Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102787 |
| Name | oriT_pUCLAOXA232-3.X  |
| Organism | Klebsiella pneumoniae strain UCLAOXA232KP |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP012569 (49014..49095 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pUCLAOXA232-3.X
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2059 | GenBank | WP_015632446 |
| Name | Relaxase_AM278_RS27920_pUCLAOXA232-3.X |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 1716 | GenBank | WP_015632442 |
| Name | trbC_AM278_RS27955_pUCLAOXA232-3.X |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 56956..78797
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM278_RS27935 | 52016..52423 | + | 408 | WP_015632445 | transposase | - |
| AM278_RS27940 (AM278_26790) | 52420..52769 | + | 350 | Protein_70 | IS66 family insertion sequence element accessory protein TnpB | - |
| AM278_RS27945 (AM278_26795) | 52799..54388 | + | 1590 | WP_015632444 | IS66 family transposase | - |
| AM278_RS27950 (AM278_26800) | 54411..54716 | - | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| AM278_RS27955 (AM278_26805) | 54762..56954 | - | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| AM278_RS27960 | 56956..58047 | - | 1092 | WP_050484136 | thioredoxin fold domain-containing protein | trbB |
| AM278_RS27965 (AM278_26815) | 58058..59476 | - | 1419 | WP_015632440 | hypothetical protein | trbA |
| AM278_RS27970 (AM278_26820) | 59578..60201 | - | 624 | WP_015632439 | plasmid IncI1-type surface exclusion protein ExcA | - |
| AM278_RS27975 (AM278_26825) | 60267..62411 | - | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| AM278_RS27980 | 62453..63034 | - | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| AM278_RS27985 (AM278_26835) | 63031..64227 | - | 1197 | WP_015632436 | conjugal transfer protein TraW | traW |
| AM278_RS27990 (AM278_26840) | 64238..67285 | - | 3048 | WP_015632435 | conjugal transfer protein | traU |
| AM278_RS31360 | 67295..67609 | - | 315 | WP_123677146 | hypothetical protein | traT |
| AM278_RS28000 (AM278_26850) | 67902..68303 | - | 402 | WP_015632433 | DUF6750 family protein | traR |
| AM278_RS28005 (AM278_26855) | 68357..68890 | - | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| AM278_RS28010 (AM278_26860) | 68900..69619 | - | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| AM278_RS28015 (AM278_26865) | 69625..70965 | - | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| AM278_RS28020 (AM278_26870) | 70969..72072 | - | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| AM278_RS28025 (AM278_26875) | 72083..72787 | - | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| AM278_RS28030 (AM278_26880) | 72823..73176 | - | 354 | WP_015632427 | hypothetical protein | traL |
| AM278_RS32005 | 73210..76533 | - | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| AM278_RS28040 (AM278_26895) | 76551..76817 | - | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| AM278_RS28045 (AM278_26900) | 76814..78001 | - | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| AM278_RS28050 (AM278_26905) | 77979..78797 | - | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| AM278_RS28055 (AM278_26910) | 78794..79261 | - | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| AM278_RS28060 (AM278_26915) | 79284..79763 | - | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| AM278_RS28065 (AM278_26920) | 80461..80772 | - | 312 | WP_015632421 | hypothetical protein | - |
| AM278_RS31365 | 80806..81009 | - | 204 | WP_032495743 | hypothetical protein | - |
| AM278_RS28070 (AM278_26925) | 81027..81494 | - | 468 | WP_032495742 | transcription termination/antitermination NusG family protein | - |
| AM278_RS28075 (AM278_26930) | 81828..82406 | + | 579 | WP_015632419 | hypothetical protein | - |
| AM278_RS28080 (AM278_26935) | 82388..82858 | + | 471 | WP_032441927 | hypothetical protein | - |
| AM278_RS28085 (AM278_26940) | 82858..83244 | + | 387 | WP_015632417 | acyl-CoA thioesterase | - |
Host bacterium
| ID | 3230 | GenBank | NZ_CP012569 |
| Plasmid name | pUCLAOXA232-3.X | Incompatibility group | IncFII |
| Plasmid size | 83730 bp | Coordinate of oriT [Strand] | 49014..49095 [+] |
| Host baterium | Klebsiella pneumoniae strain UCLAOXA232KP |
Cargo genes
| Drug resistance gene | ARR-3, rmtF |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |