Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102707 |
| Name | oriT_W25K|unnamed4  |
| Organism | Escherichia coli strain W25K |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP091039 (31838..32189 [+], 352 nt) |
| oriT length | 352 nt |
| IRs (inverted repeats) | 192..198, 206..212 (TATAAAA..TTTTATA) 197..202, 204..209 (AAAACA..TGTTTT) 104..110, 118..124 (TTTAAAT..ATTTAAA) 106..111, 113..118 (TAAATC..GATTTA) 40..47, 50..57 (GCAAAAAC..GTTTTTGC) 4..11, 16..23 (TTGGTGGT..ACCACCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 352 nt
>oriT_W25K|unnamed4
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGATTTTAATATCATATGCTTATATTCATGAATTTATATTGTTTAAATCAGATTTATTTAAAACAGCGGTGTAGGCGCGGCTATGGCACCGTGTCTGCACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTGTTTTATATTAGGGGTGCTGCTAGCTGCGCGGTGTGTTTTTTTATAGGATACTGCTAGGGGCGCTGCTAGCGGCGCGTTCCTGTTTTCATTGTGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTTAAAAAAGGTAATCTCTA
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGATTTTAATATCATATGCTTATATTCATGAATTTATATTGTTTAAATCAGATTTATTTAAAACAGCGGTGTAGGCGCGGCTATGGCACCGTGTCTGCACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTGTTTTATATTAGGGGTGCTGCTAGCTGCGCGGTGTGTTTTTTTATAGGATACTGCTAGGGGCGCTGCTAGCGGCGCGTTCCTGTTTTCATTGTGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTTAAAAAAGGTAATCTCTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 31267..38610
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L1O49_RS24505 (L1O49_24510) | 26406..26807 | + | 402 | Protein_33 | DUF1281 domain-containing protein | - |
| L1O49_RS24510 (L1O49_24515) | 26809..27174 | + | 366 | Protein_34 | hypothetical protein | - |
| L1O49_RS24515 (L1O49_24520) | 27229..27663 | + | 435 | WP_000845928 | conjugation system SOS inhibitor PsiB | - |
| L1O49_RS24520 (L1O49_24525) | 27660..28446 | + | 787 | Protein_36 | plasmid SOS inhibition protein A | - |
| L1O49_RS24525 (L1O49_24530) | 28596..28748 | + | 153 | Protein_37 | FlmC family protein | - |
| L1O49_RS24530 (L1O49_24535) | 28696..28815 | + | 120 | WP_228769536 | Hok/Gef family protein | - |
| L1O49_RS24535 (L1O49_24540) | 29281..29442 | - | 162 | Protein_39 | hypothetical protein | - |
| L1O49_RS24540 (L1O49_24545) | 29512..29718 | + | 207 | WP_000547959 | hypothetical protein | - |
| L1O49_RS24545 (L1O49_24550) | 29743..30030 | + | 288 | WP_000107534 | hypothetical protein | - |
| L1O49_RS24550 (L1O49_24555) | 30150..30971 | + | 822 | WP_001234475 | DUF932 domain-containing protein | - |
| L1O49_RS24555 (L1O49_24560) | 31267..31914 | - | 648 | WP_000601498 | transglycosylase SLT domain-containing protein | virB1 |
| L1O49_RS24560 (L1O49_24565) | 32190..32573 | + | 384 | WP_001151533 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| L1O49_RS24565 (L1O49_24570) | 32765..33412 | + | 648 | WP_000332517 | conjugal transfer transcriptional regulator TraJ | - |
| L1O49_RS24570 (L1O49_24575) | 33548..33763 | + | 216 | WP_000086380 | conjugal transfer relaxosome protein TraY | - |
| L1O49_RS24575 (L1O49_24580) | 33806..34168 | + | 363 | WP_000340279 | type IV conjugative transfer system pilin TraA | - |
| L1O49_RS24580 (L1O49_24585) | 34173..34484 | + | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
| L1O49_RS24585 (L1O49_24590) | 34506..35072 | + | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
| L1O49_RS24590 (L1O49_24595) | 35059..35787 | + | 729 | WP_001230788 | type-F conjugative transfer system secretin TraK | traK |
| L1O49_RS24595 (L1O49_24600) | 35787..37214 | + | 1428 | WP_000146688 | F-type conjugal transfer pilus assembly protein TraB | traB |
| L1O49_RS24600 (L1O49_24605) | 37204..37791 | + | 588 | WP_000002772 | conjugal transfer pilus-stabilizing protein TraP | - |
| L1O49_RS24605 (L1O49_24610) | 37778..38098 | + | 321 | WP_001057283 | conjugal transfer protein TrbD | - |
| L1O49_RS24610 (L1O49_24615) | 38095..38610 | + | 516 | WP_000724018 | type IV conjugative transfer system lipoprotein TraV | traV |
| L1O49_RS24615 (L1O49_24620) | 38745..38966 | + | 222 | WP_001278689 | conjugal transfer protein TraR | - |
| L1O49_RS24620 (L1O49_24625) | 39126..40585 | + | 1460 | Protein_56 | TraC family protein | - |
| L1O49_RS24625 (L1O49_24630) | 40581..40745 | + | 165 | Protein_57 | conjugal transfer protein TraU | - |
| L1O49_RS24630 (L1O49_24635) | 40742..41698 | + | 957 | Protein_58 | conjugal transfer pilus assembly protein TraU | - |
| L1O49_RS24635 (L1O49_24640) | 41703..42179 | + | 477 | Protein_59 | conjugal transfer protein TraD | - |
| L1O49_RS24640 (L1O49_24645) | 42178..42963 | + | 786 | Protein_60 | conjugal transfer protein TraI | - |
| L1O49_RS24645 (L1O49_24650) | 42962..43114 | - | 153 | Protein_61 | transposase | - |
Host bacterium
| ID | 3150 | GenBank | NZ_CP091039 |
| Plasmid name | W25K|unnamed4 | Incompatibility group | IncFII |
| Plasmid size | 80970 bp | Coordinate of oriT [Strand] | 31838..32189 [+] |
| Host baterium | Escherichia coli strain W25K |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | faeC, faeD, faeE, faeF, faeG, faeH, faeI, faeJ |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |