Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102779
Name   oriT_FWSEC0249|unnamed1 in_silico
Organism   Escherichia coli strain FWSEC0249
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRIR01000124 (44493..44842 [-], 350 nt)
oriT length   350 nt
IRs (inverted repeats)      253..260, 269..276  (ACCGCTAG..CTAGCGGT)
 190..196, 204..210  (TATAAAA..TTTTATA)
 38..45, 48..55  (GCAAAAAC..GTTTTTGC)
 2..9, 14..21  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 350 nt

>oriT_FWSEC0249|unnamed1
GTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGCTACAACAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCTAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCATTATGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTCAAAAAAGGTAATCTCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2051 GenBank   WP_064577599
Name   traI_C9036_RS26915_FWSEC0249|unnamed1 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192015.77 Da        Isoelectric Point: 5.8534

>WP_064577599.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQYNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DTVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEYSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAREQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAAQERDRAAITEREAALPESVLRESQREREAVREIVRENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

AAA domain (AAA_30)

(969-1156)


  Protein structure



No available structure.




Auxiliary protein

ID   833 GenBank   WP_001369361
Name   WP_001369361_FWSEC0249|unnamed1 insolico UniProt ID   B7LI64
Length   133 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 133 a.a.        Molecular weight: 15409.70 Da        Isoelectric Point: 9.9240

>WP_001369361.1 MULTISPECIES: conjugal transfer relaxosome DNA-bindin protein TraY [Gammaproteobacteria]
MLLKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFNTL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(18-61)

TraY domain (TraY)

(72-119)


  Protein structure


Source ID Structure
AlphaFold DB B7LI64

ID   834 GenBank   WP_001151524
Name   WP_001151524_FWSEC0249|unnamed1 insolico UniProt ID   A0A5W2DT22
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14517.48 Da        Isoelectric Point: 5.0516

>WP_001151524.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A5W2DT22


T4CP

ID   1706 GenBank   WP_136736736
Name   traD_C9036_RS26920_FWSEC0249|unnamed1 insolico UniProt ID   _
Length   723 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 723 a.a.        Molecular weight: 82100.65 Da        Isoelectric Point: 5.1657

>WP_136736736.1 type IV conjugative transfer system coupling protein TraD [Escherichia coli]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQ
RREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   1707 GenBank   WP_001064260
Name   traC_C9036_RS27010_FWSEC0249|unnamed1 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99179.80 Da        Isoelectric Point: 5.8486

>WP_001064260.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 19140..42326

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9036_RS26920 (C9036_26960) 19140..21311 - 2172 WP_136736736 type IV conjugative transfer system coupling protein TraD virb4
C9036_RS26930 (C9036_26970) 21564..22295 - 732 WP_024257478 conjugal transfer complement resistance protein TraT -
C9036_RS26935 (C9036_26975) 22327..22830 - 504 WP_000605510 hypothetical protein -
C9036_RS26940 (C9036_26980) 22845..25667 - 2823 WP_064577595 conjugal transfer mating-pair stabilization protein TraG traG
C9036_RS26945 (C9036_26985) 25664..27037 - 1374 WP_064577593 conjugal transfer pilus assembly protein TraH traH
C9036_RS26950 (C9036_26990) 27024..27416 - 393 WP_000662237 F-type conjugal transfer protein TrbF -
C9036_RS26955 (C9036_26995) 27403..27744 - 342 WP_071849770 P-type conjugative transfer protein TrbJ -
C9036_RS26960 (C9036_27000) 27674..28219 - 546 WP_064577591 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
C9036_RS26965 (C9036_27005) 28206..28442 - 237 WP_000270010 type-F conjugative transfer system pilin chaperone TraQ -
C9036_RS26970 (C9036_27010) 28603..29346 - 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
C9036_RS26975 (C9036_27015) 29339..29596 - 258 WP_053264198 conjugal transfer protein TrbE -
C9036_RS26980 (C9036_27020) 29623..31485 - 1863 WP_249508035 type-F conjugative transfer system mating-pair stabilization protein TraN traN
C9036_RS26985 (C9036_27025) 31721..32101 - 381 WP_000174466 hypothetical protein -
C9036_RS26990 (C9036_27030) 32098..32736 - 639 WP_024233422 type-F conjugative transfer system pilin assembly protein TrbC trbC
C9036_RS26995 (C9036_27035) 32745..33737 - 993 WP_064577589 conjugal transfer pilus assembly protein TraU traU
C9036_RS27000 (C9036_27040) 33734..34366 - 633 WP_001203720 type-F conjugative transfer system protein TraW traW
C9036_RS27005 (C9036_27045) 34363..34749 - 387 WP_000214096 type-F conjugative transfer system protein TrbI -
C9036_RS27010 (C9036_27050) 34746..37373 - 2628 WP_001064260 type IV secretion system protein TraC virb4
C9036_RS27015 (C9036_27055) 37533..37754 - 222 WP_001278689 conjugal transfer protein TraR -
C9036_RS27020 (C9036_27060) 37889..38404 - 516 WP_000724020 type IV conjugative transfer system lipoprotein TraV traV
C9036_RS27025 (C9036_27065) 38404..38721 - 318 WP_024189706 conjugal transfer protein TrbD -
C9036_RS27030 (C9036_27070) 38708..39295 - 588 WP_062898196 conjugal transfer pilus-stabilizing protein TraP -
C9036_RS27035 (C9036_27075) 39285..40712 - 1428 WP_062898195 F-type conjugal transfer pilus assembly protein TraB traB
C9036_RS27040 (C9036_27080) 40712..41440 - 729 WP_001230783 type-F conjugative transfer system secretin TraK traK
C9036_RS27045 (C9036_27085) 41427..41993 - 567 WP_000399794 type IV conjugative transfer system protein TraE traE
C9036_RS27050 (C9036_27090) 42015..42326 - 312 WP_000012126 type IV conjugative transfer system protein TraL traL
C9036_RS27055 (C9036_27095) 42341..42706 - 366 WP_000994782 type IV conjugative transfer system pilin TraA -
C9036_RS27060 (C9036_27100) 42739..43134 - 396 WP_001309237 conjugal transfer relaxosome DNA-bindin protein TraY -
C9036_RS27065 (C9036_27105) 43233..43922 - 690 WP_000283384 conjugal transfer transcriptional regulator TraJ -
C9036_RS27070 (C9036_27110) 44109..44492 - 384 WP_001151524 conjugal transfer relaxosome DNA-binding protein TraM -
C9036_RS27075 (C9036_27115) 44846..45415 + 570 Protein_47 transglycosylase SLT domain-containing protein -
C9036_RS27080 (C9036_27120) 45712..46533 - 822 WP_001234469 DUF932 domain-containing protein -
C9036_RS27085 (C9036_27125) 46639..46935 - 297 WP_001272240 hypothetical protein -
C9036_RS27840 (C9036_27130) 46958..47164 - 207 WP_024167391 hypothetical protein -

Host bacterium

ID   3222 GenBank   NZ_RRIR01000124
Plasmid name   FWSEC0249|unnamed1 Incompatibility group   -
Plasmid size   53026 bp Coordinate of oriT [Strand]   44493..44842 [-]
Host baterium   Escherichia coli strain FWSEC0249

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -