Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102785
Name   oriT_pRspN731c in_silico
Organism   Rhizobium sp. N731
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP013604 (211511..211539 [+], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_pRspN731c
AGGGCGCAATATACGTCGCTGTCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2057 GenBank   WP_046977193
Name   traA_AMK02_RS24250_pRspN731c insolico UniProt ID   A0A192TMB6
Length   1560 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1560 a.a.        Molecular weight: 172207.61 Da        Isoelectric Point: 6.9611

>WP_046977193.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium]
MAIMFVRAQVISRGSGRSIVSAAAYRHRARMMDEQAGTSFSYRGGAGELMYEELALPDEIPDWLRSAISG
QSVSKASEVFWNAVDAFETRADAQLARELIIALPEELTRAENITLVREFVRDNLTSKGMIADWVYHDKDG
NPHIHLMTTLRPLTEEGFGAKKVPVLGEDGKPLRVVTPDRPNGKIVYKVWAGDKETMKAWKIAWAETANR
HLALAGHDIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGRELHFAPADLARRQEMADRLLSEPELLLKQ
LGNERSTFDEREIAKALHRYVDDPVDFANIRVRLMASDQLVILKPQEIEAETGKVSEPAMFTTREMLRIE
YDMAQSARVLSERRGFGVSERNVKVAIESVESGDPKTPFRLDAEQVDAVRHVTGDSGIAAVVGLAGAGKS
TLLAAARLAWEGEGHRVIGAALAGKAAEGLQDSSGIKSRTLASWELAWANGRDTLHRGDVLVIDEAGMVA
SQQMARVLKIAEEAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREAWARNASRLFARGE
VEKGLDAYAQQGHLVEAGTREETIDRIVVDWTEARKHAIGRSISEGRDGRLRGDELLVLAHTNDDVRKLN
EALREVMAGDNALGESRSFRTERGARKFAAGDRIIFLENARFLEPRAKHSGPQYVKNGMLGTVVSTGDKR
GGPLLSVLLDNGNKVVFGEDSYRNVDHGYAATIHKSQGATVDRTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAIEDFEAKPEWGRKAGVDHAAGVTGELVETGEAKFRPDDEDADDSPYADIRTDGGAVHRLWGVSLPKA
LEEASIMEGDTVTLRKDGVERVKVQIAVVDEKTGHKHYEEREVDRNVWTASQVETASARQERIERESHRP
ELFNLLVERLSRSGAKTTTLDFESEASYRAHANDFARRRGLDHLSLAAAEMEESLTRRWAWIAAKREQVE
KLWERASVALGFAIERERRVAYNEERSQTMVEATSSDARTASHSVSGASADETRYLIPPATSFVSSVEED
ARLAQLASPAWTEREVILRPLLEKIYRDPDAALVSLNALASDIGVAARRLADDLAAAPGRLGRLRGSELI
VDGRAAREERNLAVAAVKELLPMARAHATEFRRNAERFDLREQTRRAHMSLSIPALSERAMARLMEIEAV
RRQGGDDAYKSAFALAAEDRSVVQEIKAVSEALTARFGWSAFSAKADAIAERNIVERMPEDLTDERRGKL
TRLFEAVRRFAEEQHLAERRDRSKVVAGASVDLETEKGVGKENVPIPPMLAAVTEFKVPVDDEARSRALS
APLYRQQRVALANAATTIWRDPAEVVGKIEELLQKGFAADRIGAAVANDPAAYAALRGSDRLVDRMLASG
RERKEAVAAVPEAAARLRALGAAYANALNSERQAITEERRRMGVAIPALSKAAEEALARLTAEARKDSRG
LRVSAVPLDPGIGREFAAVSRALDERFGRNAIVRGEKDLVSRLPPAQRRAFEAMQERLKVLQQTVRLQSS
EQITAERRQRAASRARGINL

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1330-1420)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1070-1140)

MobA/MobL family (MobA_MobL)

(17-249)

AAA domain (AAA_30)

(391-577)


  Protein structure



No available structure.




T4CP

ID   1713 GenBank   WP_046977192
Name   traG_AMK02_RS24235_pRspN731c insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 70708.95 Da        Isoelectric Point: 9.2487

>WP_046977192.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium]
MGLRGKPHPSLLLVLAPIAVTTITIYIVGWRWPGLAVGMSGRMEYWFLRAAPVLPLMFGPLAGLLTVWAL
PLHRRRPVAMASLLCFLGIAAYYALREFGRLAPTVQARVVPWDRALSYLDMVAVIAAVAGFMAVAVSARI
SVVVPDEIKRARRGIFGDADWLPMAAAGKLFPPDGEIVVGERYRVDKEIIHALPFDVNDRSTWGQGGKAP
LLTYRQDFDSTHMLFFAGSGGYKTTSNVVPTALIYTGPMICLDPSTEVAPMVVEHRTNALGREVMVLDPT
NPIMGFNVLDGIEASKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYEGRRS
LRSLRQIVSEPEPSVLAMLRDIQEHSGSAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSSDIVSGKKDVFLNISASILRSYPGIARVIIGSLINAMVQADGAFQRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNLSAQCGEMTVEVKG
SSRNIGWDTKNNASRRSENVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDQAAK
VNRFVKPVL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(171-634)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 190802..200381

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK02_RS32280 187290..187516 - 227 Protein_180 chaperonin GroEL -
AMK02_RS24110 (AMK02_PC00164) 187810..188730 + 921 WP_016737442 zincin-like metallopeptidase domain-containing protein -
AMK02_RS24115 188841..189023 - 183 WP_016737443 hypothetical protein -
AMK02_RS24120 (AMK02_PC00165) 189792..190163 - 372 WP_007636193 hypothetical protein -
AMK02_RS24125 (AMK02_PC00166) 190262..190798 + 537 WP_016737444 hypothetical protein -
AMK02_RS24130 (AMK02_PC00167) 190802..191470 + 669 WP_016737445 transglycosylase SLT domain-containing protein virB1
AMK02_RS24135 (AMK02_PC00168) 191467..191766 + 300 WP_007636189 TrbC/VirB2 family protein virB2
AMK02_RS24140 (AMK02_PC00169) 191772..192110 + 339 WP_011053369 type IV secretion system protein VirB3 virB3
AMK02_RS24145 (AMK02_PC00170) 192103..194469 + 2367 WP_046977188 VirB4 family type IV secretion system protein virb4
AMK02_RS24150 (AMK02_PC00171) 194466..195167 + 702 WP_016737448 P-type DNA transfer protein VirB5 virB5
AMK02_RS24155 (AMK02_PC00172) 195164..195379 + 216 WP_016737449 EexN family lipoprotein -
AMK02_RS24160 (AMK02_PC00173) 195382..196314 + 933 WP_046977189 type IV secretion system protein virB6
AMK02_RS32285 196356..196637 + 282 WP_016737561 hypothetical protein -
AMK02_RS24165 (AMK02_PC00174) 196639..197310 + 672 WP_046977190 virB8 family protein virB8
AMK02_RS24170 (AMK02_PC00175) 197307..198164 + 858 WP_016737451 P-type conjugative transfer protein VirB9 virB9
AMK02_RS24175 (AMK02_PC00176) 198173..199345 + 1173 WP_016737452 type IV secretion system protein VirB10 virB10
AMK02_RS24180 (AMK02_PC00177) 199353..200381 + 1029 WP_046977191 P-type DNA transfer ATPase VirB11 virB11
AMK02_RS24185 (AMK02_PC00178) 200425..201003 - 579 WP_016737454 PIN domain-containing protein -
AMK02_RS24190 (AMK02_PC00179) 200993..201439 - 447 WP_029532409 hypothetical protein -
AMK02_RS24195 (AMK02_PC00181) 201949..203139 - 1191 WP_016737456 DUF1173 domain-containing protein -
AMK02_RS31415 203450..203709 + 260 Protein_200 toprim domain-containing protein -
AMK02_RS24200 (AMK02_PC00182) 204032..204972 + 941 Protein_201 DUF2493 domain-containing protein -
AMK02_RS24205 (AMK02_PC00184) 205057..205323 + 267 WP_004676093 hypothetical protein -

Host bacterium

ID   3228 GenBank   NZ_CP013604
Plasmid name   pRspN731c Incompatibility group   -
Plasmid size   433219 bp Coordinate of oriT [Strand]   211511..211539 [+]
Host baterium   Rhizobium sp. N731

Cargo genes

Drug resistance gene   -
Virulence gene   hsiC1/vipB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   nifX, nifN, nifE, nifK, nifD, nifH, nifS, nifW, fixA, fixB, fixC, fixX, nifA, nifB, nifZ, nifT, a9174_31165, nodD, nodJ, nodI, nodS, nodC, nodB, nodZ, fixG, fixO, fixN, mLTONO_5203, nodA
Anti-CRISPR   -