Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102720
Name   oriT_TH1|unnamed1 in_silico
Organism   Klebsiella pneumoniae strain TH1 isolate TH1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP016160 (158065..158113 [+], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_TH1|unnamed1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2001 GenBank   WP_064081493
Name   traI_BAU11_RS26240_TH1|unnamed1 insolico UniProt ID   A0A2L1KT13
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190736.84 Da        Isoelectric Point: 6.4606

>WP_064081493.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTNGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILEPDNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRTGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMNAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIQ
KAVSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEKISVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGGAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGREGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLVGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(971-1157)

DNA helicase TraI (TraI_C)

(1433-1555)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

TrwC relaxase (TrwC)

(10-284)


  Protein structure



No available structure.




T4CP

ID   1643 GenBank   WP_064081494
Name   traD_BAU11_RS26245_TH1|unnamed1 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85901.94 Da        Isoelectric Point: 5.0596

>WP_064081494.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPESVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRVTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 128899..158672

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAU11_RS26245 (BAU11_26245) 128899..131211 - 2313 WP_064081494 type IV conjugative transfer system coupling protein TraD virb4
BAU11_RS30645 131276..131380 - 105 Protein_113 hypothetical protein -
BAU11_RS26250 (BAU11_26250) 131441..132649 - 1209 WP_071925340 RNA-guided endonuclease TnpB family protein -
BAU11_RS26255 (BAU11_26255) 132852..133583 - 732 WP_064081496 conjugal transfer complement resistance protein TraT -
BAU11_RS28290 133834..134436 - 603 WP_044264995 hypothetical protein -
BAU11_RS26260 (BAU11_26260) 134459..137281 - 2823 WP_064081497 conjugal transfer mating-pair stabilization protein TraG traG
BAU11_RS26265 (BAU11_26265) 137281..138660 - 1380 WP_074193588 conjugal transfer pilus assembly protein TraH traH
BAU11_RS26270 (BAU11_26270) 138638..139081 - 444 WP_014343488 F-type conjugal transfer protein TrbF -
BAU11_RS26275 (BAU11_26275) 139127..139684 - 558 WP_032437911 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
BAU11_RS26280 (BAU11_26280) 139656..139895 - 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
BAU11_RS26285 (BAU11_26285) 139906..140658 - 753 WP_064081499 type-F conjugative transfer system pilin assembly protein TraF traF
BAU11_RS26290 (BAU11_26290) 140679..141005 - 327 WP_064081500 hypothetical protein -
BAU11_RS30780 (BAU11_26295) 141051..141236 - 186 WP_064081501 hypothetical protein -
BAU11_RS26300 (BAU11_26300) 141233..141469 - 237 WP_042922352 conjugal transfer protein TrbE -
BAU11_RS26305 (BAU11_26305) 141501..143456 - 1956 WP_064081502 type-F conjugative transfer system mating-pair stabilization protein TraN traN
BAU11_RS26310 (BAU11_26310) 143515..144153 - 639 WP_046664202 type-F conjugative transfer system pilin assembly protein TrbC trbC
BAU11_RS26315 (BAU11_26315) 144166..145155 - 990 WP_064081503 conjugal transfer pilus assembly protein TraU traU
BAU11_RS26320 (BAU11_26320) 145152..145553 - 402 WP_004194979 hypothetical protein -
BAU11_RS26325 (BAU11_26325) 145588..146223 - 636 WP_032441885 type-F conjugative transfer system protein TraW traW
BAU11_RS26330 (BAU11_26330) 146223..146612 - 390 WP_040107933 type-F conjugative transfer system protein TrbI -
BAU11_RS26335 (BAU11_26335) 146612..149251 - 2640 WP_040107932 type IV secretion system protein TraC virb4
BAU11_RS26340 (BAU11_26340) 149323..149721 - 399 WP_074188112 hypothetical protein -
BAU11_RS28295 149729..150043 - 315 WP_225322590 hypothetical protein -
BAU11_RS26350 (BAU11_26350) 150085..150489 - 405 WP_032104551 hypothetical protein -
BAU11_RS26355 (BAU11_26355) 150556..150866 - 311 Protein_136 hypothetical protein -
BAU11_RS30275 (BAU11_26360) 150867..151085 - 219 WP_004195468 hypothetical protein -
BAU11_RS30455 (BAU11_26365) 151109..151399 - 291 WP_064081505 hypothetical protein -
BAU11_RS30650 151427..151825 - 399 WP_071925341 hypothetical protein -
BAU11_RS26370 (BAU11_26370) 151979..152563 - 585 WP_064081506 type IV conjugative transfer system lipoprotein TraV traV
BAU11_RS26375 (BAU11_26375) 152636..154060 - 1425 WP_064081507 F-type conjugal transfer pilus assembly protein TraB traB
BAU11_RS26380 (BAU11_26380) 154060..154800 - 741 WP_064081508 type-F conjugative transfer system secretin TraK traK
BAU11_RS26385 (BAU11_26385) 154787..155353 - 567 WP_004144423 type IV conjugative transfer system protein TraE traE
BAU11_RS26390 (BAU11_26390) 155373..155678 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
BAU11_RS26395 (BAU11_26395) 155692..156060 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
BAU11_RS26400 (BAU11_26400) 156122..156328 - 207 WP_171773970 TraY domain-containing protein -
BAU11_RS26405 (BAU11_26405) 156461..157180 - 720 WP_074193589 conjugal transfer protein TrbJ -
BAU11_RS26410 (BAU11_26410) 157380..157796 - 417 WP_074193590 conjugal transfer relaxosome DNA-binding protein TraM -
BAU11_RS26415 (BAU11_26415) 158187..158672 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
BAU11_RS26420 (BAU11_26420) 158705..159034 - 330 WP_074193592 DUF5983 family protein -
BAU11_RS26425 (BAU11_26425) 159067..159888 - 822 WP_064081511 DUF932 domain-containing protein -
BAU11_RS26435 (BAU11_26435) 160516..160866 - 351 WP_064081512 hypothetical protein -
BAU11_RS30295 (BAU11_26440) 160863..161135 - 273 WP_032445785 hypothetical protein -
BAU11_RS26445 (BAU11_26445) 161831..161953 - 123 WP_085842394 Hok/Gef family protein -
BAU11_RS30655 161898..162050 - 153 Protein_155 DUF5431 family protein -
BAU11_RS26450 (BAU11_26450) 162335..162820 - 486 WP_021312536 GNAT family N-acetyltransferase -
BAU11_RS26455 (BAU11_26455) 162808..163074 - 267 WP_003026799 DUF1778 domain-containing protein -
BAU11_RS28310 163207..163635 - 429 WP_004901287 GFA family protein -

Host bacterium

ID   3163 GenBank   NZ_CP016160
Plasmid name   TH1|unnamed1 Incompatibility group   IncFIA
Plasmid size   165276 bp Coordinate of oriT [Strand]   158065..158113 [+]
Host baterium   Klebsiella pneumoniae strain TH1 isolate TH1

Cargo genes

Drug resistance gene   -
Virulence gene   ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9