Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102725 |
| Name | oriT_pSLy1  |
| Organism | Escherichia coli strain 210205630 isolate 210205630 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP015913 (21164..21216 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pSLy1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1652 | GenBank | WP_065304450 |
| Name | t4cp2_A9C00_RS25140_pSLy1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73526.12 Da Isoelectric Point: 9.2491
>WP_065304450.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNPVEIPLLFKDKTELMNKIARDAEIYLSDQKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43850..61187
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A9C00_RS31515 | 38923..39054 | + | 132 | WP_255639686 | hypothetical protein | - |
| A9C00_RS25080 (A9C00_25080) | 40004..40408 | - | 405 | WP_001175008 | IS200/IS605 family transposase | - |
| A9C00_RS25085 (A9C00_25085) | 40467..41693 | + | 1227 | WP_001672025 | RNA-guided endonuclease TnpB family protein | - |
| A9C00_RS31655 | 41741..41905 | - | 165 | Protein_50 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| A9C00_RS25090 (A9C00_25090) | 41924..43198 | - | 1275 | WP_235883108 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| A9C00_RS25095 (A9C00_25095) | 43211..43846 | - | 636 | WP_000934979 | A24 family peptidase | - |
| A9C00_RS25100 (A9C00_25100) | 43850..44332 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| A9C00_RS25105 (A9C00_25105) | 44398..44955 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| A9C00_RS25110 (A9C00_25110) | 45000..46109 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| A9C00_RS25115 (A9C00_25115) | 46100..47638 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| A9C00_RS25120 (A9C00_25120) | 47663..48157 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| A9C00_RS25125 (A9C00_25125) | 48141..49451 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| A9C00_RS25130 (A9C00_25130) | 49502..51145 | - | 1644 | WP_001035587 | PilN family type IVB pilus formation outer membrane protein | - |
| A9C00_RS25135 (A9C00_25135) | 51138..51668 | - | 531 | WP_001220542 | sigma 54-interacting transcriptional regulator | virb4 |
| A9C00_RS25140 (A9C00_25140) | 51715..53673 | - | 1959 | WP_065304450 | type IV secretory system conjugative DNA transfer family protein | - |
| A9C00_RS25145 (A9C00_25145) | 53689..54744 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| A9C00_RS25150 (A9C00_25150) | 54763..55902 | - | 1140 | WP_054173905 | TrbI/VirB10 family protein | virB10 |
| A9C00_RS25155 (A9C00_25155) | 55892..56593 | - | 702 | WP_049824867 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| A9C00_RS25160 (A9C00_25160) | 56659..57393 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| A9C00_RS25170 (A9C00_25170) | 57559..59916 | - | 2358 | WP_065304451 | VirB4 family type IV secretion system protein | virb4 |
| A9C00_RS25175 (A9C00_25175) | 59922..60242 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| A9C00_RS31410 (A9C00_25180) | 60313..60603 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| A9C00_RS25185 (A9C00_25185) | 60603..61187 | - | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| A9C00_RS25190 (A9C00_25190) | 61208..61606 | - | 399 | WP_001153665 | hypothetical protein | - |
| A9C00_RS25195 (A9C00_25195) | 61725..62162 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| A9C00_RS25200 (A9C00_25200) | 62168..63403 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| A9C00_RS25205 (A9C00_25205) | 63406..63705 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| A9C00_RS25210 (A9C00_25210) | 63773..64054 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| A9C00_RS25215 (A9C00_25215) | 64044..64295 | - | 252 | WP_000121741 | hypothetical protein | - |
| A9C00_RS25220 (A9C00_25220) | 64395..65030 | - | 636 | WP_015059536 | hypothetical protein | - |
| A9C00_RS25225 (A9C00_25225) | 65174..65887 | + | 714 | WP_065304452 | DUF5710 domain-containing protein | - |
Host bacterium
| ID | 3168 | GenBank | NZ_CP015913 |
| Plasmid name | pSLy1 | Incompatibility group | IncI2 |
| Plasmid size | 65888 bp | Coordinate of oriT [Strand] | 21164..21216 [-] |
| Host baterium | Escherichia coli strain 210205630 isolate 210205630 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |