Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102730 |
| Name | oriT_O177:H21|unnamed1  |
| Organism | Escherichia coli strain O177:H21 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016547 (56178..56231 [-], 54 nt) |
| oriT length | 54 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 54 nt
>oriT_O177:H21|unnamed1
CACAAGCATTGTAACATGCCCGGAACGGGCTTGTGTACAAAGCTTATCGTGCCT
CACAAGCATTGTAACATGCCCGGAACGGGCTTGTGTACAAAGCTTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1657 | GenBank | WP_236927839 |
| Name | t4cp2_BB405_RS26025_O177:H21|unnamed1 |
UniProt ID | _ |
| Length | 547 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 547 a.a. Molecular weight: 61853.05 Da Isoelectric Point: 8.7287
>WP_236927839.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSM
VILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKI
AAILIPASDDPIWSDSARNLFVGLGLYLLDKERFHLEQKTKGHNVPDVLVSISAILKTSVPDGGKDLAAW
MGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSI
YLGLTPDALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNL
RFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIGVRDMKISKRSISSGGGK
GGGSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREIITSEFSRPFIANKIIWFEEPEFKRRVDIAR
NNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVMVAGGNVITNPDLDNHDKTDVSE
MDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSM
VILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKI
AAILIPASDDPIWSDSARNLFVGLGLYLLDKERFHLEQKTKGHNVPDVLVSISAILKTSVPDGGKDLAAW
MGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSI
YLGLTPDALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNL
RFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIGVRDMKISKRSISSGGGK
GGGSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREIITSEFSRPFIANKIIWFEEPEFKRRVDIAR
NNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVMVAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 15548..36130
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB405_RS25980 (BB405_25980) | 11433..12647 | - | 1215 | WP_236927835 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BB405_RS25985 (BB405_25985) | 12660..13295 | - | 636 | WP_000934977 | A24 family peptidase | - |
| BB405_RS25990 (BB405_25990) | 13299..13726 | - | 428 | Protein_18 | lytic transglycosylase domain-containing protein | - |
| BB405_RS25995 (BB405_25995) | 13846..14403 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| BB405_RS26000 (BB405_26000) | 14448..15557 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| BB405_RS26005 (BB405_26005) | 15548..17086 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BB405_RS26010 (BB405_26010) | 17111..17605 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| BB405_RS26015 (BB405_26015) | 17589..18899 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| BB405_RS26020 (BB405_26020) | 18950..20592 | - | 1643 | Protein_24 | PilN family type IVB pilus formation outer membrane protein | - |
| BB405_RS26025 (BB405_26025) | 20643..22286 | - | 1644 | WP_236927839 | type IV secretory system conjugative DNA transfer family protein | - |
| BB405_RS26030 (BB405_26030) | 22616..23671 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| BB405_RS26035 (BB405_26035) | 23690..24829 | - | 1140 | WP_000790641 | TrbI/VirB10 family protein | virB10 |
| BB405_RS26040 (BB405_26040) | 24819..25520 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| BB405_RS26045 (BB405_26045) | 25586..26320 | - | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| BB405_RS26055 (BB405_26055) | 26486..28843 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| BB405_RS26060 (BB405_26060) | 28849..29169 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| BB405_RS33175 (BB405_26065) | 29240..29530 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| BB405_RS26070 (BB405_26070) | 29530..30114 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| BB405_RS26075 (BB405_26075) | 30135..30533 | - | 399 | WP_001153668 | hypothetical protein | - |
| BB405_RS26080 (BB405_26080) | 30652..31089 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| BB405_RS26085 (BB405_26085) | 31095..32330 | - | 1236 | WP_021580486 | TcpQ domain-containing protein | - |
| BB405_RS26090 (BB405_26090) | 32333..32632 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BB405_RS26095 (BB405_26095) | 32699..33334 | - | 636 | WP_000835765 | hypothetical protein | - |
| BB405_RS26100 (BB405_26100) | 33415..34173 | + | 759 | WP_224482110 | DUF5710 domain-containing protein | - |
| BB405_RS26105 (BB405_26105) | 34251..34487 | - | 237 | WP_000750964 | EexN family lipoprotein | - |
| BB405_RS26110 (BB405_26110) | 34491..35138 | - | 648 | WP_000653673 | type IV secretion system protein | - |
| BB405_RS26115 (BB405_26115) | 35144..36130 | - | 987 | WP_001028544 | type IV secretion system protein | virB6 |
| BB405_RS26120 (BB405_26120) | 36134..36391 | - | 258 | WP_000739144 | hypothetical protein | - |
| BB405_RS26125 (BB405_26125) | 36670..36927 | - | 258 | WP_000285945 | hypothetical protein | - |
| BB405_RS26130 (BB405_26130) | 36960..37406 | - | 447 | WP_001243164 | hypothetical protein | - |
| BB405_RS32480 | 37417..37587 | - | 171 | WP_000550720 | hypothetical protein | - |
| BB405_RS30095 | 37591..38034 | - | 444 | WP_000964331 | NfeD family protein | - |
| BB405_RS26140 (BB405_26140) | 38408..39361 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| BB405_RS26145 (BB405_26145) | 39407..39601 | - | 195 | WP_001127356 | DUF1187 family protein | - |
| BB405_RS26150 (BB405_26150) | 39594..40046 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 3173 | GenBank | NZ_CP016547 |
| Plasmid name | O177:H21|unnamed1 | Incompatibility group | IncI2 |
| Plasmid size | 86371 bp | Coordinate of oriT [Strand] | 56178..56231 [-] |
| Host baterium | Escherichia coli strain O177:H21 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |