68 Da        Isoelectric Point: 9.9218

>WP_000089265.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Enterobacteriaceae]
MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVRLRDHLSRFPDFYNLDAIKEGTGETDS
IIKDL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-60)


  Protein structure


Source ID Structure
AlphaFold DB A0A1E5M428

ID   841 GenBank   WP_001457391
Name   WP_001457391_FWSEC0375|unnamed1 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14520.52 Da        Isoelectric Point: 5.0277

>WP_001457391.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM, partial [Escherichia]
MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLELGLRVYEAQMERKESAFNQKEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDNE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-125)


  Protein structure



No available structure.




T4CP

ID   1743 GenBank   WP_123129820
Name   traD_C9151_RS25350_FWSEC0375|unnamed1 insolico UniProt ID   _
Length   735 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 735 a.a.        Molecular weight: 83772.54 Da        Isoelectric Point: 5.1071

>WP_123129820.1 type IV conjugative transfer system coupling protein TraD [Escherichia coli]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPVFPVINDKKSDTGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVMDMAVYEAWQ
REQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   1744 GenBank   WP_136767370
Name   traC_C9151_RS25465_FWSEC0375|unnamed1 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99420.10 Da        Isoelectric Point: 5.6262

>WP_136767370.1 type IV secretion system protein TraC [Escherichia coli]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDSTMGFMLEAI
PINGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAA
ETLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFRQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERIRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(39-277)

TraG P-loop domain (P-loop_TraG)

(468-771)

Family of unknown function (DUF5934) (DUF5934)

(290-447)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 22201..52541

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9151_RS25350 (C9151_25410) 22201..24408 - 2208 WP_123129820 type IV conjugative transfer system coupling protein TraD virb4
C9151_RS25355 (C9151_25415) 24465..25196 - 732 WP_136767367 hypothetical protein -
C9151_RS25360 (C9151_25420) 25364..25624 - 261 WP_001077900 hypothetical protein -
C9151_RS25365 (C9151_25425) 25684..26415 - 732 WP_000782448 conjugal transfer complement resistance protein TraT -
C9151_RS25370 (C9151_25430) 26464..26943 - 480 WP_024210214 surface exclusion protein -
C9151_RS25375 (C9151_25435) 26965..29808 - 2844 WP_136767368 conjugal transfer mating-pair stabilization protein TraG traG
C9151_RS25380 (C9151_25440) 29805..31178 - 1374 WP_000944332 conjugal transfer pilus assembly protein TraH traH
C9151_RS25385 (C9151_25445) 31165..31557 - 393 WP_024213667 F-type conjugal transfer protein TrbF -
C9151_RS25390 (C9151_25450) 31538..31885 - 348 WP_071531747 P-type conjugative transfer protein TrbJ -
C9151_RS25395 (C9151_25455) 31815..32360 - 546 WP_001477377 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
C9151_RS25400 (C9151_25460) 32347..32634 - 288 WP_000335419 type-F conjugative transfer system pilin chaperone TraQ -
C9151_RS25405 (C9151_25465) 32700..33101 - 402 WP_024213666 hypothetical protein -
C9151_RS25410 (C9151_25470) 33232..33597 - 366 WP_136767369 hypothetical protein -
C9151_RS25415 (C9151_25475) 33611..34354 - 744 WP_024213664 type-F conjugative transfer system pilin assembly protein TraF traF
C9151_RS25420 (C9151_25480) 34347..34604 - 258 WP_024213663 conjugal transfer protein TrbE -
C9151_RS25425 (C9151_25485) 34618..36522 - 1905 WP_047081608 type-F conjugative transfer system mating-pair stabilization protein TraN traN
C9151_RS25430 (C9151_25490) 36616..36996 - 381 WP_000056343 hypothetical protein -
C9151_RS25435 (C9151_25495) 37022..37660 - 639 WP_001562875 type-F conjugative transfer system pilin assembly protein TrbC trbC
C9151_RS25440 (C9151_25500) 37853..38092 + 240 WP_001066128 hypothetical protein -
C9151_RS25445 (C9151_25505) 38089..38268 - 180 WP_000970212 hypothetical protein -
C9151_RS25450 (C9151_25510) 38289..39281 - 993 WP_000817328 conjugal transfer pilus assembly protein TraU traU
C9151_RS25455 (C9151_25515) 39278..39910 - 633 WP_000877291 type-F conjugative transfer system protein TraW traW
C9151_RS25460 (C9151_25520) 39907..40293 - 387 WP_000214090 type-F conjugative transfer system protein TrbI -
C9151_RS25465 (C9151_25525) 40290..42920 - 2631 WP_136767370 type IV secretion system protein TraC virb4
C9151_RS25470 (C9151_25530) 43045..43392 - 348 WP_000836684 hypothetical protein -
C9151_RS27870 (C9151_25535) 43420..43638 - 219 WP_071528664 hypothetical protein -
C9151_RS25480 (C9151_25540) 43718..44191 - 474 WP_032327008 hypothetical protein -
C9151_RS25485 (C9151_25545) 44202..44597 - 396 WP_000549593 hypothetical protein -
C9151_RS25490 (C9151_25550) 44590..44811 - 222 WP_001278977 conjugal transfer protein TraR -
C9151_RS25495 (C9151_25555) 44946..45461 - 516 WP_000809868 type IV conjugative transfer system lipoprotein TraV traV
C9151_RS25500 (C9151_25560) 45458..45709 - 252 WP_001038341 conjugal transfer protein TrbG -
C9151_RS25505 (C9151_25565) 45702..46022 - 321 WP_001057270 conjugal transfer protein TrbD virb4
C9151_RS25510 (C9151_25570) 46009..46596 - 588 WP_021521307 conjugal transfer pilus-stabilizing protein TraP -
C9151_RS25515 (C9151_25575) 46586..48016 - 1431 WP_136767371 F-type conjugal transfer pilus assembly protein TraB traB
C9151_RS25520 (C9151_25580) 48016..48744 - 729 WP_001365587 type-F conjugative transfer system secretin TraK traK
C9151_RS25525 (C9151_25585) 48731..49297 - 567 WP_000399759 type IV conjugative transfer system protein TraE traE
C9151_RS25530 (C9151_25590) 49319..49630 - 312 WP_000012096 type IV conjugative transfer system protein TraL traL
C9151_RS25535 (C9151_25595) 49645..50004 - 360 WP_000991521 type IV conjugative transfer system pilin TraA -
C9151_RS25540 (C9151_25600) 50037..50264 - 228 WP_000089265 conjugal transfer relaxosome protein TraY -
C9151_RS25545 (C9151_25605) 50389..51042 - 654 WP_001562879 hypothetical protein -
C9151_RS25550 (C9151_25610) 51233..51616 - 384 WP_001457391 conjugal transfer relaxosome DNA-binding protein TraM -
C9151_RS25560 (C9151_25620) 51951..52541 + 591 WP_000252679 transglycosylase SLT domain-containing protein virB1
C9151_RS25565 (C9151_25625) 52838..53659 - 822 WP_001234463 DUF932 domain-containing protein -
C9151_RS25570 (C9151_25630) 53778..54065 - 288 WP_000107526 hypothetical protein -
C9151_RS25580 (C9151_25640) 54221..54523 - 303 WP_001272235 hypothetical protein -
C9151_RS27365 (C9151_25650) 54840..55119 + 280 Protein_75 hypothetical protein -
C9151_RS25595 (C9151_25655) 55419..55541 - 123 WP_223200913 Hok/Gef family protein -
C9151_RS27875 55486..55638 - 153 Protein_77 DUF5431 family protein -
C9151_RS25600 (C9151_25660) 55788..56102 - 315 WP_000872086 hypothetical protein -
C9151_RS25605 (C9151_25665) 56099..56454 - 356 WP_162780471 plasmid SOS inhibition protein A -

Host bacterium

ID   3259 GenBank   NZ_RRMZ01000118
Plasmid name   FWSEC0375|unnamed1 Incompatibility group   -
Plasmid size   56454 bp Coordinate of oriT [Strand]   51888..51973 [+]
Host baterium   Escherichia coli strain FWSEC0375

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -