Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   102705
Name   oriT_INF298|5 in_silico
Organism   Klebsiella pneumoniae strain INF298 isolate INF298
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LR890430 (31496..31847 [+], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 104..109, 119..124  (TTTAAA..TTTAAA)
 93..98, 106..111  (GATTTA..TAAATC)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_INF298|5
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTTTTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1991 GenBank   WP_117389472
Name   traI_JMX68_RS29615_INF298|5 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191743.38 Da        Isoelectric Point: 5.7960

>WP_117389472.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTELAVREIAGQERDRSAISERETALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(1434-1557)

(633-712)

(10-283)

(575-626)

(968-1156)


  Protein structure



No available structure.




Auxiliary protein


ID   806 GenBank   WP_001151529
Name   WP_001151529_INF298|5 insolico UniProt ID   A0A7I0L277
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14497.49 Da        Isoelectric Point: 4.8662

>WP_001151529.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSAEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L277


T4CP


ID   1633 GenBank   WP_000069772
Name   traC_JMX68_RS29510_INF298|5 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99344.15 Da        Isoelectric Point: 6.4096

>WP_000069772.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(291-447)

(468-764)

(39-277)

  Protein structure



No available structure.



ID   1634 GenBank   WP_060578159
Name   traD_JMX68_RS29610_INF298|5 insolico UniProt ID   _
Length   735 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 735 a.a.        Molecular weight: 83556.20 Da        Isoelectric Point: 5.1652

>WP_060578159.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGHQGKQ
QSENEVTGGRQLTDNPKEVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
QENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(173-560)

(32-128)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 30925..59240

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMX68_RS29400 26915..27349 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
JMX68_RS29405 27346..28065 + 720 WP_001276217 plasmid SOS inhibition protein A -
JMX68_RS30725 28287..28436 + 150 Protein_36 plasmid maintenance protein Mok -
JMX68_RS29410 28378..28503 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
JMX68_RS29415 28804..29100 - 297 Protein_38 hypothetical protein -
JMX68_RS29420 29400..29696 + 297 WP_001272251 hypothetical protein -
JMX68_RS29425 29807..30628 + 822 WP_001234445 DUF932 domain-containing protein -
JMX68_RS29430 30925..31515 - 591 WP_000252683 transglycosylase SLT domain-containing protein virB1
JMX68_RS29435 31848..32231 + 384 WP_001151529 conjugal transfer relaxosome DNA-binding protein TraM -
JMX68_RS29440 32423..33070 + 648 WP_000332523 transcriptional regulator TraJ family protein -
JMX68_RS29445 33190..33417 + 228 WP_000589556 conjugal transfer relaxosome protein TraY -
JMX68_RS29450 33451..33813 + 363 WP_012881131 type IV conjugative transfer system pilin TraA -
JMX68_RS29455 33828..34139 + 312 WP_000012100 type IV conjugative transfer system protein TraL traL
JMX68_RS29460 34161..34727 + 567 WP_000399780 type IV conjugative transfer system protein TraE traE
JMX68_RS29465 34714..35442 + 729 WP_001230769 type-F conjugative transfer system secretin TraK traK
JMX68_RS29470 35442..36869 + 1428 WP_012881130 F-type conjugal transfer pilus assembly protein TraB traB
JMX68_RS29475 36859..37449 + 591 WP_012881129 conjugal transfer pilus-stabilizing protein TraP -
JMX68_RS29480 37436..37756 + 321 WP_012881128 conjugal transfer protein TrbD -
JMX68_RS29485 37753..38268 + 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
JMX68_RS29490 38403..38624 + 222 WP_001278683 conjugal transfer protein TraR -
JMX68_RS29495 38617..39093 + 477 WP_012881127 hypothetical protein -
JMX68_RS29500 39192..39410 + 219 WP_000556745 hypothetical protein -
JMX68_RS29505 39438..39785 + 348 WP_000836682 hypothetical protein -
JMX68_RS29510 39911..42541 + 2631 WP_000069772 type IV secretion system protein TraC virb4
JMX68_RS29515 42538..42924 + 387 WP_117389473 type-F conjugative transfer system protein TrbI -
JMX68_RS29520 42921..43553 + 633 WP_001203720 type-F conjugative transfer system protein TraW traW
JMX68_RS29525 43550..44542 + 993 WP_000830849 conjugal transfer pilus assembly protein TraU traU
JMX68_RS29530 44572..44877 + 306 WP_000224418 hypothetical protein -
JMX68_RS29535 44886..45524 + 639 WP_015058995 type-F conjugative transfer system pilin assembly protein TrbC trbC
JMX68_RS29540 45521..47371 + 1851 WP_117389471 type-F conjugative transfer system mating-pair stabilization protein TraN traN
JMX68_RS29545 47398..47655 + 258 WP_000864353 conjugal transfer protein TrbE -
JMX68_RS29550 47642..48391 + 750 WP_001336806 type-F conjugative transfer system pilin assembly protein TraF traF
JMX68_RS29555 48405..48746 + 342 WP_000556795 conjugal transfer protein TrbA -
JMX68_RS29560 48727..49062 - 336 WP_000736414 hypothetical protein -
JMX68_RS29565 49143..49427 + 285 WP_000624107 type-F conjugative transfer system pilin chaperone TraQ -
JMX68_RS29570 49414..49959 + 546 WP_000059830 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
JMX68_RS29575 49889..50236 + 348 WP_011666494 P-type conjugative transfer protein TrbJ -
JMX68_RS29580 50217..50609 + 393 WP_000659962 F-type conjugal transfer protein TrbF -
JMX68_RS29585 50596..51969 + 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
JMX68_RS29590 51966..54791 + 2826 WP_012881124 conjugal transfer mating-pair stabilization protein TraG traG
JMX68_RS29595 54788..55297 + 510 WP_000628111 conjugal transfer entry exclusion protein TraS -
JMX68_RS29600 55311..56042 + 732 WP_012881123 conjugal transfer complement resistance protein TraT -
JMX68_RS29605 56245..56982 + 738 WP_012881122 hypothetical protein -
JMX68_RS29610 57033..59240 + 2208 WP_060578159 type IV conjugative transfer system coupling protein TraD virb4


Host bacterium


ID   3148 GenBank   NZ_LR890430
Plasmid name   INF298|5 Incompatibility group   -
Plasmid size   70656 bp Coordinate of oriT [Strand]   31496..31847 [+]
Host baterium   Klebsiella pneumoniae strain INF298 isolate INF298

Cargo genes


Drug resistance gene   erm(B)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -