Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102728 |
| Name | oriT_pAMR588-04-00435_37  |
| Organism | Salmonella enterica subsp. enterica serovar Heidelberg strain AMR588-04-00435 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016575 (3808..4414 [-], 607 nt) |
| oriT length | 607 nt |
| IRs (inverted repeats) | 590..595, 600..605 (ATGCCC..GGGCAT) 517..523, 529..535 (CCTCCCG..CGGGAGG) 453..458, 462..467 (GTTCGC..GCGAAC) 196..202, 204..210 (TCAATTC..GAATTGA) 175..180, 187..192 (AAATCA..TGATTT) 103..108, 120..125 (GCTAAA..TTTAGC) 79..84, 93..98 (GCTTTA..TAAAGC) 29..37, 46..54 (TTTTGATAA..TTATCAAAA) |
| Location of nic site | 313..314 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 607 nt
>oriT_pAMR588-04-00435_37
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2008 | GenBank | WP_000539532 |
| Name | Relaxase_BAT64_RS22980_pAMR588-04-00435_37 |
UniProt ID | K4NPN4 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44348.73 Da Isoelectric Point: 10.5588
>WP_000539532.1 MULTISPECIES: MobP1 family relaxase [Gammaproteobacteria]
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | K4NPN4 |
T4CP
| ID | 1656 | GenBank | WP_000053816 |
| Name | t4cp2_BAT64_RS23040_pAMR588-04-00435_37 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69371.31 Da Isoelectric Point: 8.0636
>WP_000053816.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Gammaproteobacteria]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTAVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKNRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTAVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKNRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 6954..16906
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BAT64_RS22955 (BAT64_22945) | 2270..2515 | + | 246 | WP_000356547 | hypothetical protein | - |
| BAT64_RS22960 (BAT64_22950) | 2505..2720 | + | 216 | WP_001180116 | hypothetical protein | - |
| BAT64_RS22965 (BAT64_22955) | 2813..3142 | + | 330 | WP_000866646 | hypothetical protein | - |
| BAT64_RS24540 | 3185..3364 | + | 180 | WP_000439078 | hypothetical protein | - |
| BAT64_RS25250 | 3386..3583 | + | 198 | WP_001675595 | hypothetical protein | - |
| BAT64_RS24545 | 3596..3868 | + | 273 | WP_000160399 | hypothetical protein | - |
| BAT64_RS22975 (BAT64_22965) | 4194..4739 | + | 546 | WP_000757693 | DNA distortion polypeptide 1 | - |
| BAT64_RS22980 (BAT64_22970) | 4742..5908 | + | 1167 | WP_000539532 | MobP1 family relaxase | - |
| BAT64_RS24550 | 6261..6779 | + | 519 | WP_001446885 | transcription termination/antitermination NusG family protein | - |
| BAT64_RS22985 (BAT64_22975) | 6709..6921 | + | 213 | WP_000125172 | hypothetical protein | - |
| BAT64_RS22990 (BAT64_22980) | 6954..7601 | + | 648 | WP_000953530 | lytic transglycosylase domain-containing protein | virB1 |
| BAT64_RS22995 (BAT64_22985) | 7579..7875 | + | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| BAT64_RS23000 (BAT64_22990) | 7894..10647 | + | 2754 | WP_001532172 | VirB3 family type IV secretion system protein | virb4 |
| BAT64_RS23005 (BAT64_22995) | 10658..11407 | + | 750 | WP_000744206 | type IV secretion system protein | - |
| BAT64_RS23010 (BAT64_23000) | 11422..11706 | + | 285 | WP_000748128 | EexN family lipoprotein | - |
| BAT64_RS23015 (BAT64_23005) | 11718..12779 | + | 1062 | WP_000796671 | type IV secretion system protein | virB6 |
| BAT64_RS23020 (BAT64_23010) | 13024..13737 | + | 714 | WP_000394613 | type IV secretion system protein | virB8 |
| BAT64_RS23025 (BAT64_23015) | 13742..14671 | + | 930 | WP_000783379 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| BAT64_RS23030 (BAT64_23020) | 14668..15873 | + | 1206 | WP_000139138 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| BAT64_RS23035 (BAT64_23025) | 15875..16906 | + | 1032 | WP_001058474 | P-type DNA transfer ATPase VirB11 | virB11 |
| BAT64_RS23040 (BAT64_23030) | 16909..18744 | + | 1836 | WP_000053816 | type IV secretory system conjugative DNA transfer family protein | - |
| BAT64_RS23045 (BAT64_23035) | 18741..19154 | + | 414 | WP_000722610 | cag pathogenicity island Cag12 family protein | - |
| BAT64_RS23050 (BAT64_23040) | 19151..19453 | + | 303 | WP_000820909 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BAT64_RS23055 (BAT64_23045) | 19540..21693 | + | 2154 | WP_001235737 | type IA DNA topoisomerase | - |
| BAT64_RS23060 (BAT64_23050) | 21690..21896 | + | 207 | WP_000850859 | hemolysin expression modulator Hha | - |
Host bacterium
| ID | 3171 | GenBank | NZ_CP016575 |
| Plasmid name | pAMR588-04-00435_37 | Incompatibility group | IncX1 |
| Plasmid size | 37697 bp | Coordinate of oriT [Strand] | 3808..4414 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Heidelberg strain AMR588-04-00435 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |