Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102791
Name   oriT_pUCLAOXA232-3 in_silico
Organism   Klebsiella pneumoniae strain UCLAOXA232KP
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP012564 (54084..54165 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pUCLAOXA232-3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2063 GenBank   WP_015632446
Name   Relaxase_AM277_RS28240_pUCLAOXA232-3 insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   1721 GenBank   WP_015632442
Name   trbC_AM277_RS28275_pUCLAOXA232-3 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62026..83867

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM277_RS28255 57086..57493 + 408 WP_015632445 transposase -
AM277_RS28260 (AM277_27080) 57490..57839 + 350 Protein_74 IS66 family insertion sequence element accessory protein TnpB -
AM277_RS28265 (AM277_27085) 57869..59458 + 1590 WP_015632444 IS66 family transposase -
AM277_RS28270 (AM277_27090) 59481..59786 - 306 WP_015632443 phospholipase D-like domain-containing protein -
AM277_RS28275 (AM277_27095) 59832..62024 - 2193 WP_015632442 F-type conjugative transfer protein TrbC -
AM277_RS28280 62026..63117 - 1092 WP_050484136 thioredoxin fold domain-containing protein trbB
AM277_RS28285 (AM277_27105) 63128..64546 - 1419 WP_015632440 hypothetical protein trbA
AM277_RS28290 (AM277_27110) 64648..65271 - 624 WP_015632439 plasmid IncI1-type surface exclusion protein ExcA -
AM277_RS28295 (AM277_27115) 65337..67481 - 2145 WP_015632438 DotA/TraY family protein traY
AM277_RS28300 67523..68104 - 582 WP_015632437 conjugal transfer protein TraX -
AM277_RS28305 (AM277_27125) 68101..69297 - 1197 WP_015632436 conjugal transfer protein TraW traW
AM277_RS28310 (AM277_27130) 69308..72355 - 3048 WP_015632435 conjugal transfer protein traU
AM277_RS32035 72365..72679 - 315 WP_123677146 hypothetical protein traT
AM277_RS28320 (AM277_27140) 72972..73373 - 402 WP_015632433 DUF6750 family protein traR
AM277_RS28325 (AM277_27145) 73427..73960 - 534 WP_015632432 conjugal transfer protein TraQ traQ
AM277_RS28330 (AM277_27150) 73970..74689 - 720 WP_015632431 conjugal transfer protein TraP traP
AM277_RS28335 (AM277_27155) 74695..76035 - 1341 WP_015632430 conjugal transfer protein TraO traO
AM277_RS28340 (AM277_27160) 76039..77142 - 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
AM277_RS28345 (AM277_27165) 77153..77857 - 705 WP_015632428 DotI/IcmL/TraM family protein traM
AM277_RS28350 (AM277_27170) 77893..78246 - 354 WP_015632427 hypothetical protein traL
AM277_RS32700 78280..81603 - 3324 WP_032495745 LPD7 domain-containing protein -
AM277_RS28360 (AM277_27185) 81621..81887 - 267 WP_032441926 IcmT/TraK family protein traK
AM277_RS28365 (AM277_27190) 81884..83071 - 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
AM277_RS28370 (AM277_27195) 83049..83867 - 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
AM277_RS28375 (AM277_27200) 83864..84331 - 468 WP_015632424 DotD/TraH family lipoprotein -
AM277_RS28380 (AM277_27205) 84354..84833 - 480 WP_015632423 lytic transglycosylase domain-containing protein -
AM277_RS28385 (AM277_27210) 85531..85842 - 312 WP_015632421 hypothetical protein -
AM277_RS32040 85876..86079 - 204 WP_032495743 hypothetical protein -
AM277_RS28390 (AM277_27215) 86097..86564 - 468 WP_032495742 transcription termination/antitermination NusG family protein -
AM277_RS28395 (AM277_27220) 86898..87476 + 579 WP_015632419 hypothetical protein -
AM277_RS28400 (AM277_27225) 87458..87928 + 471 WP_032441927 hypothetical protein -
AM277_RS28405 (AM277_27230) 87928..88314 + 387 WP_015632417 acyl-CoA thioesterase -

Host bacterium

ID   3234 GenBank   NZ_CP012564
Plasmid name   pUCLAOXA232-3 Incompatibility group   IncFII
Plasmid size   88800 bp Coordinate of oriT [Strand]   54084..54165 [+]
Host baterium   Klebsiella pneumoniae strain UCLAOXA232KP

Cargo genes

Drug resistance gene   ARR-3, rmtF, blaCTX-M-15
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -