Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102767 |
| Name | oriT_pIncFIB_DHQP1300920  |
| Organism | Klebsiella pneumoniae isolate 11 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016922 (26002..26051 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pIncFIB_DHQP1300920
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1694 | GenBank | WP_043907039 |
| Name | traD_A8C11_RS02360_pIncFIB_DHQP1300920 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85906.85 Da Isoelectric Point: 5.0577
>WP_043907039.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5092..26609
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A8C11_RS01865 (A8C11_01865) | 905..1453 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| A8C11_RS01870 (A8C11_01870) | 1466..2455 | - | 990 | Protein_2 | conjugal transfer protein TraG | - |
| A8C11_RS01875 (A8C11_01875) | 2513..3210 | + | 698 | WP_223174630 | IS1 family transposase | - |
| A8C11_RS01880 (A8C11_01880) | 3239..5092 | - | 1854 | Protein_4 | conjugal transfer mating-pair stabilization protein TraG | - |
| A8C11_RS01885 (A8C11_01885) | 5092..6471 | - | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| A8C11_RS01890 (A8C11_01890) | 6449..6892 | - | 444 | WP_030003485 | F-type conjugal transfer protein TrbF | - |
| A8C11_RS01895 (A8C11_01895) | 6938..7495 | - | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| A8C11_RS01900 (A8C11_01900) | 7467..7706 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| A8C11_RS01905 (A8C11_01905) | 7717..8469 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| A8C11_RS01910 (A8C11_01910) | 8490..8816 | - | 327 | WP_013214030 | hypothetical protein | - |
| A8C11_RS01915 (A8C11_01915) | 8829..9077 | - | 249 | WP_013214029 | hypothetical protein | - |
| A8C11_RS01920 (A8C11_01920) | 9055..9309 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| A8C11_RS01925 (A8C11_01925) | 9341..11296 | - | 1956 | WP_040145764 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| A8C11_RS01930 (A8C11_01930) | 11344..11982 | - | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| A8C11_RS01935 (A8C11_01935) | 11995..12954 | - | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| A8C11_RS30775 (A8C11_01940) | 12998..13624 | - | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| A8C11_RS30780 (A8C11_01945) | 13624..14002 | - | 379 | Protein_17 | type-F conjugative transfer system protein TrbI | - |
| A8C11_RS01950 (A8C11_01950) | 14002..16641 | - | 2640 | WP_004152592 | type IV secretion system protein TraC | virb4 |
| A8C11_RS01955 (A8C11_01955) | 16713..17111 | - | 399 | WP_004153071 | hypothetical protein | - |
| A8C11_RS01960 (A8C11_01960) | 17407..17811 | - | 405 | WP_004152594 | hypothetical protein | - |
| A8C11_RS01965 (A8C11_01965) | 17878..18195 | - | 318 | WP_004152595 | hypothetical protein | - |
| A8C11_RS01970 (A8C11_01970) | 18196..18414 | - | 219 | WP_004171484 | hypothetical protein | - |
| A8C11_RS01975 (A8C11_01975) | 18438..18728 | - | 291 | WP_004152596 | hypothetical protein | - |
| A8C11_RS01980 (A8C11_01980) | 18733..19143 | - | 411 | WP_004152597 | lipase chaperone | - |
| A8C11_RS01985 (A8C11_01985) | 19275..19844 | - | 570 | WP_004152598 | type IV conjugative transfer system lipoprotein TraV | traV |
| A8C11_RS01990 (A8C11_01990) | 19867..20463 | - | 597 | WP_004152599 | conjugal transfer pilus-stabilizing protein TraP | - |
| A8C11_RS01995 (A8C11_01995) | 20456..21880 | - | 1425 | WP_004152600 | F-type conjugal transfer pilus assembly protein TraB | traB |
| A8C11_RS02000 (A8C11_02000) | 21880..22620 | - | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| A8C11_RS02005 (A8C11_02005) | 22607..23173 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| A8C11_RS02010 (A8C11_02010) | 23193..23498 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| A8C11_RS02015 (A8C11_02015) | 23512..23880 | - | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| A8C11_RS02020 (A8C11_02020) | 23934..24305 | - | 372 | WP_004208838 | TraY domain-containing protein | - |
| A8C11_RS28930 | 24389..25084 | - | 696 | WP_004178061 | transcriptional regulator TraJ family protein | - |
| A8C11_RS02030 (A8C11_02030) | 25298..25690 | - | 393 | WP_004178062 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| A8C11_RS02035 (A8C11_02035) | 26124..26609 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| A8C11_RS28935 | 26642..26971 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| A8C11_RS02040 (A8C11_02040) | 27004..27825 | - | 822 | WP_004178064 | DUF932 domain-containing protein | - |
| A8C11_RS02055 (A8C11_02055) | 28659..29072 | - | 414 | WP_004152722 | helix-turn-helix domain-containing protein | - |
| A8C11_RS02060 (A8C11_02060) | 29073..29351 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| A8C11_RS02065 (A8C11_02065) | 29341..29661 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| A8C11_RS02070 (A8C11_02070) | 29742..29966 | - | 225 | WP_004152719 | hypothetical protein | - |
| A8C11_RS02075 (A8C11_02075) | 29977..30189 | - | 213 | WP_004152718 | hypothetical protein | - |
| A8C11_RS02080 (A8C11_02080) | 30250..30606 | - | 357 | WP_004152717 | hypothetical protein | - |
| A8C11_RS02085 (A8C11_02085) | 31242..31592 | - | 351 | WP_004152758 | hypothetical protein | - |
Host bacterium
| ID | 3210 | GenBank | NZ_CP016922 |
| Plasmid name | pIncFIB_DHQP1300920 | Incompatibility group | IncFIB |
| Plasmid size | 103694 bp | Coordinate of oriT [Strand] | 26002..26051 [+] |
| Host baterium | Klebsiella pneumoniae isolate 11 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |