Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102706
• Name: oriT_INF298|6
• Organism: Klebsiella pneumoniae strain INF298 isolate INF298
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LR890431 (42048..42153 [+], 106 nt)
• oriT length: 106 nt
• IRs (inverted repeats): 30..35, 41..46 (CCGCCG..CGGCGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 106 nt

>oriT_INF298|6
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_JMX68_RS29975_INF298|6

• ID: 1992
• GenBank: WP_004187323
• Name: Relaxase_JMX68_RS29975_INF298|6
• UniProt ID: A0A3U8KUL3
• Length: 659 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 659 a.a.
• Molecular weight: 75179.89 Da
• Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (86-334)

Protein structure

• AlphaFold DB: A0A3U8KUL3

T4CP

t4cp2_JMX68_RS29675_INF298|6

• ID: 1635
• GenBank: WP_004206885
• Name: t4cp2_JMX68_RS29675_INF298|6
• UniProt ID: _
• Length: 695 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 695 a.a.
• Molecular weight: 79538.91 Da
• Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 44949..64238

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMX68_RS29955	40033..40344	+	312	WP_004187333	hypothetical protein	-
JMX68_RS29960	40477..41013	+	537	WP_004187332	hypothetical protein	-
JMX68_RS29965	41515..41910	-	396	WP_019725163	hypothetical protein	-
JMX68_RS30735	41945..42472	+	528	WP_172740549	plasmid mobilization protein MobA	-
JMX68_RS29975	42459..44438	+	1980	WP_004187323	TraI/MobA(P) family conjugative relaxase	-
JMX68_RS29980	44452..44952	+	501	WP_004187320	DotD/TraH family lipoprotein	-
JMX68_RS29985	44949..45728	+	780	WP_004187315	type IV secretory system conjugative DNA transfer family protein	traI
JMX68_RS29990	45739..46902	+	1164	WP_004187313	plasmid transfer ATPase TraJ	virB11
JMX68_RS29995	46892..47152	+	261	WP_004187310	IcmT/TraK family protein	traK
JMX68_RS30740	47177..50614	+	3438	WP_015586048	LPD7 domain-containing protein	-
JMX68_RS30005	50580..51092	+	513	WP_011091071	hypothetical protein	traL
JMX68_RS30010	51093..51248	-	156	WP_172693602	Hha/YmoA family nucleoid-associated regulatory protein	-
JMX68_RS30015	51277..51687	-	411	WP_004187465	H-NS family nucleoid-associated regulatory protein	-
JMX68_RS30020	51755..52468	+	714	WP_004187467	DotI/IcmL family type IV secretion protein	traM
JMX68_RS30025	52477..53628	+	1152	WP_004187471	DotH/IcmK family type IV secretion protein	traN
JMX68_RS30030	53640..54989	+	1350	WP_004187474	conjugal transfer protein TraO	traO
JMX68_RS30035	55001..55705	+	705	WP_015060002	conjugal transfer protein TraP	traP
JMX68_RS30040	55729..56259	+	531	WP_004187478	conjugal transfer protein TraQ	traQ
JMX68_RS30045	56276..56665	+	390	WP_004187479	DUF6750 family protein	traR
JMX68_RS30050	56711..57205	+	495	WP_004187480	hypothetical protein	-
JMX68_RS30055	57202..60252	+	3051	WP_004187482	hypothetical protein	traU
JMX68_RS30060	60249..61457	+	1209	WP_011091082	conjugal transfer protein TraW	traW
JMX68_RS30065	61454..62050	+	597	WP_015060003	hypothetical protein	-
JMX68_RS30070	62043..64238	+	2196	WP_015062834	DotA/TraY family protein	traY
JMX68_RS30075	64240..64893	+	654	WP_015060005	hypothetical protein	-
JMX68_RS30080	64974..65204	+	231	WP_011091085	IncL/M type plasmid replication protein RepC	-

Host bacterium

• ID: 3149
• GenBank: NZ_LR890431
• Plasmid name: INF298|6
• Incompatibility group: IncL/M
• Plasmid size: 65499 bp
• Coordinate of oriT [Strand]: 42048..42153 [+]
• Host baterium: Klebsiella pneumoniae strain INF298 isolate INF298

Cargo genes

• Drug resistance gene: blaOXA-48
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -