Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102719
Name   oriT_pL11989-5 in_silico
Organism   Loigolactobacillus backii strain TMW 1.1989
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP014878 (13385..13405 [-], 21 nt)
oriT length   21 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 21 nt

>oriT_pL11989-5
AGTGGGGTGTAAGTGCGCATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2000 GenBank   WP_068281344
Name   MobA_MobL_AYR53_RS13085_pL11989-5 insolico UniProt ID   A0A192H4K0
Length   687 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 687 a.a.        Molecular weight: 80529.95 Da        Isoelectric Point: 9.1467

>WP_068281344.1 MULTISPECIES: MobQ family relaxase [Loigolactobacillus]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDQEGRHYFYARSVIPESFILTPKNAPAWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDQGMVADVAIHRDHQDNPHAHVMLTNR
PFNPDGTWGIKSKKQYILDENGNKMYTGTSKYPKSRKILMVDWDKKEKIIEWRHNWAVSVNQVLEQKNIP
DRISEKSFTEQGIDDTPTQHEGINSKRYERKEFNQQVKNYRKAKASYKNNQEKAINRGHLDSLSEHFSFN
EKRVVNELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELNFEQTTQAERRELIKETESEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGFKLL
MQQEKEAKIDLKYTLMIHSDSLESLEHVDKGLLEKYSPAEQQTITRAVKDLRTIIAVKQVIQTQYHEVLK
RAFPNGDLDDLSLVRQEQAYTAVMYYDPTLKPLKVETMAQWQENPPRVFSTQEHQLGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFLGECKVDPTIKNSQIEKIQKQLKEQQAKDDQYRKSQLAHYQPLNYKPVSPNY
YLKTAFSDAIMTVLYARDEDYQRQRQAQGLKETEWEMAKKQRQHQTRNRHEDGGMHL

  Protein domains


Predicted by InterproScan.

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(276-384)

MobA/MobL family (MobA_MobL)

(17-241)


  Protein structure


Source ID Structure
AlphaFold DB A0A192H4K0


Host bacterium

ID   3162 GenBank   NZ_CP014878
Plasmid name   pL11989-5 Incompatibility group   -
Plasmid size   31925 bp Coordinate of oriT [Strand]   13385..13405 [-]
Host baterium   Loigolactobacillus backii strain TMW 1.1989

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -